Transcript: Mouse NM_001085410.2

Mus musculus NAD kinase 2, mitochondrial (Nadk2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Nadk2 (68646)
Length:
3740
CDS:
243..1601

Additional Resources:

NCBI RefSeq record:
NM_001085410.2
NBCI Gene record:
Nadk2 (68646)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001085410.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360714 ACTACTTGAGCGACATCATAT pLKO_005 536 CDS 100% 13.200 18.480 N Nadk2 n/a
2 TRCN0000360715 TTCACCGTGAGACGGACTAAA pLKO_005 1771 3UTR 100% 13.200 18.480 N Nadk2 n/a
3 TRCN0000024980 CGACATCATATTCACACCAAA pLKO.1 546 CDS 100% 4.950 6.930 N Nadk2 n/a
4 TRCN0000360716 CTCTTTAGAGGCAGGTTAAAT pLKO_005 1853 3UTR 100% 15.000 10.500 N Nadk2 n/a
5 TRCN0000024983 GCTGTAGCAGTGGACAATTTA pLKO.1 1083 CDS 100% 15.000 10.500 N Nadk2 n/a
6 TRCN0000024981 CGCAGGCTGTTGAAGATGTTT pLKO.1 1255 CDS 100% 5.625 3.938 N Nadk2 n/a
7 TRCN0000024982 CGACAAGGAAATCTGACTCTT pLKO.1 1290 CDS 100% 4.950 3.465 N Nadk2 n/a
8 TRCN0000024979 GCCGAGCCTTTAACATCGAAA pLKO.1 952 CDS 100% 4.950 3.465 N Nadk2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001085410.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04884 pDONR223 100% 55.1% 58.8% None (many diffs) n/a
2 TRCN0000466703 ACAAATACCTATTAGCCAATGAGA pLX_317 50.6% 55.1% 58.8% V5 (many diffs) n/a
3 TRCN0000487878 CTCATTTACATACACTTAACTCTT pLX_317 35.4% 55.1% 58.8% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000489766 AGCCGATCCTTTATGCTTTCGTAC pLX_317 50.2% 55.1% 58.7% V5 (many diffs) n/a
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