Construct: ORF TRCN0000466717

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018959.3_s317c1
Derived from:
ccsbBroadEn_14933
DNA Barcode:
CATAATCGCACCGCATGCTGTAGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAGI1 (9223)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466717
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9223 MAGI1 membrane associated guanyla... NM_001033057.2 99.8% 15.3% (many diffs)
2 human 9223 MAGI1 membrane associated guanyla... NM_004742.3 81.7% 17.9% (many diffs)
3 human 9223 MAGI1 membrane associated guanyla... NM_001365905.1 81.6% 17.8% (many diffs)
4 human 9223 MAGI1 membrane associated guanyla... NM_015520.2 80.9% 17.4% (many diffs)
5 human 9223 MAGI1 membrane associated guanyla... NM_001365904.2 80.8% 17.4% (many diffs)
6 human 9223 MAGI1 membrane associated guanyla... NM_001365903.2 80.7% 17.4% (many diffs)
7 mouse 14924 Magi1 membrane associated guanyla... XM_006505600.1 89.2% 15.1% (many diffs)
8 mouse 14924 Magi1 membrane associated guanyla... XM_006505588.1 89.1% 15.1% (many diffs)
9 mouse 14924 Magi1 membrane associated guanyla... XM_006505587.1 89.1% 15.1% (many diffs)
10 mouse 14924 Magi1 membrane associated guanyla... XM_006505581.1 87.4% 14.8% (many diffs)
11 mouse 14924 Magi1 membrane associated guanyla... XM_006505582.1 87.4% 14.8% (many diffs)
12 mouse 14924 Magi1 membrane associated guanyla... XM_006505584.1 87.4% 14.8% (many diffs)
13 mouse 14924 Magi1 membrane associated guanyla... XM_006505580.1 87.4% 14.8% (many diffs)
14 mouse 14924 Magi1 membrane associated guanyla... XM_006505583.1 87.3% 14.8% (many diffs)
15 mouse 14924 Magi1 membrane associated guanyla... NM_001029850.4 86.8% 14.9% (many diffs)
16 mouse 14924 Magi1 membrane associated guanyla... NM_001286784.1 86.7% 14.9% (many diffs)
17 mouse 14924 Magi1 membrane associated guanyla... XM_006505585.1 86.7% 14.9% (many diffs)
18 mouse 14924 Magi1 membrane associated guanyla... XM_006505586.1 86.7% 14.9% (many diffs)
19 mouse 14924 Magi1 membrane associated guanyla... XM_006505590.1 84.7% 15.8% (many diffs)
20 mouse 14924 Magi1 membrane associated guanyla... XM_006505589.1 83.1% 15.5% (many diffs)
21 mouse 14924 Magi1 membrane associated guanyla... XM_011241222.2 74.9% 18.1% (many diffs)
22 mouse 14924 Magi1 membrane associated guanyla... XM_006505591.1 74.5% 16.6% (many diffs)
23 mouse 14924 Magi1 membrane associated guanyla... NM_001286786.1 73.5% 17.8% (many diffs)
24 mouse 14924 Magi1 membrane associated guanyla... XM_006505601.2 73.4% 17.2% (many diffs)
25 mouse 14924 Magi1 membrane associated guanyla... NM_001286785.1 73.4% 17.1% (many diffs)
26 mouse 14924 Magi1 membrane associated guanyla... XM_006505593.2 73.4% 17.1% (many diffs)
27 mouse 14924 Magi1 membrane associated guanyla... XM_006505592.1 73.3% 17.1% (many diffs)
28 mouse 14924 Magi1 membrane associated guanyla... XM_006505598.3 73.2% 3.9% (many diffs)
29 mouse 14924 Magi1 membrane associated guanyla... XM_006505599.3 73.2% 3.9% (many diffs)
30 mouse 14924 Magi1 membrane associated guanyla... NM_001083320.2 73.2% 4.4% (many diffs)
31 mouse 14924 Magi1 membrane associated guanyla... XM_006505594.3 73.2% 3.9% (many diffs)
32 mouse 14924 Magi1 membrane associated guanyla... XM_006505595.3 73.2% 3.9% (many diffs)
33 mouse 14924 Magi1 membrane associated guanyla... XM_006505596.2 73.2% 3.9% (many diffs)
34 mouse 14924 Magi1 membrane associated guanyla... XM_006505597.3 73.2% 3.9% (many diffs)
35 mouse 14924 Magi1 membrane associated guanyla... XM_011241223.2 73.2% 3.9% (many diffs)
36 mouse 14924 Magi1 membrane associated guanyla... XM_017321399.1 72.6% 17.3% (many diffs)
37 mouse 14924 Magi1 membrane associated guanyla... NM_010367.3 69.7% 18.7% (many diffs)
38 mouse 14924 Magi1 membrane associated guanyla... XM_017321400.1 69.1% 18.1% (many diffs)
39 mouse 14924 Magi1 membrane associated guanyla... NR_104594.1 63.7% (many diffs)
40 mouse 14924 Magi1 membrane associated guanyla... NM_001286788.1 60.1% 5.5% (many diffs)
41 mouse 14924 Magi1 membrane associated guanyla... NM_001083321.2 60% 4.8% (many diffs)
42 mouse 14924 Magi1 membrane associated guanyla... XR_377424.2 31% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
765
ORF length:
696
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtccaaagtg atccagaaga agaaccactg gactagcagg gttcacgaat
121gcaccgtgaa gcggggaccc cagggcgagc tgggggtgac ggtgctggga ggcgcggagc
181acggggagtt tccgtacgtc ggagcggtgg cggcggtcga ggcagcgggg cttcccggcg
241gcggcgaggg cccgaggctg ggcgaagggg agctgcttct ggaggtgcag ggggtccggg
301tgtccggctt gccccgctat gacgtgctgg gggtcatcga cagctgcaag gaggccgtca
361ccttcaaggc cgtcagacaa ggaggaaggc tgaacaagga cctgcgacat tttctcaatc
421agcgattcca gaaggggtct cctgatcatg agctccagca gaccataagg gataaccttt
481accgccatgc tgtgccttgc acaacccgat ctcccagaga aggagaagtg cctggcgtgg
541actataactt tctgactgtg aaggagttct tggacctcga gcagagtggg actcttctgg
601aagtcggcac ctatgaagga aactattatg ggacacccaa gcctcctagc cagccagtca
661gtgggaaagt gatcacgacg gatgccttgc acagccttca gtctggctct aagcagtcga
721ccccgaagcg aaccaagtcc tacatgatat gcaaaatgct ggcatagtcc acgcggagaa
781tgaggaggag gatgacgttc ctgaaatgaa cagcagcttt acagccgatt ctggtgaaca
841agaggagcac actctccaag aaacagcatt accacctgtg aatagtagca tcatcgctgc
901tcccatcacg gacccttctc agaagttccc tcaataccta cctctttctg cagaggataa
961tttaggtcct ctacctgaaa actgggagat ggcctatact gaaaatggag aagtctattt
1021tatagaccat aacacgaaaa caacatcttg gttagaccct cggtgcctaa acaagcagca
1081gaagccactg gaagagtgtg aagatgatga aggggtacac accgaggagc tggacagtga
1141actagaactg cctgctggtt gggaaaagat tgaagaccct gtctatggta tctactatgt
1201agaccacatc aacaggaaga cacaatatga gaacccggtt ctagaagcca aacggaagaa
1261gcagcttgag cagcagcagc agcagcaaca gcagcagcaa cagcagcagc agcagcagca
1321gcagcagcag acagaagaat ggacagaaga tcactcagcc cttgtgcctc ctgttattcc
1381aaaccaccct ccaagcaatc cagagccagc cagagaagtt ccacttcagg gcaaaccctt
1441ttttacacga aacccttctg agttgaaagg caagttcatt cacacaaagc tgcggaaaag
1501cagtcgtggc tttggcttca cggtggttgg aggggatgaa cctgatgagt ttctccagat
1561caagagcttg gtcctagatg gtcctgctgc attggatggc aagatggaaa caggggatgt
1621gattgtaagt gtgaatgaca cctgtgtttt gggacacaca catgctcaag ttgtgaagat
1681cttccagtcc atccccattg gtgccagcgt ggaccttgaa ctctgccgag gttatccatt
1741gccttttgat ccagatgacc ccaatacaag tttagtgacc tcggtagcca ttttggataa
1801agaaccaatt attgtgaatg ggcaagagac ctatgattca ccagctagcc acagtagtaa
1861aacaggcaaa gtcaatggca tgaaggatgc caggccaagc agcccagcgg acgtggcttc
1921aaatagttct catggttatc ctaatgacac tgtttctttg gcttcctcca tagccactca
1981gccagaactc ataactgttc atattgtcaa agggccaatg ggctttggtt ttactatcgc
2041agacagtcct ggtgggggtg gccaaagagt gaaacagatt gttgacagcc caaggtgccg
2101aggcctgaaa gaaggggatc tcatagtgga agttaataag aagaacgtgc aggccctaac
2161tcacaaccaa gtggtggata tgctggttga gtgtcctaag ggaagtgagg tcacattgtt
2221ggtgcaacga ggagggctgc cagttcccaa gaagagccca aagtcgcaac cactggaaag
2281gaaagacagc cagaatagtt ctcagcacag tgtttccagc caccgaagcc tgcacacagc
2341atccccaagc cacagcacac aggtgctccc cgagttccca cctgcagagg cccaagctcc
2401agatcaaact gacagctctg gccagaaaaa accagatcct tttaaaatct gggcccagtc
2461caggagcatg tatgaaaacc gacttccaga ttaccaggaa caggacatct tcctctggag
2521aaaagagact ggatttggat ttaggattct gggtggaaat gaaccagggg aacctattta
2581tattggtcac atcgtaccac tgggtgctgc tgatactgac ggccgcctga ggtctggaga
2641tgaattaatc tgtgtggatg ggacgccagt aattggaaaa tcacaccagc ttgtggtcca
2701gcttatgcaa caagctgcca agcaaggcca cgtcaatctc acggtgcggc gtaaagtggt
2761ttttgcggtg cccaaaaccg agaacgaggt gccctcgcca gcctcctctc atcacagtag
2821caaccagccg gcctcgctga cagaagagaa gcgcactccg cagggcagcc agaactcgct
2881gaacacggtg agctcgggca gcggcagcac cagcggcatc ggcagtggcg gcggcggggg
2941cagcggcgtg gtcagcaccg tggtgcagcc ctacgacgtg gagatccggc gcggggagaa
3001cgagggcttc ggcttcgtca tcgtgtcctc ggtgagcagg cccgaagcag gcacgacctt
3061tggcaatgca tgtgtggcta tgcctcacaa aataggtcgg attattgagg ggagccctgc
3121tgaccgctgt ggcaagctga aagtaggaga ccggatcttg gcagtaaatg gatgttccat
3181caccaacaaa tcccattcag acattgtgaa cctaatcaag gaagcgggaa acacagttac
3241cctccgcatc attcctgggg atgagtcctc gaatgccacc ttgctgacca atgcagagaa
3301gattgccacc atcaccacca cacacacccc ttctcagcaa gggacccagg agacaaggaa
3361taccaccaaa ccaaagcagg aatctcaatt tgagttcaaa gcaccccaag caacacagga
3421gcaagatttt tacactgtgg aactggaaag aggagccaag ggatttggct ttagccttcg
3481aggaggccga gagtataaca tggacctcta tgttctgcgc ttagcagagg acggtcctgc
3541ggagaggtgt ggaaagatga ggattggtga tgaaatttta gagatcaatg gtgagaccac
3601caaaaacatg aagcattctc gagctataga actgattaag aatggtggcc gcagagttcg
3661tctgtttctg aagcggggag acggctcagt accagattat gaccccagca gcgaccgcca
3721cggccccgcc accggtccac aaggtgttcc gaagtgaggc cgggccgacc gcggcagcat
3781cgtcattgga gtccagttac ccacccgatc ttcacaaatc atcaccacat ggggaaaagc
3841gggcacacgc aagggatccg aaaggcagca gagagtatag cagacaaccc aatgaacacc
3901acacctggaa tgggacttcg aggaaacccg acagcggggc atgccgaccc aaggaccggg
3961cgccggaggg acggagggac gcgcaggccg agcgcgcggc agccgccaac ggccccaaga
4021ggcggtcccc agagaagcgg agggagggca cccgcagcgc cgacaacact ttggagagga
4081gggagaagca cgagaagaga cgagacgtct ctccggagcg gaggcgagag cggtcaccca
4141cccgcaggag agacggctcc cccagccggc ggagacggtc tctggagaga ctcctggagc
4201agaggaggTC CCCCGAGCGC AGGAGAGGGG GCTCGCCCGA GCGCAGGGCC AAGTCCACCG
4261ACCGGAGGCG CGCACGCTCC CCCGAGCGCA GGAGAGAGCG GTCCCTGGAC AAAAGGAACA
4321GAGAGGACAG AGCCAGCCAC CGAGAAAGGG AAGAGGCGAA TCTGAAACAG GATGCCGGCA
4381GAAGTTCCAG ACATCCCCCG GAGCAGAGAA GGCGACCTTA CAAAGAATGT AGCACCGACC
4441TCAGTATCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
4501CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
4561TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACATAAT CGCACCGCAT GCTGTAGAAC
4621GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)