Construct: ORF TRCN0000466717
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018959.3_s317c1
- Derived from:
- ccsbBroadEn_14933
- DNA Barcode:
- CATAATCGCACCGCATGCTGTAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAGI1 (9223)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466717
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_001033057.2 | 99.8% | 15.3% | (many diffs) |
| 2 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_004742.3 | 81.7% | 17.9% | (many diffs) |
| 3 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_001365905.1 | 81.6% | 17.8% | (many diffs) |
| 4 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_015520.2 | 80.9% | 17.4% | (many diffs) |
| 5 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_001365904.2 | 80.8% | 17.4% | (many diffs) |
| 6 | human | 9223 | MAGI1 | membrane associated guanyla... | NM_001365903.2 | 80.7% | 17.4% | (many diffs) |
| 7 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505600.1 | 89.2% | 15.1% | (many diffs) |
| 8 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505588.1 | 89.1% | 15.1% | (many diffs) |
| 9 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505587.1 | 89.1% | 15.1% | (many diffs) |
| 10 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505581.1 | 87.4% | 14.8% | (many diffs) |
| 11 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505582.1 | 87.4% | 14.8% | (many diffs) |
| 12 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505584.1 | 87.4% | 14.8% | (many diffs) |
| 13 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505580.1 | 87.4% | 14.8% | (many diffs) |
| 14 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505583.1 | 87.3% | 14.8% | (many diffs) |
| 15 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001029850.4 | 86.8% | 14.9% | (many diffs) |
| 16 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001286784.1 | 86.7% | 14.9% | (many diffs) |
| 17 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505585.1 | 86.7% | 14.9% | (many diffs) |
| 18 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505586.1 | 86.7% | 14.9% | (many diffs) |
| 19 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505590.1 | 84.7% | 15.8% | (many diffs) |
| 20 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505589.1 | 83.1% | 15.5% | (many diffs) |
| 21 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_011241222.2 | 74.9% | 18.1% | (many diffs) |
| 22 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505591.1 | 74.5% | 16.6% | (many diffs) |
| 23 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001286786.1 | 73.5% | 17.8% | (many diffs) |
| 24 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505601.2 | 73.4% | 17.2% | (many diffs) |
| 25 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001286785.1 | 73.4% | 17.1% | (many diffs) |
| 26 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505593.2 | 73.4% | 17.1% | (many diffs) |
| 27 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505592.1 | 73.3% | 17.1% | (many diffs) |
| 28 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505598.3 | 73.2% | 3.9% | (many diffs) |
| 29 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505599.3 | 73.2% | 3.9% | (many diffs) |
| 30 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001083320.2 | 73.2% | 4.4% | (many diffs) |
| 31 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505594.3 | 73.2% | 3.9% | (many diffs) |
| 32 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505595.3 | 73.2% | 3.9% | (many diffs) |
| 33 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505596.2 | 73.2% | 3.9% | (many diffs) |
| 34 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_006505597.3 | 73.2% | 3.9% | (many diffs) |
| 35 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_011241223.2 | 73.2% | 3.9% | (many diffs) |
| 36 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_017321399.1 | 72.6% | 17.3% | (many diffs) |
| 37 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_010367.3 | 69.7% | 18.7% | (many diffs) |
| 38 | mouse | 14924 | Magi1 | membrane associated guanyla... | XM_017321400.1 | 69.1% | 18.1% | (many diffs) |
| 39 | mouse | 14924 | Magi1 | membrane associated guanyla... | NR_104594.1 | 63.7% | (many diffs) | |
| 40 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001286788.1 | 60.1% | 5.5% | (many diffs) |
| 41 | mouse | 14924 | Magi1 | membrane associated guanyla... | NM_001083321.2 | 60% | 4.8% | (many diffs) |
| 42 | mouse | 14924 | Magi1 | membrane associated guanyla... | XR_377424.2 | 31% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 765
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccaaagtg atccagaaga agaaccactg gactagcagg gttcacgaat 121 gcaccgtgaa gcggggaccc cagggcgagc tgggggtgac ggtgctggga ggcgcggagc 181 acggggagtt tccgtacgtc ggagcggtgg cggcggtcga ggcagcgggg cttcccggcg 241 gcggcgaggg cccgaggctg ggcgaagggg agctgcttct ggaggtgcag ggggtccggg 301 tgtccggctt gccccgctat gacgtgctgg gggtcatcga cagctgcaag gaggccgtca 361 ccttcaaggc cgtcagacaa ggaggaaggc tgaacaagga cctgcgacat tttctcaatc 421 agcgattcca gaaggggtct cctgatcatg agctccagca gaccataagg gataaccttt 481 accgccatgc tgtgccttgc acaacccgat ctcccagaga aggagaagtg cctggcgtgg 541 actataactt tctgactgtg aaggagttct tggacctcga gcagagtggg actcttctgg 601 aagtcggcac ctatgaagga aactattatg ggacacccaa gcctcctagc cagccagtca 661 gtgggaaagt gatcacgacg gatgccttgc acagccttca gtctggctct aagcagtcga 721 ccccgaagcg aaccaagtcc tacatgatat gcaaaatgct ggcatagtcc acgcggagaa 781 tgaggaggag gatgacgttc ctgaaatgaa cagcagcttt acagccgatt ctggtgaaca 841 agaggagcac actctccaag aaacagcatt accacctgtg aatagtagca tcatcgctgc 901 tcccatcacg gacccttctc agaagttccc tcaataccta cctctttctg cagaggataa 961 tttaggtcct ctacctgaaa actgggagat ggcctatact gaaaatggag aagtctattt 1021 tatagaccat aacacgaaaa caacatcttg gttagaccct cggtgcctaa acaagcagca 1081 gaagccactg gaagagtgtg aagatgatga aggggtacac accgaggagc tggacagtga 1141 actagaactg cctgctggtt gggaaaagat tgaagaccct gtctatggta tctactatgt 1201 agaccacatc aacaggaaga cacaatatga gaacccggtt ctagaagcca aacggaagaa 1261 gcagcttgag cagcagcagc agcagcaaca gcagcagcaa cagcagcagc agcagcagca 1321 gcagcagcag acagaagaat ggacagaaga tcactcagcc cttgtgcctc ctgttattcc 1381 aaaccaccct ccaagcaatc cagagccagc cagagaagtt ccacttcagg gcaaaccctt 1441 ttttacacga aacccttctg agttgaaagg caagttcatt cacacaaagc tgcggaaaag 1501 cagtcgtggc tttggcttca cggtggttgg aggggatgaa cctgatgagt ttctccagat 1561 caagagcttg gtcctagatg gtcctgctgc attggatggc aagatggaaa caggggatgt 1621 gattgtaagt gtgaatgaca cctgtgtttt gggacacaca catgctcaag ttgtgaagat 1681 cttccagtcc atccccattg gtgccagcgt ggaccttgaa ctctgccgag gttatccatt 1741 gccttttgat ccagatgacc ccaatacaag tttagtgacc tcggtagcca ttttggataa 1801 agaaccaatt attgtgaatg ggcaagagac ctatgattca ccagctagcc acagtagtaa 1861 aacaggcaaa gtcaatggca tgaaggatgc caggccaagc agcccagcgg acgtggcttc 1921 aaatagttct catggttatc ctaatgacac tgtttctttg gcttcctcca tagccactca 1981 gccagaactc ataactgttc atattgtcaa agggccaatg ggctttggtt ttactatcgc 2041 agacagtcct ggtgggggtg gccaaagagt gaaacagatt gttgacagcc caaggtgccg 2101 aggcctgaaa gaaggggatc tcatagtgga agttaataag aagaacgtgc aggccctaac 2161 tcacaaccaa gtggtggata tgctggttga gtgtcctaag ggaagtgagg tcacattgtt 2221 ggtgcaacga ggagggctgc cagttcccaa gaagagccca aagtcgcaac cactggaaag 2281 gaaagacagc cagaatagtt ctcagcacag tgtttccagc caccgaagcc tgcacacagc 2341 atccccaagc cacagcacac aggtgctccc cgagttccca cctgcagagg cccaagctcc 2401 agatcaaact gacagctctg gccagaaaaa accagatcct tttaaaatct gggcccagtc 2461 caggagcatg tatgaaaacc gacttccaga ttaccaggaa caggacatct tcctctggag 2521 aaaagagact ggatttggat ttaggattct gggtggaaat gaaccagggg aacctattta 2581 tattggtcac atcgtaccac tgggtgctgc tgatactgac ggccgcctga ggtctggaga 2641 tgaattaatc tgtgtggatg ggacgccagt aattggaaaa tcacaccagc ttgtggtcca 2701 gcttatgcaa caagctgcca agcaaggcca cgtcaatctc acggtgcggc gtaaagtggt 2761 ttttgcggtg cccaaaaccg agaacgaggt gccctcgcca gcctcctctc atcacagtag 2821 caaccagccg gcctcgctga cagaagagaa gcgcactccg cagggcagcc agaactcgct 2881 gaacacggtg agctcgggca gcggcagcac cagcggcatc ggcagtggcg gcggcggggg 2941 cagcggcgtg gtcagcaccg tggtgcagcc ctacgacgtg gagatccggc gcggggagaa 3001 cgagggcttc ggcttcgtca tcgtgtcctc ggtgagcagg cccgaagcag gcacgacctt 3061 tggcaatgca tgtgtggcta tgcctcacaa aataggtcgg attattgagg ggagccctgc 3121 tgaccgctgt ggcaagctga aagtaggaga ccggatcttg gcagtaaatg gatgttccat 3181 caccaacaaa tcccattcag acattgtgaa cctaatcaag gaagcgggaa acacagttac 3241 cctccgcatc attcctgggg atgagtcctc gaatgccacc ttgctgacca atgcagagaa 3301 gattgccacc atcaccacca cacacacccc ttctcagcaa gggacccagg agacaaggaa 3361 taccaccaaa ccaaagcagg aatctcaatt tgagttcaaa gcaccccaag caacacagga 3421 gcaagatttt tacactgtgg aactggaaag aggagccaag ggatttggct ttagccttcg 3481 aggaggccga gagtataaca tggacctcta tgttctgcgc ttagcagagg acggtcctgc 3541 ggagaggtgt ggaaagatga ggattggtga tgaaatttta gagatcaatg gtgagaccac 3601 caaaaacatg aagcattctc gagctataga actgattaag aatggtggcc gcagagttcg 3661 tctgtttctg aagcggggag acggctcagt accagattat gaccccagca gcgaccgcca 3721 cggccccgcc accggtccac aaggtgttcc gaagtgaggc cgggccgacc gcggcagcat 3781 cgtcattgga gtccagttac ccacccgatc ttcacaaatc atcaccacat ggggaaaagc 3841 gggcacacgc aagggatccg aaaggcagca gagagtatag cagacaaccc aatgaacacc 3901 acacctggaa tgggacttcg aggaaacccg acagcggggc atgccgaccc aaggaccggg 3961 cgccggaggg acggagggac gcgcaggccg agcgcgcggc agccgccaac ggccccaaga 4021 ggcggtcccc agagaagcgg agggagggca cccgcagcgc cgacaacact ttggagagga 4081 gggagaagca cgagaagaga cgagacgtct ctccggagcg gaggcgagag cggtcaccca 4141 cccgcaggag agacggctcc cccagccggc ggagacggtc tctggagaga ctcctggagc 4201 agaggaggTC CCCCGAGCGC AGGAGAGGGG GCTCGCCCGA GCGCAGGGCC AAGTCCACCG 4261 ACCGGAGGCG CGCACGCTCC CCCGAGCGCA GGAGAGAGCG GTCCCTGGAC AAAAGGAACA 4321 GAGAGGACAG AGCCAGCCAC CGAGAAAGGG AAGAGGCGAA TCTGAAACAG GATGCCGGCA 4381 GAAGTTCCAG ACATCCCCCG GAGCAGAGAA GGCGACCTTA CAAAGAATGT AGCACCGACC 4441 TCAGTATCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 4501 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 4561 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACATAAT CGCACCGCAT GCTGTAGAAC 4621 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt