Construct: ORF TRCN0000466717

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018959.3_s317c1
Derived from:
ccsbBroadEn_14933
DNA Barcode:
CATAATCGCACCGCATGCTGTAGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAGI1 (9223)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466717
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9223 MAGI1 membrane associated guanyla... NM_001033057.2 99.8% 15.3% (many diffs)
2 human 9223 MAGI1 membrane associated guanyla... NM_004742.3 81.7% 17.9% (many diffs)
3 human 9223 MAGI1 membrane associated guanyla... NM_001365905.1 81.6% 17.8% (many diffs)
4 human 9223 MAGI1 membrane associated guanyla... NM_015520.2 80.9% 17.4% (many diffs)
5 human 9223 MAGI1 membrane associated guanyla... NM_001365904.2 80.8% 17.4% (many diffs)
6 human 9223 MAGI1 membrane associated guanyla... NM_001365903.2 80.7% 17.4% (many diffs)
7 mouse 14924 Magi1 membrane associated guanyla... XM_006505600.1 89.2% 15.1% (many diffs)
8 mouse 14924 Magi1 membrane associated guanyla... XM_006505588.1 89.1% 15.1% (many diffs)
9 mouse 14924 Magi1 membrane associated guanyla... XM_006505587.1 89.1% 15.1% (many diffs)
10 mouse 14924 Magi1 membrane associated guanyla... XM_006505581.1 87.4% 14.8% (many diffs)
11 mouse 14924 Magi1 membrane associated guanyla... XM_006505582.1 87.4% 14.8% (many diffs)
12 mouse 14924 Magi1 membrane associated guanyla... XM_006505584.1 87.4% 14.8% (many diffs)
13 mouse 14924 Magi1 membrane associated guanyla... XM_006505580.1 87.4% 14.8% (many diffs)
14 mouse 14924 Magi1 membrane associated guanyla... XM_006505583.1 87.3% 14.8% (many diffs)
15 mouse 14924 Magi1 membrane associated guanyla... NM_001029850.4 86.8% 14.9% (many diffs)
16 mouse 14924 Magi1 membrane associated guanyla... NM_001286784.1 86.7% 14.9% (many diffs)
17 mouse 14924 Magi1 membrane associated guanyla... XM_006505585.1 86.7% 14.9% (many diffs)
18 mouse 14924 Magi1 membrane associated guanyla... XM_006505586.1 86.7% 14.9% (many diffs)
19 mouse 14924 Magi1 membrane associated guanyla... XM_006505590.1 84.7% 15.8% (many diffs)
20 mouse 14924 Magi1 membrane associated guanyla... XM_006505589.1 83.1% 15.5% (many diffs)
21 mouse 14924 Magi1 membrane associated guanyla... XM_011241222.2 74.9% 18.1% (many diffs)
22 mouse 14924 Magi1 membrane associated guanyla... XM_006505591.1 74.5% 16.6% (many diffs)
23 mouse 14924 Magi1 membrane associated guanyla... NM_001286786.1 73.5% 17.8% (many diffs)
24 mouse 14924 Magi1 membrane associated guanyla... XM_006505601.2 73.4% 17.2% (many diffs)
25 mouse 14924 Magi1 membrane associated guanyla... NM_001286785.1 73.4% 17.1% (many diffs)
26 mouse 14924 Magi1 membrane associated guanyla... XM_006505593.2 73.4% 17.1% (many diffs)
27 mouse 14924 Magi1 membrane associated guanyla... XM_006505592.1 73.3% 17.1% (many diffs)
28 mouse 14924 Magi1 membrane associated guanyla... XM_006505598.3 73.2% 3.9% (many diffs)
29 mouse 14924 Magi1 membrane associated guanyla... XM_006505599.3 73.2% 3.9% (many diffs)
30 mouse 14924 Magi1 membrane associated guanyla... NM_001083320.2 73.2% 4.4% (many diffs)
31 mouse 14924 Magi1 membrane associated guanyla... XM_006505594.3 73.2% 3.9% (many diffs)
32 mouse 14924 Magi1 membrane associated guanyla... XM_006505595.3 73.2% 3.9% (many diffs)
33 mouse 14924 Magi1 membrane associated guanyla... XM_006505596.2 73.2% 3.9% (many diffs)
34 mouse 14924 Magi1 membrane associated guanyla... XM_006505597.3 73.2% 3.9% (many diffs)
35 mouse 14924 Magi1 membrane associated guanyla... XM_011241223.2 73.2% 3.9% (many diffs)
36 mouse 14924 Magi1 membrane associated guanyla... XM_017321399.1 72.6% 17.3% (many diffs)
37 mouse 14924 Magi1 membrane associated guanyla... NM_010367.3 69.7% 18.7% (many diffs)
38 mouse 14924 Magi1 membrane associated guanyla... XM_017321400.1 69.1% 18.1% (many diffs)
39 mouse 14924 Magi1 membrane associated guanyla... NR_104594.1 63.7% (many diffs)
40 mouse 14924 Magi1 membrane associated guanyla... NM_001286788.1 60.1% 5.5% (many diffs)
41 mouse 14924 Magi1 membrane associated guanyla... NM_001083321.2 60% 4.8% (many diffs)
42 mouse 14924 Magi1 membrane associated guanyla... XR_377424.2 31% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
765
ORF length:
696
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtccaaagtg atccagaaga agaaccactg gactagcagg gttcacgaat
121gcaccgtgaa gcggggaccc cagggcgagc tgggggtgac ggtgctggga ggcgcggagc
181acggggagtt tccgtacgtc ggagcggtgg cggcggtcga ggcagcgggg cttcccggcg
241gcggcgaggg cccgaggctg ggcgaagggg agctgcttct ggaggtgcag ggggtccggg
301tgtccggctt gccccgctat gacgtgctgg gggtcatcga cagctgcaag gaggccgtca
361ccttcaaggc cgtcagacaa ggaggaaggc tgaacaagga cctgcgacat tttctcaatc
421agcgattcca gaaggggtct cctgatcatg agctccagca gaccataagg gataaccttt
481accgccatgc tgtgccttgc acaacccgat ctcccagaga aggagaagtg cctggcgtgg
541actataactt tctgactgtg aaggagttct tggacctcga gcagagtggg actcttctgg
601aagtcggcac ctatgaagga aactattatg ggacacccaa gcctcctagc cagccagtca
661gtgggaaagt gatcacgacg gatgccttgc acagccttca gtctggctct aagcagtcga
721ccccgaagcg aaccaagtcc tacatgatat gcaaaatgct ggcatagtcc acgcggagaa
781tgaggaggag gatgacgttc ctgaaatgaa cagcagcttt acagccgatt ctggtgaaca
841agaggagcac actctccaag aaacagcatt accacctgtg aatagtagca tcatcgctgc
901tcccatcacg gacccttctc agaagttccc tcaataccta cctctttctg cagaggataa
961tttaggtcct ctacctgaaa actgggagat ggcctatact gaaaatggag aagtctattt
1021tatagaccat aacacgaaaa caacatcttg gttagaccct cggtgcctaa acaagcagca
1081gaagccactg gaagagtgtg aagatgatga aggggtacac accgaggagc tggacagtga
1141actagaactg cctgctggtt gggaaaagat tgaagaccct gtctatggta tctactatgt
1201agaccacatc aacaggaaga cacaatatga gaacccggtt ctagaagcca aacggaagaa
1261gcagcttgag cagcagcagc agcagcaaca gcagcagcaa cagcagcagc agcagcagca
1321gcagcagcag acagaagaat ggacagaaga tcactcagcc cttgtgcctc ctgttattcc
1381aaaccaccct ccaagcaatc cagagccagc cagagaagtt ccacttcagg gcaaaccctt
1441ttttacacga aacccttctg agttgaaagg caagttcatt cacacaaagc tgcggaaaag
1501cagtcgtggc tttggcttca cggtggttgg aggggatgaa cctgatgagt ttctccagat
1561caagagcttg gtcctagatg gtcctgctgc attggatggc aagatggaaa caggggatgt
1621gattgtaagt gtgaatgaca cctgtgtttt gggacacaca catgctcaag ttgtgaagat
1681cttccagtcc atccccattg gtgccagcgt ggaccttgaa ctctgccgag gttatccatt
1741gccttttgat ccagatgacc ccaatacaag tttagtgacc tcggtagcca ttttggataa
1801agaaccaatt attgtgaatg ggcaagagac ctatgattca ccagctagcc acagtagtaa
1861aacaggcaaa gtcaatggca tgaaggatgc caggccaagc agcccagcgg acgtggcttc
1921aaatagttct catggttatc ctaatgacac tgtttctttg gcttcctcca tagccactca
1981gccagaactc ataactgttc atattgtcaa agggccaatg ggctttggtt ttactatcgc
2041agacagtcct ggtgggggtg gccaaagagt gaaacagatt gttgacagcc caaggtgccg
2101aggcctgaaa gaaggggatc tcatagtgga agttaataag aagaacgtgc aggccctaac
2161tcacaaccaa gtggtggata tgctggttga gtgtcctaag ggaagtgagg tcacattgtt
2221ggtgcaacga ggagggctgc cagttcccaa gaagagccca aagtcgcaac cactggaaag
2281gaaagacagc cagaatagtt ctcagcacag tgtttccagc caccgaagcc tgcacacagc
2341atccccaagc cacagcacac aggtgctccc cgagttccca cctgcagagg cccaagctcc
2401agatcaaact gacagctctg gccagaaaaa accagatcct tttaaaatct gggcccagtc
2461caggagcatg tatgaaaacc gacttccaga ttaccaggaa caggacatct tcctctggag
2521aaaagagact ggatttggat ttaggattct gggtggaaat gaaccagggg aacctattta
2581tattggtcac atcgtaccac tgggtgctgc tgatactgac ggccgcctga ggtctggaga
2641tgaattaatc tgtgtggatg ggacgccagt aattggaaaa tcacaccagc ttgtggtcca
2701gcttatgcaa caagctgcca agcaaggcca cgtcaatctc acggtgcggc gtaaagtggt
2761ttttgcggtg cccaaaaccg agaacgaggt gccctcgcca gcctcctctc atcacagtag
2821caaccagccg gcctcgctga cagaagagaa gcgcactccg cagggcagcc agaactcgct
2881gaacacggtg agctcgggca gcggcagcac cagcggcatc ggcagtggcg gcggcggggg
2941cagcggcgtg gtcagcaccg tggtgcagcc ctacgacgtg gagatccggc gcggggagaa
3001cgagggcttc ggcttcgtca tcgtgtcctc ggtgagcagg cccgaagcag gcacgacctt
3061tggcaatgca tgtgtggcta tgcctcacaa aataggtcgg attattgagg ggagccctgc
3121tgaccgctgt ggcaagctga aagtaggaga ccggatcttg gcagtaaatg gatgttccat
3181caccaacaaa tcccattcag acattgtgaa cctaatcaag gaagcgggaa acacagttac
3241cctccgcatc attcctgggg atgagtcctc gaatgccacc ttgctgacca atgcagagaa
3301gattgccacc atcaccacca cacacacccc ttctcagcaa gggacccagg agacaaggaa
3361taccaccaaa ccaaagcagg aatctcaatt tgagttcaaa gcaccccaag caacacagga
3421gcaagatttt tacactgtgg aactggaaag aggagccaag ggatttggct ttagccttcg
3481aggaggccga gagtataaca tggacctcta tgttctgcgc ttagcagagg acggtcctgc
3541ggagaggtgt ggaaagatga ggattggtga tgaaatttta gagatcaatg gtgagaccac
3601caaaaacatg aagcattctc gagctataga actgattaag aatggtggcc gcagagttcg
3661tctgtttctg aagcggggag acggctcagt accagattat gaccccagca gcgaccgcca
3721cggccccgcc accggtccac aaggtgttcc gaagtgaggc cgggccgacc gcggcagcat
3781cgtcattgga gtccagttac ccacccgatc ttcacaaatc atcaccacat ggggaaaagc
3841gggcacacgc aagggatccg aaaggcagca gagagtatag cagacaaccc aatgaacacc
3901acacctggaa tgggacttcg aggaaacccg acagcggggc atgccgaccc aaggaccggg
3961cgccggaggg acggagggac gcgcaggccg agcgcgcggc agccgccaac ggccccaaga
4021ggcggtcccc agagaagcgg agggagggca cccgcagcgc cgacaacact ttggagagga
4081gggagaagca cgagaagaga cgagacgtct ctccggagcg gaggcgagag cggtcaccca
4141cccgcaggag agacggctcc cccagccggc ggagacggtc tctggagaga ctcctggagc
4201agaggaggTC CCCCGAGCGC AGGAGAGGGG GCTCGCCCGA GCGCAGGGCC AAGTCCACCG
4261ACCGGAGGCG CGCACGCTCC CCCGAGCGCA GGAGAGAGCG GTCCCTGGAC AAAAGGAACA
4321GAGAGGACAG AGCCAGCCAC CGAGAAAGGG AAGAGGCGAA TCTGAAACAG GATGCCGGCA
4381GAAGTTCCAG ACATCCCCCG GAGCAGAGAA GGCGACCTTA CAAAGAATGT AGCACCGACC
4441TCAGTATCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
4501CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
4561TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACATAAT CGCACCGCAT GCTGTAGAAC
4621GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.