Construct: ORF TRCN0000466745
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006066.1_s317c1
- Derived from:
- ccsbBroadEn_08018
- DNA Barcode:
- GTAAGCTGTGGGATGTCCCGAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NARF (26502)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466745
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26502 | NARF | nuclear prelamin A recognit... | NM_001083608.2 | 99.9% | 99.7% | 10G>A |
| 2 | human | 26502 | NARF | nuclear prelamin A recognit... | NM_001038618.3 | 91.4% | 89.4% | (many diffs) |
| 3 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_005256340.4 | 91.4% | 89.4% | (many diffs) |
| 4 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_011523541.2 | 91.4% | 89.4% | (many diffs) |
| 5 | human | 26502 | NARF | nuclear prelamin A recognit... | NM_012336.4 | 89.4% | 89.2% | 10G>A;107_250del |
| 6 | human | 26502 | NARF | nuclear prelamin A recognit... | NM_031968.2 | 81.2% | 81% | 10G>A;107_250del;768_905del |
| 7 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_006722278.4 | 79.5% | 79.3% | 10G>A;107_250del;383_384ins135 |
| 8 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_017024449.2 | 63.8% | 61.9% | (many diffs) |
| 9 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_011523543.3 | 62.7% | 62.7% | 0_1ins456 |
| 10 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_017024450.2 | 62.7% | 62.7% | 0_1ins456 |
| 11 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_011523544.2 | 42.1% | 36.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1290
- ORF length:
- 1224
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtgtaagcac tgcacgcgca aggaatgtag taagaaaaca aaaactgatg 121 accaagagaa tgtgtcagcc gatgcaccga gtccagccca ggaaaatgga gagaaatgtg 181 atacctcaaa gcacaaagtg ctggtagtgt ctgtgtgtcc tcaatctttg ccttattttg 241 ctgctaaatt caacctcagt gtaactgatg catccagaag actctgtggt ttcctcaaaa 301 gtcttggggt gcactatgta tttgatacga cgatagctgc ggattttagt atcctggaga 361 gtcaaaaaga attcgtgcgt cgctatcgcc agcacagtga ggaggaacgc accctgccca 421 tgctgacctc tgcctgtcct ggctgggtcc gatacgccga gcgggtgctg ggtcgcccca 481 tcactgccca cctctgcacc gccaagtccc cccagcaggt catgggctct ttggtgaagg 541 attatttcgc cagacagcag aacctgtctc cagagaagat tttccacgtc attgtggccc 601 cttgttatga caagaagctg gaggctcttc aggaaagcct tccccctgct ttgcatggct 661 cccggggcgc tgactgcgtg ttaacatcag gtgaaattgc tcaaataatg gagcaaggtg 721 acctctcagt gagagatgct gccgtcgaca ctctgtttgg agacttgaag gaggacaaag 781 tgacgcgtca tgatggagcc agctcagacg ggcacctggc acacatcttc agacatgcgg 841 ccaaggagct gttcaacgag gatgtggagg aggtcactta ccgagccctg agaaacaaag 901 acttccaaga ggtcaccctt gagaagaacg gagaggtggt gttacgcttt gctgcagcct 961 atggctttcg aaacaTCCAG AACATGATCC TGAAGCTTAA GAAGGGCAAG TTCCCATTCC 1021 ACTTTGTGGA GGTCCTCGCC TGTGCTGGAG GATGCTTAAA TGGCAGAGGC CAAGCCCAGA 1081 CTCCAGACGG ACATGCGGAT AAGGCCCTGC TGCGGCAGAT GGAAGGCATT TACGCTGACA 1141 TCCCTGTGCG GCGTCCGGAG TCCAGTGCAC ACGTGCAGGA GCTGTACCAG GAGTGGCTGG 1201 AGGGGATCAA CTCCCCCAAG GCCCGAGAGG TGCTGCATAC CACGTACCAG AGCCAGGAGC 1261 GTGGCACACA CAGCCTGGAC ATCAAGTGGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1321 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1381 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGTAAG 1441 CTGTGGGATG TCCCGAATGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1501 tgaaagatt