Construct: ORF TRCN0000466754
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001958.1_s317c1
- Derived from:
- ccsbBroadEn_00374
- DNA Barcode:
- AAACTGCTGAAATAACCAGGGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSH2 (1443)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466754
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1443 | CSH2 | chorionic somatomammotropin... | NM_020991.3 | 100% | 100% | |
| 2 | human | 1442 | CSH1 | chorionic somatomammotropin... | NM_001317.6 | 99.2% | 99.5% | (many diffs) |
| 3 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022579.3 | 93.8% | 90.5% | (many diffs) |
| 4 | human | 2688 | GH1 | growth hormone 1 | NM_000515.5 | 92.4% | 85.2% | (many diffs) |
| 5 | human | 2689 | GH2 | growth hormone 2 | NM_002059.5 | 90.1% | 80.1% | (many diffs) |
| 6 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022581.3 | 86.6% | 83.4% | (many diffs) |
| 7 | human | 2688 | GH1 | growth hormone 1 | NM_022559.4 | 86.3% | 79.7% | (many diffs) |
| 8 | human | 2689 | GH2 | growth hormone 2 | NM_022556.4 | 84.3% | 75.1% | (many diffs) |
| 9 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321069.2 | 81.2% | 78.3% | (many diffs) |
| 10 | human | 2689 | GH2 | growth hormone 2 | NM_022558.4 | 79% | 47% | (many diffs) |
| 11 | human | 2688 | GH1 | growth hormone 1 | NM_022560.4 | 75.5% | 70.5% | (many diffs) |
| 12 | human | 1443 | CSH2 | chorionic somatomammotropin... | NM_022644.3 | 74.1% | 71.5% | (many diffs) |
| 13 | human | 1444 | CSHL1 | chorionic somatomammotropin... | XM_011524344.1 | 71.6% | 55.9% | (many diffs) |
| 14 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321067.2 | 71.2% | 67.2% | (many diffs) |
| 15 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321068.2 | 71.1% | 69.1% | (many diffs) |
| 16 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022580.3 | 61.1% | 58% | (many diffs) |
| 17 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001318.4 | 56.5% | 54.8% | (many diffs) |
| 18 | human | 1443 | CSH2 | chorionic somatomammotropin... | NM_022645.2 | 56.2% | 56.2% | 171_172ins285 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcaggctcc cggacgtccc tgctcctggc ttttgccctg ctctgcctgc 121 cctggcttca agaggctggt gccgtccaaa ccgttccgtt atccaggctt tttgaccacg 181 ctatgctcca agcccatcgc gcgcaccagc tggccattga cacctaccag gagtttgaag 241 aaacctatat cccaaaggac cagaagtatt cattcctgca tgactcccag acctccttct 301 gcttctcaga ctctatTCCG ACACCCTCCA ACATGGAGGA AACGCAACAG AAATCCAATC 361 TAGAGCTGCT CCGCATCTCC CTGCTGCTCA TCGAGTCGTG GCTGGAGCCC GTGCGGTTCC 421 TCAGGAGTAT GTTCGCCAAC AACCTGGTGT ATGACACCTC GGACAGCGAT GACTATCACC 481 TCCTAAAGGA CCTAGAGGAA GGCATCCAAA CGCTGATGGG GAGGCTGGAA GACGGCAGCC 541 GCCGGACTGG GCAGATCCTC AAGCAGACCT ACAGCAAGTT TGACACAAAC TCACACAACC 601 ATGACGCACT GCTCAAGAAC TACGGGCTGC TCTACTGCTT CAGGAAGGAC ATGGACAAGG 661 TCGAGACATT CCTGCGCATG GTGCAGTGCC GCTCTGTAGA GGGTAGCTGT GGCTTCTACC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GAAAACTGCT GAAATAACCA GGGAGGACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt