Construct: ORF TRCN0000466770
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015387.1_s317c1
- Derived from:
- ccsbBroadEn_10521
- DNA Barcode:
- CACCAGTATCGGGACTCGTTCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATAT1 (79969)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466770
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79969 | ATAT1 | alpha tubulin acetyltransfe... | NM_001318763.2 | 54.9% | 53.2% | (many diffs) |
| 2 | human | 79969 | ATAT1 | alpha tubulin acetyltransfe... | NM_024909.4 | 53.2% | 51.6% | (many diffs) |
| 3 | human | 79969 | ATAT1 | alpha tubulin acetyltransfe... | NM_001190724.3 | 49.6% | 47.5% | (many diffs) |
| 4 | human | 79969 | ATAT1 | alpha tubulin acetyltransfe... | NM_001318762.2 | 47.7% | 45.8% | (many diffs) |
| 5 | human | 79969 | ATAT1 | alpha tubulin acetyltransfe... | NM_001254952.3 | 46.3% | 44.4% | (many diffs) |
| 6 | human | 79969 | ATAT1 | alpha tubulin acetyltransfe... | NM_001031722.4 | 43.3% | 42% | (many diffs) |
| 7 | human | 79969 | ATAT1 | alpha tubulin acetyltransfe... | NR_033823.3 | 34.1% | 1_219del;337G>T;757_1571del | |
| 8 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | XM_017317676.1 | 54.3% | 55.8% | (many diffs) |
| 9 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | XM_006524991.3 | 51.3% | 48.4% | (many diffs) |
| 10 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | XR_385372.3 | 51.2% | (many diffs) | |
| 11 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | NM_001142745.1 | 47.3% | 48.6% | (many diffs) |
| 12 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | XM_017317674.1 | 44.6% | 45.8% | (many diffs) |
| 13 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | XM_017317675.1 | 42.7% | 44.2% | (many diffs) |
| 14 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | XM_006524989.3 | 41.7% | 42.8% | (many diffs) |
| 15 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | XM_011246654.2 | 41.7% | 42.8% | (many diffs) |
| 16 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | NM_028476.4 | 41.4% | 42.9% | (many diffs) |
| 17 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | XM_017317672.1 | 40.6% | 41.7% | (many diffs) |
| 18 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | NM_001142744.1 | 37.4% | 38.4% | (many diffs) |
| 19 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | NM_001347378.1 | 32.7% | 33.9% | (many diffs) |
| 20 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | XR_001782121.1 | 12.2% | (many diffs) | |
| 21 | mouse | 73242 | Atat1 | alpha tubulin acetyltransfe... | XR_001782120.1 | 11.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 603
- ORF length:
- 537
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gagtaaccgc catgttgttt atattctcaa agacagttca gcccgaccgg 121 ctggaaaagg agccattatt ggtttcatca aagttggata caagaagctc tttgtactgg 181 attatcgtga ggctcataat gaggtagaac cacttTGCAT CCTGGACTTT TACATCCATG 241 AGTCTGTGCA ACGCCATGGC CATGGGCGAG AACTCTTCCA GTATATGTTG CAGAAGGAGC 301 GAGTGGAACC GCACCAACTG GCAATTGACC GACCCTCACA GAAGCTGCTG AAATTCCTGA 361 ATAAGCACTA CAATCTGGAG ACCACAGTCC CACAGGTGAA CAACTTTGTG ATCTTTGAAG 421 GCTTCTTTGC CCATCAACAT CGGCCCCCTG CTCCCTCTCT GAGGGCAACT CGACACTCTC 481 GTGCTGCTGC AGTCGATCCC ACGCCCGCTG CTCCAGCAAG GAAGCTGCCA CCCAAGAGAG 541 CAGAGGGAGA CATCAAGCCA TACTCCTCTA GTGACCGAGA ATGGGGACTC CCCCAGGTCT 601 GGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 661 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 721 GGCTTTATAT ATCTTGTGGA AAGGACGACA CCAGTATCGG GACTCGTTCT GCACGCGTTA 781 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt