Construct: ORF TRCN0000466796
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008923.1_s317c1
- Derived from:
- ccsbBroadEn_09290
- DNA Barcode:
- CTTACAATAACTAAGCAGCCCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX21 (90203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466796
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90203 | SNX21 | sorting nexin family member 21 | NM_152897.3 | 99.6% | 99.4% | 460G>A;594T>C |
| 2 | human | 90203 | SNX21 | sorting nexin family member 21 | XM_011529096.2 | 83.3% | 73.7% | (many diffs) |
| 3 | human | 90203 | SNX21 | sorting nexin family member 21 | XM_011529098.2 | 82% | 76.3% | (many diffs) |
| 4 | human | 90203 | SNX21 | sorting nexin family member 21 | NM_001042632.3 | 76.2% | 74.3% | 440_441insGTACGTG;453G>A;456_457ins134 |
| 5 | human | 90203 | SNX21 | sorting nexin family member 21 | NM_001042633.3 | 75.9% | 75.3% | 447_457del;471G>A;474_475ins134 |
| 6 | human | 90203 | SNX21 | sorting nexin family member 21 | NM_033421.4 | 52.5% | 52.2% | (many diffs) |
| 7 | human | 90203 | SNX21 | sorting nexin family member 21 | XM_011529095.2 | 12.9% | 12.3% | (many diffs) |
| 8 | mouse | 101113 | Snx21 | sorting nexin family member 21 | NM_133924.3 | 44.4% | 45.8% | (many diffs) |
| 9 | mouse | 101113 | Snx21 | sorting nexin family member 21 | XM_006498528.3 | 43.7% | 44.9% | (many diffs) |
| 10 | mouse | 101113 | Snx21 | sorting nexin family member 21 | XM_006498527.3 | 43.4% | 44.7% | (many diffs) |
| 11 | mouse | 101113 | Snx21 | sorting nexin family member 21 | XM_006498529.3 | 14.6% | 12.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ccgtgggacg caggagggtg ccatggcctc ccggctcctg caccggctgc 121 ggcacgcctt ggccggcgac ggccccgggg aggcggcggc cagtccagag gccgagcagt 181 ttccggagag ctcagagctg gaggacgacg acgccgaggg cctgtcctcc cgactcagcg 241 gcacccTCAG CTTCACCAGC GCCGAGGACG ACGAGGACGA CGAGGACGAG GACGACGAGG 301 AGGCTGGCCC TGACCAGCTG CCCCTCGGGG ATGGGACGTC AGGAGAAGAC GCAGAACGGA 361 GCCCCCCACC TGATGGGCAG TGGGGCAGTC AGCTCCTGGC GCGGCAGCTG CAGGATTTCT 421 GGAAGAAGTC CCGGAACACC TTGGCACCCC AGCGGCTGCT CTTCGAAGTG ACCAGCGCTA 481 ACGTTGTCAA GGACCCGCCC TCCAAGTACG TGCTCTACAC CCTCACCGTG ATCGGCCCAG 541 GACCGCCAGA TTGCCAGCCA GCCCAGATCT CTCGCCGTTA CTCGGACTTT GAGCGGCTGC 601 ACCGAAACCT GCAGCGGCAA TTCCGGGGCC CAATGGCTGC CATCTCCTTC CCCCAGTCCC 661 ACTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGACT TACAATAACT AAGCAGCCCC CGACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt