Construct: ORF TRCN0000466912
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018516.1_s317c1
- Derived from:
- ccsbBroadEn_06672
- DNA Barcode:
- TAACGGTAGTCAGATCAGTGCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OPCML (4978)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466912
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4978 | OPCML | opioid binding protein/cell... | NM_002545.4 | 99.9% | 99.7% | 347C>A |
| 2 | human | 4978 | OPCML | opioid binding protein/cell... | NM_001319106.1 | 99.6% | 99.4% | 347C>A;525_526insGAA |
| 3 | human | 4978 | OPCML | opioid binding protein/cell... | NM_001319103.1 | 97.3% | 97.1% | 347C>A;937_963del |
| 4 | human | 4978 | OPCML | opioid binding protein/cell... | NM_001012393.3 | 94.9% | 93% | (many diffs) |
| 5 | human | 4978 | OPCML | opioid binding protein/cell... | XM_006718846.3 | 94.6% | 92.7% | (many diffs) |
| 6 | human | 4978 | OPCML | opioid binding protein/cell... | NM_001319105.1 | 88% | 87.8% | 0_1ins123;224C>A |
| 7 | human | 4978 | OPCML | opioid binding protein/cell... | XM_011542856.3 | 88% | 87.8% | 0_1ins123;224C>A |
| 8 | human | 4978 | OPCML | opioid binding protein/cell... | NM_001319104.2 | 70.9% | 70.7% | 0_1ins300;47C>A |
| 9 | mouse | 330908 | Opcml | opioid binding protein/cell... | XM_006510437.3 | 91.9% | 98.2% | (many diffs) |
| 10 | mouse | 330908 | Opcml | opioid binding protein/cell... | XM_006510438.3 | 91.6% | 97.9% | (many diffs) |
| 11 | mouse | 330908 | Opcml | opioid binding protein/cell... | XM_006510434.3 | 89.6% | 95.7% | (many diffs) |
| 12 | mouse | 330908 | Opcml | opioid binding protein/cell... | XM_006510435.3 | 89.3% | 95.4% | (many diffs) |
| 13 | mouse | 330908 | Opcml | opioid binding protein/cell... | NM_177906.4 | 86.8% | 91.3% | (many diffs) |
| 14 | mouse | 330908 | Opcml | opioid binding protein/cell... | XM_011242559.2 | 84.9% | 89.2% | (many diffs) |
| 15 | mouse | 330908 | Opcml | opioid binding protein/cell... | XM_006510439.3 | 78.3% | 84.1% | (many diffs) |
| 16 | mouse | 330908 | Opcml | opioid binding protein/cell... | XM_006510440.2 | 62.9% | 67.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1104
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggtctgt gggtacctgt tcctgccctg gaagtgcctc gtggtcgtgt 121 ctctcaggct gctgttcctt gtacccacag gagtgcccgt gcgcagcgga gatgccacct 181 tccccaaagc tatggacaac gtgacggtcc ggcaggggga gagcgccacc ctcaggtgta 241 ccatagatga ccgggtaacc cgggtggcct ggctaaaccg cagcaccatc ctctacgctg 301 ggaatgacaa gtggtccata gaccctcgtg tgatcatcct ggtcaataca ccaacccagt 361 acagcatcat gatccaaaat gtggatgtgt atgacgaagg tccgtacacc tgctatgtgc 421 agacagacaa tcatcccaaa acgtcccggg ttcacctaat agtgcaagtt cctcctcaga 481 tcatgaatat ctcctcagac atcactgtga atgagggaag cagtgtgacc ctgctgtgtc 541 ttgctattgg cagaccagag ccaactgtga catggagaca cctgtcagtc aaggaaggcc 601 agggctttgt aagtgaggat gagtacctgg agatctctga catcaagcga gaccagtccg 661 gggagtacga atgcagcgcg ttgaacgatg tcgctgcgcc cgatgtgcgg aaagtaaaaa 721 tcactgtaaa ctatcctccc tatatctcaa aagccaagaa cactggtgtt tcagtcggtc 781 agaagggcat ccTGAGCTGT GAAGCCTCTG CAGTCCCCAT GGCTGAATTC CAGTGGTTCA 841 AGGAAGAAAC CAGGTTAGCC ACTGGTCTGG ATGGAATGAG GATTGAAAAC AAAGGCCGCA 901 TGTCCACTCT GACTTTCTTC AATGTTTCAG AAAAGGATTA TGGGAACTAT ACTTGTGTGG 961 CCACGAACAA GCTTGGGAAC ACCAATGCCA GCATCACATT GTATGGGCCT GGAGCAGTCA 1021 TTGATGGTGT AAACTCGGCC TCCAGAGCAC TGGCTTGTCT CTGGCTATCA GGGACCCTCT 1081 TAGCCCACTT CTTCATCAAG TTTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1141 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1201 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AACGGTAGTC 1261 AGATCAGTGC ATCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1321 att