Transcript: Mouse XM_006510438.3

PREDICTED: Mus musculus opioid binding protein/cell adhesion molecule-like (Opcml), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Opcml (330908)
Length:
7378
CDS:
1121..2155

Additional Resources:

NCBI RefSeq record:
XM_006510438.3
NBCI Gene record:
Opcml (330908)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006510438.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000113637 GCCTACCCAATACAGTATCAT pLKO.1 1402 CDS 100% 5.625 7.875 N Opcml n/a
2 TRCN0000113636 CCGCATATCCACTTTGACTTT pLKO.1 1945 CDS 100% 4.950 6.930 N Opcml n/a
3 TRCN0000113639 GAAGCAGTGTGACCCTGTTAT pLKO.1 1569 CDS 100% 13.200 9.240 N Opcml n/a
4 TRCN0000113635 GCTGCCTAACTGCATCCTTAA pLKO.1 2750 3UTR 100% 10.800 7.560 N Opcml n/a
5 TRCN0000113638 GCACCTGATGTTCGGAAAGTA pLKO.1 1745 CDS 100% 5.625 3.938 N Opcml n/a
6 TRCN0000166364 CACACACACACACACACACAA pLKO.1 2504 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006510438.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11009 pDONR223 100% 91.9% 98.2% None (many diffs) n/a
2 ccsbBroad304_11009 pLX_304 0% 91.9% 98.2% V5 (many diffs) n/a
3 TRCN0000481031 TTCTTCCCATCAGATCGGTGATAT pLX_317 40.9% 91.9% 98.2% V5 (many diffs) n/a
4 TRCN0000488056 CACGACTAATCCAGAACAAGACAC pLX_317 30.2% 91.7% 98.2% V5 (many diffs) n/a
5 TRCN0000488347 AGTGCCATTTAGCATCCTCTTTTT pLX_317 30.5% 91.7% 98.2% V5 (not translated due to prior stop codon) (many diffs) n/a
6 ccsbBroadEn_06672 pDONR223 100% 91.6% 97.9% None (many diffs) n/a
7 ccsbBroad304_06672 pLX_304 0% 91.6% 97.9% V5 (many diffs) n/a
8 TRCN0000466912 TAACGGTAGTCAGATCAGTGCATC pLX_317 30.4% 91.6% 97.9% V5 (many diffs) n/a
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