Construct: ORF TRCN0000466954
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003681.1_s317c1
- Derived from:
- ccsbBroadEn_07685
- DNA Barcode:
- CGCCCAAGTATTAATTACCCAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEPTIN9 (10801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466954
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10801 | SEPTIN9 | septin 9 | NM_006640.4 | 99.8% | 99.6% | 1672A>G;1703T>G |
| 2 | human | 10801 | SEPTIN9 | septin 9 | NM_001293695.2 | 99.1% | 98.5% | (many diffs) |
| 3 | human | 10801 | SEPTIN9 | septin 9 | NM_001113493.2 | 97.5% | 96.8% | (many diffs) |
| 4 | human | 10801 | SEPTIN9 | septin 9 | NM_001113491.2 | 96.6% | 95.3% | (many diffs) |
| 5 | human | 10801 | SEPTIN9 | septin 9 | NM_001113492.1 | 74.1% | 73.9% | 0_1ins438;1234A>G;1265T>G |
| 6 | human | 10801 | SEPTIN9 | septin 9 | NM_001113494.1 | 74.1% | 73.9% | 0_1ins438;1234A>G;1265T>G |
| 7 | human | 10801 | SEPTIN9 | septin 9 | NM_001113495.1 | 72.5% | 55.2% | (many diffs) |
| 8 | human | 10801 | SEPTIN9 | septin 9 | NM_001293696.2 | 62.4% | 60.2% | (many diffs) |
| 9 | human | 10801 | SEPTIN9 | septin 9 | NM_001113496.2 | 58.8% | 58.6% | 0_1ins699;973A>G;1004T>G |
| 10 | human | 10801 | SEPTIN9 | septin 9 | NM_001293697.2 | 58.8% | 58.6% | 0_1ins699;973A>G;1004T>G |
| 11 | human | 10801 | SEPTIN9 | septin 9 | NM_001293698.2 | 58.8% | 58.6% | 0_1ins699;973A>G;1004T>G |
| 12 | mouse | 53860 | Sept9 | septin 9 | XM_006533744.1 | 85.3% | 89.9% | (many diffs) |
| 13 | mouse | 53860 | Sept9 | septin 9 | NM_001113487.1 | 83.7% | 87.5% | (many diffs) |
| 14 | mouse | 53860 | Sept9 | septin 9 | NM_001113486.1 | 82.8% | 86.2% | (many diffs) |
| 15 | mouse | 53860 | Sept9 | septin 9 | NM_001113488.1 | 52.6% | 56.8% | (many diffs) |
| 16 | mouse | 53860 | Sept9 | septin 9 | NM_017380.2 | 52.6% | 56.8% | (many diffs) |
| 17 | mouse | 53860 | Sept9 | septin 9 | XM_006533745.3 | 52.6% | 56.8% | (many diffs) |
| 18 | mouse | 53860 | Sept9 | septin 9 | XM_006533746.3 | 52.6% | 56.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1770
- ORF length:
- 1704
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gagggaccgg atctcagcct tgaaaagatc ttttgaggtc gaggaggtcg 121 agacacccaa ctccacccca ccccggaggg tccagactcc cctactccga gccactgtgg 181 ccagctccac ccagaaattc caggacctgg gcgtgaagaa ctcagaaccc tcggcccgcc 241 atgtggactc cctaagccaa cgctccccca aggcgtccct gcggagggtg gagctctcgg 301 gccccaaggc ggccgagccg gtgtcccggc gcactgagct gtccattgac atctcgtcca 361 agcaggtgga gaacgccggg gccatcggcc cgtcccggtt cgggctcaag agggccgagg 421 tgttgggcca caagacgcca gaaccggccc ctcggaggac ggagatcacc atcgtcaaac 481 cccaggagtc agcccaccgg aggatggagc cccctgcctc caaggtcccc gaggtgccca 541 ctgcccctgc caccgacgca gcccccaaga gggtggagat ccagatgccc aagcctgctg 601 aggcgcccac cgcccccagc ccagcccaga ccttggagaa ttcagagcct gcccctgtgt 661 ctcagctgca gagcaggctg gagcccaagc cccagccccc tgtggctgag gctacacccc 721 ggagccagga ggccactgag gcggctccca gctgcgttgg cgacatggcc gacaccccca 781 gagatgccgg gctcaagcag gcgcctgcat cacggaacga gaaggccccg gtggacttcg 841 gctacgtggg gattgactcc atcctggagc agatgcgccg gaaggccatg aagcagggct 901 tcgagttcaa catcatggtg gtcgggcaga gcggcttggg taaatccacc ttaatcaaca 961 ccctcttcaa atccaaaatc agccggaagt cggtgcagcc cacctcagag gagcgcatcc 1021 ccaagaccat cgagatcaag tccatcacgc acgatattga ggagaaaggc gtccggatga 1081 agctgacagt gattgacaca ccagggttcg gggaccacat caacaacgag aactgctggc 1141 agcccatcat gaagttcatc aatgaccagt acgagaaata cctgcaggag gaggtcaaca 1201 tcaaccgcaa gaagcgcatc ccggacaccc gcgtccactg ctgcctctac ttcatccccg 1261 ccaccggcca ctccctcagg cccctggaca tcgagtttat gaaacgcctg agcaaggtgg 1321 tcaacatcgt ccctgtcatc gccaaggcgg acacactcac cctggaggag agggtccact 1381 tcaaacagcg gatcaccgca gaccTGCTGT CCAACGGCAT CGACGTGTAC CCCCAGAAGG 1441 AATTTGATGA GGACTCGGAG GACCGGCTGG TGAACGAGAA GTTCCGGGAG ATGATCCCAT 1501 TTGCTGTGGT GGGCAGTGAC CACGAGTACC AGGTCAACGG CAAGAGGATC CTTGGGAGGA 1561 AGACCAAGTG GGGTACCATC GAAGTTGAAA ACACCACACA CTGTGAGTTT GCCTACCTGC 1621 GGGACCTTCT CATCAGGACG CACATGCAGA ACATCAAGGA CATCACCAGC AGCATCCACT 1681 TCGAGGCGTA CCGTGTGAAG CGCCTCAACG AGGGCAGCAG CGCCATGGCC AACGGCGTGG 1741 AGGAGAAGGA GCCAGAAGCC CCGGAGAGGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1801 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1861 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGCCC 1921 AAGTATTAAT TACCCAGTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1981 tgaaagatt