Construct: ORF TRCN0000466954

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003681.1_s317c1
Derived from:
ccsbBroadEn_07685
DNA Barcode:
CGCCCAAGTATTAATTACCCAGTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEPTIN9 (10801)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466954
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10801 SEPTIN9 septin 9 NM_006640.4 99.8% 99.6% 1672A>G;1703T>G
2 human 10801 SEPTIN9 septin 9 NM_001293695.2 99.1% 98.5% (many diffs)
3 human 10801 SEPTIN9 septin 9 NM_001113493.2 97.5% 96.8% (many diffs)
4 human 10801 SEPTIN9 septin 9 NM_001113491.2 96.6% 95.3% (many diffs)
5 human 10801 SEPTIN9 septin 9 NM_001113492.1 74.1% 73.9% 0_1ins438;1234A>G;1265T>G
6 human 10801 SEPTIN9 septin 9 NM_001113494.1 74.1% 73.9% 0_1ins438;1234A>G;1265T>G
7 human 10801 SEPTIN9 septin 9 NM_001113495.1 72.5% 55.2% (many diffs)
8 human 10801 SEPTIN9 septin 9 NM_001293696.2 62.4% 60.2% (many diffs)
9 human 10801 SEPTIN9 septin 9 NM_001113496.2 58.8% 58.6% 0_1ins699;973A>G;1004T>G
10 human 10801 SEPTIN9 septin 9 NM_001293697.2 58.8% 58.6% 0_1ins699;973A>G;1004T>G
11 human 10801 SEPTIN9 septin 9 NM_001293698.2 58.8% 58.6% 0_1ins699;973A>G;1004T>G
12 mouse 53860 Sept9 septin 9 XM_006533744.1 85.3% 89.9% (many diffs)
13 mouse 53860 Sept9 septin 9 NM_001113487.1 83.7% 87.5% (many diffs)
14 mouse 53860 Sept9 septin 9 NM_001113486.1 82.8% 86.2% (many diffs)
15 mouse 53860 Sept9 septin 9 NM_001113488.1 52.6% 56.8% (many diffs)
16 mouse 53860 Sept9 septin 9 NM_017380.2 52.6% 56.8% (many diffs)
17 mouse 53860 Sept9 septin 9 XM_006533745.3 52.6% 56.8% (many diffs)
18 mouse 53860 Sept9 septin 9 XM_006533746.3 52.6% 56.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1770
ORF length:
1704
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gagggaccgg atctcagcct tgaaaagatc ttttgaggtc gaggaggtcg
121agacacccaa ctccacccca ccccggaggg tccagactcc cctactccga gccactgtgg
181ccagctccac ccagaaattc caggacctgg gcgtgaagaa ctcagaaccc tcggcccgcc
241atgtggactc cctaagccaa cgctccccca aggcgtccct gcggagggtg gagctctcgg
301gccccaaggc ggccgagccg gtgtcccggc gcactgagct gtccattgac atctcgtcca
361agcaggtgga gaacgccggg gccatcggcc cgtcccggtt cgggctcaag agggccgagg
421tgttgggcca caagacgcca gaaccggccc ctcggaggac ggagatcacc atcgtcaaac
481cccaggagtc agcccaccgg aggatggagc cccctgcctc caaggtcccc gaggtgccca
541ctgcccctgc caccgacgca gcccccaaga gggtggagat ccagatgccc aagcctgctg
601aggcgcccac cgcccccagc ccagcccaga ccttggagaa ttcagagcct gcccctgtgt
661ctcagctgca gagcaggctg gagcccaagc cccagccccc tgtggctgag gctacacccc
721ggagccagga ggccactgag gcggctccca gctgcgttgg cgacatggcc gacaccccca
781gagatgccgg gctcaagcag gcgcctgcat cacggaacga gaaggccccg gtggacttcg
841gctacgtggg gattgactcc atcctggagc agatgcgccg gaaggccatg aagcagggct
901tcgagttcaa catcatggtg gtcgggcaga gcggcttggg taaatccacc ttaatcaaca
961ccctcttcaa atccaaaatc agccggaagt cggtgcagcc cacctcagag gagcgcatcc
1021ccaagaccat cgagatcaag tccatcacgc acgatattga ggagaaaggc gtccggatga
1081agctgacagt gattgacaca ccagggttcg gggaccacat caacaacgag aactgctggc
1141agcccatcat gaagttcatc aatgaccagt acgagaaata cctgcaggag gaggtcaaca
1201tcaaccgcaa gaagcgcatc ccggacaccc gcgtccactg ctgcctctac ttcatccccg
1261ccaccggcca ctccctcagg cccctggaca tcgagtttat gaaacgcctg agcaaggtgg
1321tcaacatcgt ccctgtcatc gccaaggcgg acacactcac cctggaggag agggtccact
1381tcaaacagcg gatcaccgca gaccTGCTGT CCAACGGCAT CGACGTGTAC CCCCAGAAGG
1441AATTTGATGA GGACTCGGAG GACCGGCTGG TGAACGAGAA GTTCCGGGAG ATGATCCCAT
1501TTGCTGTGGT GGGCAGTGAC CACGAGTACC AGGTCAACGG CAAGAGGATC CTTGGGAGGA
1561AGACCAAGTG GGGTACCATC GAAGTTGAAA ACACCACACA CTGTGAGTTT GCCTACCTGC
1621GGGACCTTCT CATCAGGACG CACATGCAGA ACATCAAGGA CATCACCAGC AGCATCCACT
1681TCGAGGCGTA CCGTGTGAAG CGCCTCAACG AGGGCAGCAG CGCCATGGCC AACGGCGTGG
1741AGGAGAAGGA GCCAGAAGCC CCGGAGAGGT ACCCAACTTT CTTGTACAAA GTGGTTGATA
1801TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1861CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGCCC
1921AAGTATTAAT TACCCAGTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1981tgaaagatt

Download FASTA (ORF) (Full)