Construct: ORF TRCN0000466984
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007982.1_s317c1
- Derived from:
- ccsbBroadEn_06516
- DNA Barcode:
- AACCGCTAGTGGTACGGCGCCTCA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LASP1 (3927)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466984
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3927 | LASP1 | LIM and SH3 protein 1 | NM_006148.4 | 99.6% | 100% | 357C>T;438A>G;783C>T |
| 2 | human | 3927 | LASP1 | LIM and SH3 protein 1 | NM_001271608.1 | 78.1% | 68.6% | (many diffs) |
| 3 | human | 3927 | LASP1 | LIM and SH3 protein 1 | NR_073384.2 | 19.1% | (many diffs) | |
| 4 | mouse | 16796 | Lasp1 | LIM and SH3 protein 1 | NM_010688.4 | 90.3% | 95.4% | (many diffs) |
| 5 | mouse | 16796 | Lasp1 | LIM and SH3 protein 1 | XM_006532341.1 | 90.3% | 95.4% | (many diffs) |
| 6 | mouse | 16796 | Lasp1 | LIM and SH3 protein 1 | XM_006532342.1 | 77.4% | 82.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 849
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccccaactgc gcccggtgcg gcaagatcgt gtatcccacg gagaaggtga 121 actgtctgga taagttctgg cataaagcat gcttccattg cgagacctgc aagatgacac 181 tgaacatgaa gaactacaag ggctacgaga agaagcccta ctgcaacgca cactacccca 241 agcagtcctt caccatggtg gcggacaccc cggaaaacct tcgcctcaag caacagagtg 301 agctccagag tcaggtgcgc tacaaggagg agtttgagaa gaacaagggc aaaggtttca 361 gcgtagtggc agacacgccc gagctccaga gaaTCAAGAA GACCCAGGAC CAGATCAGTA 421 ATATAAAATA CCATGAGGAG TTTGAGAAGA GCCGCATGGG CCCTAGCGGG GGCGAGGGCA 481 TGGAGCCAGA GCGTCGGGAT TCGCAGGACG GCAGCAGCTA CCGGCGGCCC CTGGAGCAGC 541 AGCAGCCTCA CCACATCCCG ACCAGTGCCC CGGTTTACCA GCAGCCCCAG CAGCAGCCGG 601 TGGCCCAGTC CTATGGTGGC TACAAGGAGC CTGCAGCCCC AGTCTCCATA CAGCGCAGCG 661 CCCCAGGTGG TGGCGGGAAG CGGTACCGCG CGGTGTATGA CTACAGCGCC GCCGACGAGG 721 ACGAGGTCTC CTTCCAGGAC GGGGACACCA TCGTCAACGT GCAGCAGATC GACGACGGCT 781 GGATGTACGG GACGGTGGAG CGCACCGGCG ACACGGGGAT GCTGCCGGCC AACTACGTGG 841 AGGCCATTTG CCCAACTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 901 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 961 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAACCGCT AGTGGTACGG CGCCTCAACG 1021 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt