Construct: ORF TRCN0000466991

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002612.1_s317c1
Derived from:
ccsbBroadEn_08533
DNA Barcode:
CCCTGTGGATACATCTAACCATGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AP1AR (55435)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466991
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55435 AP1AR adaptor related protein com... NM_001128426.3 99.6% 99.2% 15C>G;628A>G;807A>G
2 human 55435 AP1AR adaptor related protein com... NM_018569.6 88.7% 88.4% (many diffs)
3 human 55435 AP1AR adaptor related protein com... XM_017008379.2 76.8% 76.9% (many diffs)
4 human 55435 AP1AR adaptor related protein com... XM_017008378.2 68.5% 68.5% (many diffs)
5 human 55435 AP1AR adaptor related protein com... XM_017008380.1 52.5% 52.4% 0_1ins381;247A>G;426A>G
6 human 55435 AP1AR adaptor related protein com... NR_136622.2 13.6% (many diffs)
7 mouse 211556 Ap1ar adaptor-related protein com... NM_001346718.1 82.3% 88.4% (many diffs)
8 mouse 211556 Ap1ar adaptor-related protein com... NM_145964.2 73.6% 79.1% (many diffs)
9 mouse 211556 Ap1ar adaptor-related protein com... XM_006501244.2 70.8% 66.8% (many diffs)
10 mouse 211556 Ap1ar adaptor-related protein com... XM_006501245.2 68.4% 72.6% (many diffs)
11 mouse 211556 Ap1ar adaptor-related protein com... XM_006501243.2 66.6% 64.2% (many diffs)
12 mouse 211556 Ap1ar adaptor-related protein com... XM_006501242.1 65.4% 61.6% (many diffs)
13 mouse 211556 Ap1ar adaptor-related protein com... XM_006501241.2 64.2% 60.5% (many diffs)
14 mouse 211556 Ap1ar adaptor-related protein com... XM_006501240.1 59.9% 56.6% (many diffs)
15 mouse 211556 Ap1ar adaptor-related protein com... XM_006501247.2 56.4% 60.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
873
ORF length:
807
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gaactgctgg tggacgcagt gcttcggact gcttcgcaag gaagcggggc
121ggctgcagcg agtaggcggc ggcggaggat ccaagtattt tagaacatgc tcaagaggtg
181agcacttaac aatagagttt gagaatctag tagaaagtga tgaaggggag agcccaggaa
241gcagtcatag gcctcttact gaggaagaaa ttgttgacct aagagaaagg cattatgatt
301ccattgccga aaaacaaaaa gatcttgata agaaaattca aaaagagcaa gaaaggcaga
361gaattgtgca gcaatatcat ccttccaaca atGGAGAATA TCAAAGTTCA GGACCAGAAG
421ATGACTTCGA ATCTTGTTTG AGAAATATGA AGTCACAGTA TGAAGTTTTT CGAAGTAGTA
481GACTCTCATC AGATGCTACA GTTTTGACAC CAAATACAGA AAGCAGTTGT GATTTAATGA
541CCAAAACTAA ATCAACTAGT GGAAATGACG ACAGCACATC CTTAGATCTA GAGTGGGAAG
601ATGAAGAAGG AATGAATAGA ATGCTTCCAA TGAGAGAACG TTCCAAAACA GAGGAAGACA
661TTCTACGGGC AGCACTTAAG TATAGCAACA AGGAGACTGG AAGTAATCCT ACATCAGCCT
721CTGATGATTC CAATGGGCTG GAGTGGGAAA ATGATTTTGT TAGTGCCGAA ATGGATGATA
781ATGGAAATTC CGAGTATTCT GGATTTGTAA ATCCTGTATT AGAACTGTCT GATTCTGGCA
841TAAGGCATTC TGACACAGAT CAACAGACTC GGTACCCAAC TTTCTTGTAC AAAGTGGTTG
901ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
961GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC
1021CTGTGGATAC ATCTAACCAT GCACGCGTTA AGTCgacaat caacctctgg attacaaaat
1081ttgtgaaaga tt

Download FASTA (ORF) (Full)