Construct: ORF TRCN0000467035
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005543.1_s317c1
- Derived from:
- ccsbBroadEn_15579
- DNA Barcode:
- TAGACGGCAAACCGAACAATATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS4Y1 (6192)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467035
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6192 | RPS4Y1 | ribosomal protein S4 Y-link... | NM_001008.4 | 100% | 100% | |
| 2 | human | 140032 | RPS4Y2 | ribosomal protein S4 Y-link... | NM_001039567.3 | 93.5% | 93.9% | (many diffs) |
| 3 | human | 6191 | RPS4X | ribosomal protein S4 X-linked | NM_001007.5 | 82.1% | 92.7% | (many diffs) |
| 4 | mouse | 20102 | Rps4x | ribosomal protein S4, X-linked | NM_009094.2 | 80.9% | 92.7% | (many diffs) |
| 5 | mouse | 20102 | Rps4x | ribosomal protein S4, X-linked | XM_011247553.2 | 75.5% | 85.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 855
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccggggcccc aagaagcact taaagcgtgt tgcagcgccg aagcattgga 121 tgcttgacaa actaacgggt gtatttgcac ctcgtccatc gacaggtccc cacaagctga 181 gggaatgtct tcctctgatc gtcttcctca ggaatagact caagtatgcg ttgactggag 241 atgaggtaaa gaagatatgt atgcaacgtt tcatcaaaat tgatggcaag gttcgagtgg 301 atgtcacata ccctgctgga ttcatggatg tcatcagcat cgagaagaca ggtgaacatt 361 tccgcctggt ctatgacacc aagggccgtt ttgctgttca ccgcatcaca gtggaagagg 421 caaagtacaa gttgtgcaaa gtgaggaaga ttactgtggg agtgaaggga atccctcacc 481 tggtgactca tgatgctcga accatccgct acccagatcc tgtcatcaag gtgaacgata 541 ctgtgcagat tgatttaggg actggcaaga taaTCAACTT TATCAAATTT GATACAGGCA 601 ATTTGTGTAT GGTGATTGGT GGAGCCAACC TCGGTCGTGT TGGTGTGATC ACCAACAGGG 661 AAAGACATCC TGGTTCTTTT GATGTGGTGC ATGTGAAGGA TGCCAATGGC AACAGCTTTG 721 CCACGAGGCT TTCCAACATT TTTGTCATTG GCAATGGCAA TAAACCTTGG ATTTCCCTGC 781 CCAGGGGAAA GGGCATTCGA CTTACTGTTG CTGAAGAGAG AGATAAGAGG CTGGCCACCA 841 AACAGAGCAG TGGCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 901 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 961 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TAGACGGCAA ACCGAACAAT 1021 ATTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt