Construct: ORF TRCN0000467147

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018921.3_s317c1
Derived from:
ccsbBroadEn_15036
DNA Barcode:
ATCACCGGTGCAAGCGATCAAGAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
LATS2 (26524)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467147
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26524 LATS2 large tumor suppressor kina... NM_014572.3 46.1% 23.1% (many diffs)
2 human 26524 LATS2 large tumor suppressor kina... XM_005266342.1 46.1% 23.1% (many diffs)
3 human 26524 LATS2 large tumor suppressor kina... XM_017020541.1 44.4% 12.7% (many diffs)
4 human 26524 LATS2 large tumor suppressor kina... XM_011535042.2 43.5% 13% (many diffs)
5 human 26524 LATS2 large tumor suppressor kina... XM_017020542.1 29.4% 27.8% (many diffs)
6 mouse 50523 Lats2 large tumor suppressor 2 NM_015771.2 40.6% 22.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
894
ORF length:
825
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaggccaaag acttttcctg ccacgactta ttctggaaat agccggcagc
121gactgcaaga gattcgtgag gggttaaagc agccatccaa gtcttcggtt caggggctac
181ccgcaggacc aaacagtgac acttccctgg atgccaaagt cctggggagc aaagatgcca
241ccaggcagca gcagcagatg agagccaccc caaagttcgg accttatcag aaagccttga
301gggaaatcag atattccttg ttgccttttg ctaatgaatc gggcacctct gcagctgcag
361aagtgaaccg gcaaatgctg caggaactgg tgaacgcagg atgcgaccag gagatggctg
421gccgagctct caagcagact ggcagcagga gcatcgaggc cgccctggag tacatcagca
481agatgggcta cctggacccg aggaatgagc agattgtgcg ggtcattaag cagacctccc
541caggaaaggg gctcatgcca accccagtga cgcggaggcc cagcttcgaa ggaaccggcg
601attcgtttgc gtccttccct gagcacctgg cccggttcta catcgcagag ctgactttgg
661ccattgagag tgtccacaag atgggcttca tccaccgaga catcaagcct gataacattt
721tgatagatct ggatggtcac attaaactca cagatttcgg cctctgcact gggttcagtg
781actcacaant cccaaantat tnccagaaan gnagccatgt cagacaggac agcatggagc
841ccagcgacnt tctggggatg atgtggttct ancnggtcgg tgtggggaca ggctgagacc
901cctagagcag agggcgcgga agcagcacca gaggtgcctg gcacattcac tggtggggac
961tccaaactac atcgcacccg aggtgctcct ccgcaaaggg tacactcaac tctgtgactg
1021gtggagtgtt ggagtgattc tcttcgagat gctggtgggg cagccgccct ttttggcacc
1081tactcccaca gaaacccagc tgaaggtgat caactgggag aacacgctcc acattccagc
1141ccaggtgaag ctgagccctg aggccaggga cctcatcacc aagctgtgct gctccgcaga
1201ccaccgcctg gggcggaatg gggccgatga cctgaaggcc caccccTTCT TCAGCGCCAT
1261TGACTTCTCC AGTGACATCC GGAAGCAGCC AGCCCCCTAC GTTCCCACCA TCAGCCACCC
1321CATGGACACC TCGAATTTCG ACCCCGTAGA TGAAGAAAGC CCTTGGAACG ATGCCAGCGA
1381AGGTAGCACC AAGGCCTGGG ACACACTCAC CTCGCCCAAT AACAAGCATC CTGAGCACGC
1441ATTTTACGAA TTCACCTTCC GAAGGTTCTT TGATGACAAT GGCTACCCCT TTCGATGCCC
1501AAAGCCTTCA GGAGCAGAAG CTTCACAGGC TGAGAGCTCA GATTTAGAAA GCTCTGATCT
1561GGTGGATCAG ACTGAAGGCT GCCAGCCTGT GTACGTGTTG CCAACTTTCT TGTACAAAGT
1621GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG
1681AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA
1741CGAATCACCG GTGCAAGCGA TCAAGATACG CGTTAAGTCg acaatcaacc tctggattac
1801aaaatttgtg aaagatt

Download FASTA (ORF) (Full)