Construct: ORF TRCN0000467147
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018921.3_s317c1
- Derived from:
- ccsbBroadEn_15036
- DNA Barcode:
- ATCACCGGTGCAAGCGATCAAGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LATS2 (26524)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467147
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26524 | LATS2 | large tumor suppressor kina... | NM_014572.3 | 46.1% | 23.1% | (many diffs) |
| 2 | human | 26524 | LATS2 | large tumor suppressor kina... | XM_005266342.1 | 46.1% | 23.1% | (many diffs) |
| 3 | human | 26524 | LATS2 | large tumor suppressor kina... | XM_017020541.1 | 44.4% | 12.7% | (many diffs) |
| 4 | human | 26524 | LATS2 | large tumor suppressor kina... | XM_011535042.2 | 43.5% | 13% | (many diffs) |
| 5 | human | 26524 | LATS2 | large tumor suppressor kina... | XM_017020542.1 | 29.4% | 27.8% | (many diffs) |
| 6 | mouse | 50523 | Lats2 | large tumor suppressor 2 | NM_015771.2 | 40.6% | 22.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 894
- ORF length:
- 825
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggccaaag acttttcctg ccacgactta ttctggaaat agccggcagc 121 gactgcaaga gattcgtgag gggttaaagc agccatccaa gtcttcggtt caggggctac 181 ccgcaggacc aaacagtgac acttccctgg atgccaaagt cctggggagc aaagatgcca 241 ccaggcagca gcagcagatg agagccaccc caaagttcgg accttatcag aaagccttga 301 gggaaatcag atattccttg ttgccttttg ctaatgaatc gggcacctct gcagctgcag 361 aagtgaaccg gcaaatgctg caggaactgg tgaacgcagg atgcgaccag gagatggctg 421 gccgagctct caagcagact ggcagcagga gcatcgaggc cgccctggag tacatcagca 481 agatgggcta cctggacccg aggaatgagc agattgtgcg ggtcattaag cagacctccc 541 caggaaaggg gctcatgcca accccagtga cgcggaggcc cagcttcgaa ggaaccggcg 601 attcgtttgc gtccttccct gagcacctgg cccggttcta catcgcagag ctgactttgg 661 ccattgagag tgtccacaag atgggcttca tccaccgaga catcaagcct gataacattt 721 tgatagatct ggatggtcac attaaactca cagatttcgg cctctgcact gggttcagtg 781 actcacaant cccaaantat tnccagaaan gnagccatgt cagacaggac agcatggagc 841 ccagcgacnt tctggggatg atgtggttct ancnggtcgg tgtggggaca ggctgagacc 901 cctagagcag agggcgcgga agcagcacca gaggtgcctg gcacattcac tggtggggac 961 tccaaactac atcgcacccg aggtgctcct ccgcaaaggg tacactcaac tctgtgactg 1021 gtggagtgtt ggagtgattc tcttcgagat gctggtgggg cagccgccct ttttggcacc 1081 tactcccaca gaaacccagc tgaaggtgat caactgggag aacacgctcc acattccagc 1141 ccaggtgaag ctgagccctg aggccaggga cctcatcacc aagctgtgct gctccgcaga 1201 ccaccgcctg gggcggaatg gggccgatga cctgaaggcc caccccTTCT TCAGCGCCAT 1261 TGACTTCTCC AGTGACATCC GGAAGCAGCC AGCCCCCTAC GTTCCCACCA TCAGCCACCC 1321 CATGGACACC TCGAATTTCG ACCCCGTAGA TGAAGAAAGC CCTTGGAACG ATGCCAGCGA 1381 AGGTAGCACC AAGGCCTGGG ACACACTCAC CTCGCCCAAT AACAAGCATC CTGAGCACGC 1441 ATTTTACGAA TTCACCTTCC GAAGGTTCTT TGATGACAAT GGCTACCCCT TTCGATGCCC 1501 AAAGCCTTCA GGAGCAGAAG CTTCACAGGC TGAGAGCTCA GATTTAGAAA GCTCTGATCT 1561 GGTGGATCAG ACTGAAGGCT GCCAGCCTGT GTACGTGTTG CCAACTTTCT TGTACAAAGT 1621 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 1681 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 1741 CGAATCACCG GTGCAAGCGA TCAAGATACG CGTTAAGTCg acaatcaacc tctggattac 1801 aaaatttgtg aaagatt