Transcript: Human NM_014572.3

Homo sapiens large tumor suppressor kinase 2 (LATS2), mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
LATS2 (26524)
Length:
5546
CDS:
446..3712

Additional Resources:

NCBI RefSeq record:
NM_014572.3
NBCI Gene record:
LATS2 (26524)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147222 ACCAGCAGAAGGTTAACCGG pXPR_003 AGG 1866 57% 4 0.7222 LATS2 LATS2 77900
2 BRDN0001487153 GTAGGACGCAAACGAATCGC pXPR_003 CGG 532 16% 4 0.5951 LATS2 LATS2 77901
3 BRDN0001145273 AAGACGCCGCCGGAGACCGG pXPR_003 GGG 848 26% 4 0.3383 LATS2 LATS2 77899
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_014572.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000884 CTACTCGCCATACGCCTTTAA pLKO.1 2233 CDS 100% 13.200 18.480 N LATS2 n/a
2 TRCN0000197058 GCAGATTGTGCGGGTCATTAA pLKO.1 886 CDS 100% 13.200 18.480 N LATS2 n/a
3 TRCN0000195674 CCCAAAGTTCGGACCTTATCA pLKO.1 646 CDS 100% 5.625 7.875 N LATS2 n/a
4 TRCN0000000880 CCGTCGATTACTTCACTTGAA pLKO.1 3821 3UTR 100% 4.950 6.930 N LATS2 n/a
5 TRCN0000199570 GCCATCCAAGTCTTCGGTTCA pLKO.1 529 CDS 100% 4.050 3.240 N LATS2 n/a
6 TRCN0000194755 CACTTGAAATTCTGCTCTTCA pLKO.1 3834 3UTR 100% 4.950 3.465 N LATS2 n/a
7 TRCN0000000882 CCTCTGGGATGATGTGTCTAA pLKO.1 2965 CDS 100% 4.950 3.465 N LATS2 n/a
8 TRCN0000000881 GCCATGAAGACCCTAAGGAAA pLKO.1 2528 CDS 100% 4.950 3.465 N LATS2 n/a
9 TRCN0000000883 CAGGACCAAACAGTGACACTT pLKO.1 561 CDS 100% 4.950 2.970 N LATS2 n/a
10 TRCN0000166364 CACACACACACACACACACAA pLKO.1 5048 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_014572.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491325 AAGCGTCCACGACTTTACTTCTCC pLX_317 8.8% 94.3% 94.3% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000488615 TCCCTGCAGGCGAGTTCCCTAGTC pLX_317 10% 94.2% 94.2% V5 (many diffs) n/a
3 ccsbBroadEn_15036 pDONR223 100% 46.1% 23.1% None (many diffs) n/a
4 ccsbBroad304_15036 pLX_304 0% 46.1% 23.1% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000467147 ATCACCGGTGCAAGCGATCAAGAT pLX_317 23.3% 46.1% 23.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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