Construct: ORF TRCN0000467159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012772.1_s317c1
- Derived from:
- ccsbBroadEn_00715
- DNA Barcode:
- AGTATACGATAATTTCTCCAAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H1-4 (3008)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467159
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3008 | H1-4 | H1.4 linker histone, cluste... | NM_005321.2 | 100% | 100% | |
| 2 | human | 3006 | H1-2 | H1.2 linker histone, cluste... | NM_005319.3 | 77.4% | 84.5% | (many diffs) |
| 3 | human | 3009 | H1-5 | H1.5 linker histone, cluste... | NM_005322.2 | 74.6% | 83.7% | (many diffs) |
| 4 | mouse | 50709 | Hist1h1e | histone cluster 1, H1e | NM_015787.4 | 86.1% | 94% | (many diffs) |
| 5 | mouse | 14957 | H1f3 | H1.3 linker histone, cluste... | NM_145713.4 | 80.5% | 90.9% | (many diffs) |
| 6 | mouse | 50708 | Hist1h1c | histone cluster 1, H1c | NM_015786.3 | 79.4% | 83.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 726
- ORF length:
- 657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccgagact gcgcctgccg cgcccgctgc tccggcccct gccgagaaga 121 ctcccgtgaa gaagaaggcc cgcaagtctg caggtgcggc caagcgcaaa gcgtctgggc 181 ccccggtgtc cgagctcatt actaaagctg ttgccgcctc caaggagcgc agcggcgtat 241 ctttggccgc tctcaagaaa gcgctggcag ccgctggcta tgacgtggag aagaacaaca 301 gccgcatcaa gctgggtctc aagagcctgg tgagcaaggg cacccTGGTG CAGACCAAGG 361 GCACCGGCGC GTCGGGTTCC TTCAAACTCA ACAAGAAGGC GGCCTCTGGG GAAGCCAAGC 421 CTAAGGCTAA AAAGGCAGGC GCGGCCAAGG CCAAGAAGCC AGCAGGAGCG GCGAAGAAGC 481 CCAAGAAGGC GACGGGGGCG GCCACCCCCA AGAAGAGCGC CAAGAAGACC CCAAAGAAGG 541 CGAAGAAGCC GGCTGCAGCT GCTGGAGCCA AAAAAGCGAA AAGCCCGAAA AAGGCGAAAG 601 CAGCCAAGCC AAAAAAGGCG CCCAAGAGCC CAGCGAAGGC CAAAGCAGTT AAACCCAAGG 661 CGGCTAAACC AAAGACCGCC AAGCCCAAGG CAGCCAAGCC AAAGAAGGCG GCAGCCAAGA 721 AAAAGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 781 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 841 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGTATACGA TAATTTCTCC AAAAAACGCG 901 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt