Construct: ORF TRCN0000467233
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017777.1_s317c1
- Derived from:
- ccsbBroadEn_11429
- DNA Barcode:
- TAACACCTGACTCACCTCTCTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRGAP3 (9901)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467233
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XM_017007580.2 | 46.3% | 45.6% | (many diffs) |
| 2 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XM_017007579.1 | 37.5% | 36.9% | (many diffs) |
| 3 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XM_017007576.2 | 33.6% | 33% | (many diffs) |
| 4 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XM_024453843.1 | 33.4% | 32.9% | (many diffs) |
| 5 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XM_024453844.1 | 33.4% | 32.9% | (many diffs) |
| 6 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | NM_001033117.3 | 30.6% | 30.1% | (many diffs) |
| 7 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XM_011534296.2 | 30.3% | 29.8% | (many diffs) |
| 8 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XM_017007575.1 | 30.1% | 29.6% | (many diffs) |
| 9 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | NM_014850.4 | 29.9% | 29.4% | (many diffs) |
| 10 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XM_011534295.2 | 29.8% | 29.3% | (many diffs) |
| 11 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XM_017007574.1 | 29.4% | 29% | (many diffs) |
| 12 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XR_001740391.1 | 29.3% | (many diffs) | |
| 13 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XM_011534301.3 | 29% | 28.5% | (many diffs) |
| 14 | human | 9901 | SRGAP3 | SLIT-ROBO Rho GTPase activa... | XM_024453842.1 | 27.9% | 27.4% | (many diffs) |
| 15 | mouse | 259302 | Srgap3 | SLIT-ROBO Rho GTPase activa... | XM_006506147.2 | 63.7% | 67% | (many diffs) |
| 16 | mouse | 259302 | Srgap3 | SLIT-ROBO Rho GTPase activa... | XM_006506146.1 | 35.4% | 37.1% | (many diffs) |
| 17 | mouse | 259302 | Srgap3 | SLIT-ROBO Rho GTPase activa... | XM_006506145.1 | 28.4% | 29.8% | (many diffs) |
| 18 | mouse | 259302 | Srgap3 | SLIT-ROBO Rho GTPase activa... | NM_080448.4 | 27.8% | 29.2% | (many diffs) |
| 19 | mouse | 259302 | Srgap3 | SLIT-ROBO Rho GTPase activa... | XM_006506144.1 | 27.7% | 29% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1071
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa caatgtcatc gtccgcctct cccagatcag tgaggatgtc atcagactct 121 tcaaaaagag caaggagatt ggcctgcaga tgcacgagga gctcctgaag gtgaccaatg 181 agctctacac agtcatgaaa acctaccaca tgtaccatgc agagagcatc agtgcggaaa 241 gcaagctgaa ggaggctgag aagcaggagg agaagcagtt caataagtca ggagacctca 301 gcatgaacct gctccggcac gaggaccggc cccagcgccg cagctctgtg aagaagattg 361 agaagatgaa ggagaagagg caggccaagt actctgagaa caagctgaaa tgcacaaagg 421 cccggaatga ctacttgctc aatctggcag ccaccaacgc agctataagc aaatactaca 481 tccatgatgt ctctgatctg atcgattgct gtgatttggg cttccatgcc agcctggccc 541 gcaccttccg gacctatctc tcagctgaat acaacctgga gacctctcgc cacgaagggc 601 tggatgtcat tgagaatgca gtggacaacc tggattcccg aagtgacaag cacacagtca 661 tggacatgtg caatcaagtc ttctgccctc cactcaagtt cgagttccag ccccacatgg 721 gggatgaggt ctgccaggtc agcgctcagc agcccgtcca gacagaactg ctcatgcgtt 781 atcaccagct gcagtccaga ctggCCACCC TCAAGATAGA GAATGAGGAG GTTAGGAAAA 841 CCCTGGATGC CACCATGCAG ACATTACAGG ACATGCTGAC TGTGGAGGAC TTTGATGTCT 901 CCGATGCCTT CCAACACAGT CGATCGACAG AGTCCGTCAA GTCGGCTGCC TCTGAGGCCT 961 ACATGAGCAA GATCAACATT GCCAAGAGGA GAGCCAACCA GCAGGAAACA GAAATGTTTT 1021 ATTTTACAAA CGGGCCTGAT GATGTTTTCC CCATTTGCCA TCCAAGACTC TGCCCAACTT 1081 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1141 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1201 CTTGTGGAAA GGACGATAAC ACCTGACTCA CCTCTCTGGT ACGCGTTAAG TCgacaatca 1261 acctctggat tacaaaattt gtgaaagatt