Construct: ORF TRCN0000467243
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001426.1_s317c1
- Derived from:
- ccsbBroadEn_07510
- DNA Barcode:
- AGGGGGGAGTATGCTCTATGCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF10 (9921)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467243
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9921 | RNF10 | ring finger protein 10 | NM_014868.5 | 99.9% | 99.8% | 996G>T |
| 2 | human | 9921 | RNF10 | ring finger protein 10 | NM_001330474.2 | 99.3% | 99.2% | 996G>T;1253_1267del |
| 3 | human | 9921 | RNF10 | ring finger protein 10 | XM_017020282.1 | 93.6% | 93.4% | (many diffs) |
| 4 | human | 9921 | RNF10 | ring finger protein 10 | XM_005254013.2 | 93% | 92.8% | (many diffs) |
| 5 | human | 9921 | RNF10 | ring finger protein 10 | XM_017020283.2 | 86.9% | 83.4% | (many diffs) |
| 6 | human | 9921 | RNF10 | ring finger protein 10 | XR_001748934.2 | 74.9% | (many diffs) | |
| 7 | human | 9921 | RNF10 | ring finger protein 10 | XR_001748933.2 | 63.4% | (many diffs) | |
| 8 | human | 9921 | RNF10 | ring finger protein 10 | XR_944849.3 | 63.1% | (many diffs) | |
| 9 | human | 9921 | RNF10 | ring finger protein 10 | XR_001748935.2 | 62.5% | (many diffs) | |
| 10 | human | 9921 | RNF10 | ring finger protein 10 | XR_001748938.2 | 61.9% | (many diffs) | |
| 11 | human | 9921 | RNF10 | ring finger protein 10 | XR_001748937.2 | 61.7% | (many diffs) | |
| 12 | human | 9921 | RNF10 | ring finger protein 10 | XR_001748936.2 | 61% | (many diffs) | |
| 13 | human | 9921 | RNF10 | ring finger protein 10 | XR_243026.4 | 60.8% | (many diffs) | |
| 14 | human | 9921 | RNF10 | ring finger protein 10 | XR_001748939.1 | 59.5% | (many diffs) | |
| 15 | mouse | 50849 | Rnf10 | ring finger protein 10 | NM_016698.2 | 90.6% | 91.8% | (many diffs) |
| 16 | mouse | 50849 | Rnf10 | ring finger protein 10 | NM_001302448.1 | 90.5% | 91.7% | (many diffs) |
| 17 | mouse | 50849 | Rnf10 | ring finger protein 10 | NM_001302449.1 | 90.5% | 91.7% | (many diffs) |
| 18 | mouse | 50849 | Rnf10 | ring finger protein 10 | XM_017320970.2 | 90.4% | 91.6% | (many diffs) |
| 19 | mouse | 50849 | Rnf10 | ring finger protein 10 | XM_030254658.1 | 76.9% | 78.4% | (many diffs) |
| 20 | mouse | 50849 | Rnf10 | ring finger protein 10 | XM_006530404.1 | 76.8% | 78.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2499
- ORF length:
- 2433
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gctgagctcc cccaacgccg ccgccaccgc ctccgacatg gacaagaaca 121 gcggctccaa cagctcctcc gcctcttcgg gcagcagcaa agggcaacag ccgccccgct 181 ccgcctcggc ggggccagcc ggcgagtcta aacccaagag cgatggaaag aactccagtg 241 gatccaagcg ttataatcgc aaacgtgaac tttcctaccc caaaaatgaa agttttaaca 301 accagtcccg tcgctccagt tcacagaaaa gcaagacttt taacaagatg cctcctcaaa 361 ggggcggcgg cagcagcaaa ctctttagct cttcttttaa tggtggaaga cgagatgagg 421 tagcagaggc tcaacgggca gagtttagcc ctgcccagtt ctctggtcct aagaagatca 481 acctgaacca cttgttgaat ttcacttttg aaccccgtgg ccagacgggt cactttgaag 541 gcagtggaca tggtagctgg ggaaagagga acaagtgggg acataagcct tttaacaagg 601 aactcttttt acaggccaac tgccaatttg tggtgtctga agaccaagac tacacagctc 661 attttgctga tcctgataca ttagttaact gggactttgt ggaacaagtg cgcatttgta 721 gccatgaagt gccatcttgc ccaatatgcc tctatccacc tactgcagcc aagataaccc 781 gttgtggaca catcttctgc tgggcatgca tcctgcacta tctttcactg agtgagaaga 841 cgtggagtaa atgtcccatc tgttacagtt ctgtgcataa gaaggatctc aagagtgttg 901 ttgccacaga gtcacatcag tatgttgttg gtgataccat tacgatgcag ctgatgaaga 961 gggagaaagg ggtgttggtg gctttgccca aatccaaatg gatgaatgta gaccatccca 1021 ttcatctagg agatgaacag cacagccagt actccaagtt tctgctggcc tctaaggagc 1081 aggtgctgca ccgggtagtt ctggaggaga aagtagcact agagcagcag ctggcagagg 1141 agaagcacac tcccgagtcc tgctttattg aggcagctat ccaggagctc aagactcggg 1201 aagaggctct gtcgggattg gccggaagca gaagggaggt cactggtgtt gtggctgctc 1261 tggaacaact ggtgctgatg gctcccttgg cgaaggagtc tgtttttcaa cccaggaagg 1321 gtgtgctgga gtatctgtct gccttcgatg aagaaaccac ggaagtttgt tctctggaca 1381 ctccttctag acctcttgct ctccctctgg tagaagagga ggaagcagtg tctgaaccag 1441 agcctgaggg gttgccagag gcctgtgatg acttggagtt agcagatgac aatcttaaag 1501 aggggaccat ttgcactgag tccagccagc aggaacccat caccaagtca ggcttcacac 1561 gcctcagcag ctctccttgt tactactttt accaagcgga agatggacag catatgttcc 1621 tgcaccctgt gaatgtgcgc tgcctcgtgc gggagtacgg cagcctggag aggagccccg 1681 agaagatctc agcaactgtg gtggagattg ctggctactc catgtctgag gatgttcgac 1741 agcgtcacag atatctctct cacttgccac tcacctgtga gttcagcatc tgtgaactgg 1801 ctttgcaacc tcctgtggtc tctaaggaaa ccctagagat gttctcagat gacattgaga 1861 agaggaaacg tcagcgccaa aagaaggctc gggaggaacg ccgccgagag cgcaggattg 1921 agatagagga gaacaagaaa cagggcaagt acccagaagt ccacattccc ctcgagaatc 1981 tacagcagtt tcctgccttc aattcttata cctgctcctc tgattctgct ttgggtccca 2041 ccagcaccga gggccatggg gccctctcca tttctcctct cagcagaagt ccaggttccc 2101 atgcagactt tctgctgacc cctctgtcac ccactgccag tcagggcagt ccctcattcT 2161 GCGTTGGGAG TCTGGAAGAA GACTCTCCCT TCCCTTCCTT TGCCCAGATG CTGAGGGTTG 2221 GAAAAGCAAA AGCAGATGTG TGGCCCAAAA CTGCTCCAAA GAAAGATGAG AACAGCTTAG 2281 TTCCTCCTGC CCCTGTGGAC AGCGACGGGG AGAGTGATAA TTCAGACCGT GTTCCTGTGC 2341 CCAGTTTTCA AAATTCCTTC AGCCAAGCTA TTGAAGCAGC CTTCATGAAA CTGGACACAC 2401 CAGCTACTTC AGATCCCCTC TCTGAAGAGA AAGGAGGAAA GAAAAGAAAA AAACAGAAAC 2461 AGAAGCTCCT GTTCAGCACC TCAGTCGTCC ACACCAAGTG CCCAACTTTC TTGTACAAAG 2521 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2581 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2641 ACGAAGGGGG GAGTATGCTC TATGCATAAC GCGTTAAGTC gacaatcaac ctctggatta 2701 caaaatttgt gaaagatt