Construct: ORF TRCN0000467250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017633.1_s317c1
- Derived from:
- ccsbBroadEn_04366
- DNA Barcode:
- ACATCTTCCGGCGTCTCCTCGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHCHD6 (84303)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467250
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | NM_032343.3 | 100% | 100% | |
| 2 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | NM_001320610.2 | 99.5% | 99.5% | 411_413delGCA |
| 3 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XM_017007321.1 | 85% | 82.2% | (many diffs) |
| 4 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XM_024453792.1 | 84.2% | 84.2% | 0_1ins111 |
| 5 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XM_011513234.1 | 83.8% | 83.8% | 0_1ins111;300_302delGCA |
| 6 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XM_017007320.1 | 82.1% | 75.9% | (many diffs) |
| 7 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XR_924200.1 | 80.8% | 1_72del;483_573del;865_866insGGC | |
| 8 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XM_017007322.2 | 73.1% | 71.6% | (many diffs) |
| 9 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XR_924193.2 | 63.6% | (many diffs) | |
| 10 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XR_924195.2 | 61.4% | (many diffs) | |
| 11 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XM_011513235.3 | 60.4% | 59.5% | (many diffs) |
| 12 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XR_924199.2 | 57% | 1_72del;639_889del;1029_1236del | |
| 13 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XR_924198.2 | 56.9% | (many diffs) | |
| 14 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XR_924192.2 | 55.6% | (many diffs) | |
| 15 | human | 84303 | CHCHD6 | coiled-coil-helix-coiled-co... | XR_924196.2 | 54.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 771
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gagcacggag agcagcgagg gccgcagggt gtccttcgga gtggacgagg 121 aggagcgggt ccgggtgctg cagggtgtcc ggctgtctga aaacgtggtg aaccgcatga 181 aggagcccag ctctccaccc cctgctccca catcttctac ctttggcctt caagatggca 241 acttgagagc ccctcacaaa gaatccacac tgcccaggtc ggggagcagt ggtggccagc 301 agcccTCAGG GATGAAGGAG GGTGTCAAGA GGTATGAACA GGAGCATGCT GCTATCCAGG 361 ATAAGCTCTT CCAGGTGGCA AAGAGGGAAA GAGAGGCTGC CACCAAGCAC TCCAAGGCAT 421 CCCTGCCCAC GGGCGAAGGC AGCATCAGCC ATGAGGAGCA GAAGTCAGTC CGGCTGGCCA 481 GGGAGCTGGA GAGCAGAGAG GCAGAGCTAA GACGCCGTGA CACCTTCTAC AAGGAGCAGC 541 TGGAGCGTAT TGAGAGGAAG AATGCTGAGA TGTATAAACT GTCTTCAGAG CAATTCCATG 601 AGGCAGCCTC AAAGATGGAG AGCACAATAA AGCCCCGCAG GGTGGAGCCC GTCTGCTCAG 661 GGTTGCAGGC CCAGATTCTC CACTGCTACC GAGATCGCCC GCATGAGGTG CTGCTGTGCT 721 CGGACCTGGT CAAGGCATAC CAGCGCTGCG TGAGCGCCGC CCACAAGGGC TGCCCAACTT 781 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 841 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 901 CTTGTGGAAA GGACGAACAT CTTCCGGCGT CTCCTCGCAG ACGCGTTAAG TCgacaatca 961 acctctggat tacaaaattt gtgaaagatt