Construct: ORF TRCN0000467287
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018920.2_s317c1
- Derived from:
- ccsbBroadEn_14988
- DNA Barcode:
- ACCAAATTTTTTACCGGGCTGTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP4K1 (11184)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467287
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11184 | MAP4K1 | mitogen-activated protein k... | NM_001042600.3 | 99.2% | 30.9% | (many diffs) |
| 2 | human | 11184 | MAP4K1 | mitogen-activated protein k... | NM_007181.6 | 96.7% | 30.4% | (many diffs) |
| 3 | human | 11184 | MAP4K1 | mitogen-activated protein k... | XM_011526404.1 | 94.6% | 29.5% | (many diffs) |
| 4 | human | 11184 | MAP4K1 | mitogen-activated protein k... | XM_011526403.1 | 92.3% | 29% | (many diffs) |
| 5 | human | 11184 | MAP4K1 | mitogen-activated protein k... | XM_017026231.1 | 89.7% | 25.5% | (many diffs) |
| 6 | mouse | 26411 | Map4k1 | mitogen-activated protein k... | NM_008279.2 | 83.3% | 28.8% | (many diffs) |
| 7 | mouse | 26411 | Map4k1 | mitogen-activated protein k... | XM_006539995.2 | 82.7% | 29.4% | (many diffs) |
| 8 | mouse | 26411 | Map4k1 | mitogen-activated protein k... | XM_006539996.2 | 80.2% | 29.9% | (many diffs) |
| 9 | mouse | 26411 | Map4k1 | mitogen-activated protein k... | XM_006539997.3 | 80% | 30% | (many diffs) |
| 10 | mouse | 26411 | Map4k1 | mitogen-activated protein k... | XM_017322280.1 | 78.8% | 23.9% | (many diffs) |
| 11 | mouse | 26411 | Map4k1 | mitogen-activated protein k... | XM_006539998.2 | 76.9% | 31.2% | (many diffs) |
| 12 | mouse | 26411 | Map4k1 | mitogen-activated protein k... | XM_006539999.1 | 75.6% | 24.8% | (many diffs) |
| 13 | mouse | 26411 | Map4k1 | mitogen-activated protein k... | XM_006540000.2 | 73.6% | 32.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 837
- ORF length:
- 768
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacgtcgtg gaccctgaca ttttcaatag agacccccgg gaccactatg 121 acctgctaca gcggctgggt ggcggcacgt atggggaagt ctttaaggct cgagacaagg 181 tgtcagggga cctggtggca ctgaagatgg tgaagatgga gcctgatgat gatgtctcca 241 cccttcagaa ggaaatcctc atattgaaaa cttgccggca cgccaacatc gtggcctacc 301 atgggagtta tctctggttg cagaaactct ggatctgcat ggaattctgt ggggctggtt 361 ctctccagga catctaccaa gtgacaggct ccctgtcaga gctccagatt agctatgtct 421 gccgggaagt gctccaggga ctggcctatt tgcactcaca gaagaagata cacagggaca 481 tcaagggagc taacatcctc atcaatgatg ctggggaggt cagattggct gactttggca 541 tctcggccca gattggggct acactggcca gacgcctctc tttcattggg acaccctact 601 ggatggctcc ggaagtggca gctgtggccc tgaagggagg atacaatgag ctgtgtgaca 661 tctggtccct gggcatcacg gccatcgaac tggccgagct acagccaccg ctctttgatg 721 tgcaccctct cagagttctc ttcctcatga ccaagagtgg ctaccagcct ccccgactga 781 aggaaaaagg caaatggtcg gctgccttcc acaacttcat caaagtcact tctgactaag 841 agtcccaaga aacgacccag cgccaccaag atgctcagtc atcaactggt atcccagcct 901 gggctgaatc gaggcctgat cctggatctt cttgacaaac tgaagaatcc cgggaaagga 961 ccctccattg gggacattga ggatgaggag cccgagctac cccctgctat ccctcggcgg 1021 atcagatcca cccaccgctc cagctctctg gggatcccag atgcagactg ctgtcggcgg 1081 cacatggagt tcaggaagct ccgaggaatg gagaccagac ccccagccaa caccgctcgc 1141 ctacagcctc ctcgagacct caggagcagc agccccagga agcaactgtc agagtcgtct 1201 gacgatgact atgacgacgt ggacatcccc acccctgcag aggacacacc tcctccactt 1261 ccccccaagc ccaagttccg ttctccatca gacgagggtc ctgggagcat gggggatgat 1321 gggcagctga gcccgggggt gctggtccgg tgtgccagtg ggcccccacc aaacagcccc 1381 cgtcctgggc ctcccccatc caccagcagc ccccacctca ccgcccattc agaaccctca 1441 ctctggaacc caccctcccg ggagcttgac aagcccccac ttctgccccc caagaaggaa 1501 aagatgaaga gaaagggatg tgcccttctc gtaaagttgt tcaatggctg ccccctccgg 1561 atccacagca cggccgcctg gacacatccc tccaccaagg accagcacct gctcctgggg 1621 gcagaggaag gcatcttcat cctgaaccgg aatgaccagg aggccacgct ggaaatgctc 1681 tttcctagcc ggactacgtg ggtgtactcc atcaacaacg ttctcatgtc tctctcagga 1741 aagacccccc ccttttattc tcatagcatc ccttgccctg ctggaacggg aaagagacca 1801 gaagcaggaa acccccatcg gttcacatta gccccccacc gcctactgcn aanggagaaa 1861 catggtttcc accaangatc caggacacca aagggctgcc gggcgtgctg tgtggcggag 1921 ggtgcgagct ctgggggccc gttcctgtgc ggtgcattgg agacgtccgt tgtcctgctt 1981 cagtggtacc agcccatgaa caaattcctg cttgtccggc aggtgctgtt cccactgccg 2041 acgcctctgt ccgtgttcgc gctgctgacc gggccaggct ctgagctgcc cgctgtgtgc 2101 atcggcgtga gccccgggcg gccggggaag tcggtgctct tccacacggt gcgctttggc 2161 gcgctctctt gctggctggg cgagatgagc accgagcaca ggggacCCGT GCAGGTGACC 2221 CAGGTAGAGG AAGATATGGT GATGGTGTTG ATGGATGGCT CTGTGAAGCT GGTGACCCCG 2281 GAGGGGTCCC CAGTCCGGGG ACTTCGCACA CCTGAGATCC CCATGACCGA AGCGGTGGAG 2341 GCCGTGGCTA TGGTTGGAGG TCAGCTTCAG GCCTTCTGGA AGCATGGAGT GCAGGTGTGG 2401 GCTCTAGGCT CGGATCAGCT GCTACAGGAG CTGAGAGACC CTACCCTCAC TTTCCGTCTG 2461 CTTGGCTCCC CCAGGCCTGT AGTGGTGGAG ACACGCCCAG TGGATGATCC TACTGCTCCC 2521 AGCAACCTCT ACATCCAGGA ATTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG 2581 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT 2641 GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACC AAATTTTTTA 2701 CCGGGCTGTT TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 2761 t