Construct: ORF TRCN0000467327
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010350.1_s317c1
- Derived from:
- ccsbBroadEn_04604
- DNA Barcode:
- ATAAGTGAACCAGAGCAAGCGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXO44 (93611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467327
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93611 | FBXO44 | F-box protein 44 | NM_001304790.1 | 100% | 100% | |
| 2 | human | 93611 | FBXO44 | F-box protein 44 | NM_183412.3 | 100% | 100% | |
| 3 | human | 93611 | FBXO44 | F-box protein 44 | NM_183413.2 | 100% | 100% | |
| 4 | human | 93611 | FBXO44 | F-box protein 44 | XM_017002844.2 | 100% | 100% | |
| 5 | human | 93611 | FBXO44 | F-box protein 44 | XM_024450967.1 | 100% | 100% | |
| 6 | human | 93611 | FBXO44 | F-box protein 44 | XM_024450974.1 | 88.1% | 88.1% | 1_90del |
| 7 | human | 93611 | FBXO44 | F-box protein 44 | NM_001330355.1 | 87.5% | 87.5% | 363_458del |
| 8 | human | 93611 | FBXO44 | F-box protein 44 | XM_005263536.4 | 87.5% | 87.5% | 363_458del |
| 9 | human | 93611 | FBXO44 | F-box protein 44 | XM_005263537.1 | 87.5% | 87.5% | 363_458del |
| 10 | human | 93611 | FBXO44 | F-box protein 44 | XM_006711043.3 | 87.5% | 87.5% | 363_458del |
| 11 | human | 93611 | FBXO44 | F-box protein 44 | XM_011542435.1 | 87.5% | 87.5% | 363_458del |
| 12 | human | 93611 | FBXO44 | F-box protein 44 | XM_017002842.2 | 87.5% | 87.5% | 363_458del |
| 13 | human | 93611 | FBXO44 | F-box protein 44 | NM_001014765.1 | 80.3% | 47% | 363_487del;765_766ins32 |
| 14 | human | 93611 | FBXO44 | F-box protein 44 | NM_001304791.2 | 80.3% | 47% | 363_487del;765_766ins32 |
| 15 | human | 93611 | FBXO44 | F-box protein 44 | NM_033182.7 | 80.3% | 47% | 363_487del;765_766ins32 |
| 16 | human | 93611 | FBXO44 | F-box protein 44 | XM_006711045.3 | 80.3% | 47% | 363_487del;765_766ins32 |
| 17 | human | 93611 | FBXO44 | F-box protein 44 | XM_017002843.2 | 80.3% | 47% | 363_487del;765_766ins32 |
| 18 | human | 93611 | FBXO44 | F-box protein 44 | NR_130909.1 | 23% | (many diffs) | |
| 19 | mouse | 230903 | Fbxo44 | F-box protein 44 | NM_001161852.2 | 88.5% | 87.5% | (many diffs) |
| 20 | mouse | 230903 | Fbxo44 | F-box protein 44 | NM_001161851.2 | 71.7% | 44.7% | (many diffs) |
| 21 | mouse | 230903 | Fbxo44 | F-box protein 44 | NM_173401.5 | 71.7% | 44.7% | (many diffs) |
| 22 | mouse | 230903 | Fbxo44 | F-box protein 44 | XM_017320176.1 | 71.7% | 44.7% | (many diffs) |
| 23 | mouse | 230903 | Fbxo44 | F-box protein 44 | XM_017320177.1 | 71.7% | 44.7% | (many diffs) |
| 24 | mouse | 230903 | Fbxo44 | F-box protein 44 | XM_017320178.1 | 71.7% | 44.7% | (many diffs) |
| 25 | mouse | 230903 | Fbxo44 | F-box protein 44 | XM_017320180.1 | 57.1% | 50% | (many diffs) |
| 26 | mouse | 230903 | Fbxo44 | F-box protein 44 | XM_011250236.2 | 55.8% | 52.3% | (many diffs) |
| 27 | mouse | 230903 | Fbxo44 | F-box protein 44 | XM_017320179.1 | 45.4% | 17.5% | (many diffs) |
| 28 | mouse | 230903 | Fbxo44 | F-box protein 44 | XR_881428.2 | 42% | (many diffs) | |
| 29 | mouse | 230903 | Fbxo44 | F-box protein 44 | NR_104374.1 | 35.9% | (many diffs) | |
| 30 | mouse | 230903 | Fbxo44 | F-box protein 44 | NR_104375.1 | 35.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 738
- ORF length:
- 672
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtggggaac atcaacgagc tgcccgagaa catcctgctg gagctgttca 121 cgcacgtgcc cgcccgccag ctgctgctga actgccgcct ggtctgcagc ctctggcggg 181 acctcatcga cctcgtgacc ctctggaaac gcaagtgcct gcgagagggc ttcatcactg 241 aggactggga ccagcccgtg gccgactgga agatcttcta cttcttacgg agcctgcaca 301 ggaacctcct gcacaacccg tgcgctgaag aggggttcga gttctGGAGC CTGGATGTGA 361 ATGGAGGCGA TGAGTGGAAG GTGGAGGATC TCTCTCGAGA CCAGAGGAAG GAATTCCCCA 421 ATGACCAGGT TCGCAGCCAG GCCAGATTGC GGGTCCAAGT ACCAGCTGTG CGTTCAGCTC 481 CTGTCGTCCG CGCACGCGCC TCTGGGGACC TTCCAGCCAG ACCCGGCGAC CATCCAGCAG 541 AAGAGCGATG CCAAGTGGAG GGAGGTCTCC CACACATTCT CCAACTACCC GCCCGGCGTC 601 CGCTACATCT GGTTTCAGCA CGGCGGCGTG GACACTCATT ACTGGGCCGG CTGGTACGGC 661 CCGAGGGTCA CCAACAGCAG CATCACCATC GGGCCCCCGC TGCCCTGACA CCCCCTGAGC 721 CCCCATCTGC TGAACCCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 781 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 841 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATAAGTG AACCAGAGCA 901 AGCGTTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt