Construct: ORF TRCN0000467460
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009398.1_s317c1
- Derived from:
- ccsbBroadEn_03561
- DNA Barcode:
- CCCAGTGGGGTCCGGGTCAACTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PACC1 (55248)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467460
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55248 | PACC1 | proton activated chloride c... | NM_018252.3 | 100% | 100% | |
| 2 | human | 55248 | PACC1 | proton activated chloride c... | NM_001198862.1 | 85.1% | 85.1% | 35_217del |
| 3 | human | 55248 | PACC1 | proton activated chloride c... | XM_017001635.1 | 80% | 80% | 129_130ins210 |
| 4 | human | 55248 | PACC1 | proton activated chloride c... | XM_011509717.3 | 79.7% | 77% | (many diffs) |
| 5 | human | 55248 | PACC1 | proton activated chloride c... | XM_005273177.3 | 78% | 78% | 0_1ins231 |
| 6 | human | 55248 | PACC1 | proton activated chloride c... | XM_017001636.1 | 78% | 78% | 0_1ins231 |
| 7 | human | 55248 | PACC1 | proton activated chloride c... | XM_017001637.2 | 71.1% | 59.8% | 636_637ins145;747_748ins158 |
| 8 | human | 55248 | PACC1 | proton activated chloride c... | XM_017001634.1 | 63.6% | 61.2% | (many diffs) |
| 9 | human | 55248 | PACC1 | proton activated chloride c... | XM_011509718.3 | 56.5% | 45.7% | (many diffs) |
| 10 | mouse | 66950 | Pacc1 | proton activated chloride c... | NM_025864.4 | 86.3% | 90.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ccggcaggag cgctccacat cctaccagga gctgagtgag gagttggtcc 121 aggtggttga gaactcagag ctggcagacg agcaggacaa ggagacggtc agagtccaag 181 gtccgggtat cttaccaggc ctggacagcg agtccgcctc cagcagcatc cgcttcagca 241 aggcctgcct gaagaacgtc ttctcggtcc tactcatctt catctacctg ctgctcatgg 301 ctgtggccgt cttcctggtc taccggacca tcacagactt tcgtgagaaa ctcaagcacc 361 ctgtcatgtc tgtgtcttac aaggaagtgg atcgctatga tgccccaggt attgccttgt 421 accccggtca ggcccagttg ctcagctgta agcaccatta cgaggtcatt cctcctctga 481 caagccctgg ccagccgggt gacatgaatt gcaccaccca gaggatcaac tacacggacc 541 ccttctccaa tcagactgtg aaatctgccc tgattgtcca ggggccccgg gaagtgaaaa 601 agcgggagct ggtcttcctc cagttccgcc tgaacaagag tagtgaggac ttcagcgcca 661 ttgattacct cctcttctcT TCTTTCCAGG AGTTCCTGCA AAGCCCAAAC AGGGTAGGCT 721 TCATGCAGGC CTGTGAGAGT GCCTATTCCA GCTGGAAGTT CTCTGGGGGC TTCCGCACCT 781 GGGTCAAGAT GTCACTGGTA AAGACCAAGG AGGAGGATGG GCGGGAAGCA GTGGAGTTCC 841 GGCAGGAGAC AAGTGTGGTT AACTACATTG ACCAGAGGCC AGCTGCCAAA AAAAGTGCTC 901 AATTGTTTTT TGTGGTCTTT GAATGGAAAG ATCCTTTCAT CCAGAAAGTC CAAGATATAG 961 TCACTGCCAA TCCTTGGAAC ACAATTGCTC TTCTCTGTGG CGCCTTCTTG GCATTATTTA 1021 AAGCAGCAGA GTTTGCCAAA CTGAGTATAA AATGGATGAT CAAAATTAGA AAGAGATACC 1081 TTAAAAGAAG AGGTCAGGCA ACGAGCCACA TAAGCTGCCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 ACCCAGTGGG GTCCGGGTCA ACTCGACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt