Construct: ORF TRCN0000467473
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014301.1_s317c1
- Derived from:
- ccsbBroadEn_12121
- DNA Barcode:
- TGCCGCCCGGAACTAGAAACGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PARP16 (54956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467473
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | NM_001316943.2 | 99.7% | 99.6% | 561G>A;838T>C |
| 2 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | NM_017851.6 | 99.4% | 99.3% | 561G>A;832_834delAGC;841T>C |
| 3 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XM_006720590.3 | 95.2% | 93.5% | (many diffs) |
| 4 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XM_006720589.3 | 94.9% | 93.2% | (many diffs) |
| 5 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XM_017022386.2 | 93.8% | 93.7% | 254_255ins57;504G>A;781T>C |
| 6 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XM_006720592.3 | 93.6% | 93.4% | (many diffs) |
| 7 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XM_017022387.2 | 85.5% | 85.4% | 173_174ins138;423G>A;700T>C |
| 8 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XM_011521742.3 | 85.2% | 85.1% | (many diffs) |
| 9 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XM_024449969.1 | 80.8% | 79.8% | (many diffs) |
| 10 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XM_017022389.2 | 78.3% | 78.2% | 310_311ins207;354G>A;631T>C |
| 11 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XM_017022388.2 | 78.1% | 78% | (many diffs) |
| 12 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XM_024449970.1 | 73.9% | 72.9% | (many diffs) |
| 13 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | NM_001316944.2 | 64% | 63.9% | 173_174ins345;216G>A;493T>C |
| 14 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XR_001751342.2 | 61.4% | (many diffs) | |
| 15 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XR_001751341.2 | 57% | (many diffs) | |
| 16 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XR_429460.3 | 55.7% | (many diffs) | |
| 17 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XR_429459.3 | 55.6% | (many diffs) | |
| 18 | human | 54956 | PARP16 | poly(ADP-ribose) polymerase... | XR_001751343.2 | 52.1% | (many diffs) | |
| 19 | mouse | 214424 | Parp16 | poly (ADP-ribose) polymeras... | NM_177460.4 | 86.9% | 90% | (many diffs) |
| 20 | mouse | 214424 | Parp16 | poly (ADP-ribose) polymeras... | XM_011242702.1 | 68.1% | 69.8% | (many diffs) |
| 21 | mouse | 214424 | Parp16 | poly (ADP-ribose) polymeras... | XM_006511005.1 | 49.8% | 49.3% | (many diffs) |
| 22 | mouse | 214424 | Parp16 | poly (ADP-ribose) polymeras... | XR_870755.2 | 29% | (many diffs) | |
| 23 | mouse | 214424 | Parp16 | poly (ADP-ribose) polymeras... | XR_001778840.1 | 25% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1032
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gccctcaggc tgggcggccg ccagggaggc ggcgggccgc gacatgctgg 121 ccgccgacct ccggtgcagc ctcttcgcct cggccctgca gagctacaag cgcgactcgg 181 tgctgcggcc cttccccgcg tcctacgccc gcggcgactg taaggacttt gaagccctgc 241 ttgcagatgc cagcaagtta cctaacctga aagaacttct ccagtcctcc ggagacaacc 301 acaaacgggc ctgggacctg gtgagctgga ttttatcctc aaaggtcctg acaatccaca 361 gtgcagggaa ggcagagttt gaaaagatcc aaaagctgac tggggctcct cacacgcctg 421 ttcctgcacc ggacttcctg tttgaaattg agtactttga cccagccaac gccaaatttt 481 atgagaccaa aggagaacga gacctaatct atgcatttca tggtagccgc ctagaaaact 541 tccattccat tatccacaat ggcctgcact gccatctgaa caagacatcc ttgttcggag 601 aggggaccta cctcaccagt gacttaagcc tggccctcat atacagcccc catggccatg 661 ggtggcagca cagcctcctc ggccccatcc ttagctgtgt ggccgtgtgt gaggtcattg 721 accatccgga cgtcaagtgc caaaccaaga agaaggattc caaggagata gatcGCAGAC 781 GAGCGAGAAT CAAACATAGT GAAGGGGGAG ACATCCCTCC CAAGTACTTC GTGGTCACCA 841 ATAACCAGCT GCTGCGAGTG AAGTACCTCC TGGTGTATTC ACAGAAGCCA CCCAAGAGGG 901 CTCCGAGCCA GCTCTCCTGG TTTTCCAGCC ATTGGTTTAC CGTCATGATA TCCCTGTATC 961 TGCTGCTGCT GCTCATAGTG AGTGTCATCA ACTCCTCTGC TTTCCAACAC TTTTGGAATC 1021 GTGCGAAAAG ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1141 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGC CGCCCGGAAC TAGAAACGCG 1201 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t