Construct: ORF TRCN0000467485
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016166.1_s317c1
- Derived from:
- ccsbBroadEn_01908
- DNA Barcode:
- CTCAATTAACCTTCGGAGAAGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H3C10 (8357)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467485
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8357 | H3C10 | H3 clustered histone 10 | NM_003536.2 | 100% | 100% | |
| 2 | human | 8351 | H3C4 | H3 clustered histone 4 | NM_003530.4 | 87.7% | 100% | (many diffs) |
| 3 | human | 8354 | H3C11 | H3 clustered histone 11 | NM_003533.2 | 87.5% | 100% | (many diffs) |
| 4 | human | 8968 | H3C7 | H3 clustered histone 7 | NM_021018.2 | 87.2% | 100% | (many diffs) |
| 5 | human | 333932 | H3C15 | H3 clustered histone 15 | NM_001005464.2 | 86.7% | 99.2% | (many diffs) |
| 6 | human | 126961 | H3C14 | H3 clustered histone 14 | NM_021059.2 | 86.7% | 99.2% | (many diffs) |
| 7 | human | 8355 | H3C8 | H3 clustered histone 8 | NM_003534.2 | 86.5% | 100% | (many diffs) |
| 8 | human | 8353 | H3C6 | H3 clustered histone 6 | NM_003532.2 | 86.2% | 100% | (many diffs) |
| 9 | human | 8290 | H3-4 | H3.4 histone | NM_003493.2 | 86.2% | 97% | (many diffs) |
| 10 | human | 653604 | H3C13 | H3 clustered histone 13 | NM_001123375.2 | 86% | 99.2% | (many diffs) |
| 11 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001355409.3 | 85% | 97% | (many diffs) |
| 12 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001372105.1 | 85% | 97% | (many diffs) |
| 13 | human | 8350 | H3C1 | H3 clustered histone 1 | NM_003529.2 | 85% | 100% | (many diffs) |
| 14 | human | 8356 | H3C12 | H3 clustered histone 12 | NM_003535.2 | 85% | 100% | (many diffs) |
| 15 | human | 8352 | H3C3 | H3 clustered histone 3 | NM_003531.2 | 84.8% | 100% | (many diffs) |
| 16 | human | 8358 | H3C2 | H3 clustered histone 2 | NM_003537.3 | 84.3% | 100% | (many diffs) |
| 17 | human | 3021 | H3-3B | H3.3 histone B | NM_005324.5 | 79.1% | 96.3% | (many diffs) |
| 18 | human | 440093 | H3-5 | H3.5 histone | NM_001013699.3 | 77.4% | 93.3% | (many diffs) |
| 19 | human | 3020 | H3-3A | H3.3 histone A | NM_002107.6 | 76.2% | 96.3% | (many diffs) |
| 20 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160943.1 | 32.6% | (many diffs) | |
| 21 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160941.1 | 17.8% | (many diffs) | |
| 22 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160940.1 | 15.9% | (many diffs) | |
| 23 | mouse | 319151 | Hist1h3e | histone cluster 1, H3e | NM_178205.2 | 88.7% | 99.2% | (many diffs) |
| 24 | mouse | 97908 | Hist1h3g | histone cluster 1, H3g | NM_145073.2 | 88.4% | 100% | (many diffs) |
| 25 | mouse | 319152 | Hist1h3h | histone cluster 1, H3h | NM_178206.2 | 88.4% | 100% | (many diffs) |
| 26 | mouse | 319153 | Hist1h3i | histone cluster 1, H3i | NM_178207.2 | 88.2% | 100% | (many diffs) |
| 27 | mouse | 260423 | Hist1h3f | histone cluster 1, H3f | NM_013548.4 | 88.2% | 99.2% | (many diffs) |
| 28 | mouse | 319148 | Hist1h3c | histone cluster 1, H3c | NM_175653.2 | 88.2% | 99.2% | (many diffs) |
| 29 | mouse | 319150 | Hist1h3b | histone cluster 1, H3b | NM_178203.2 | 88.2% | 99.2% | (many diffs) |
| 30 | mouse | 319149 | Hist1h3d | histone cluster 1, H3d | NM_178204.2 | 87.7% | 99.2% | (many diffs) |
| 31 | mouse | 360198 | Hist1h3a | histone cluster 1, H3a | NM_013550.5 | 87.5% | 100% | (many diffs) |
| 32 | mouse | 97114 | Hist2h3c2 | histone cluster 2, H3c2 | NM_054045.4 | 87.5% | 99.2% | (many diffs) |
| 33 | mouse | 15077 | Hist2h3c1 | histone cluster 2, H3c1 | NM_178216.3 | 87.5% | 99.2% | (many diffs) |
| 34 | mouse | 319154 | Hist2h3b | histone cluster 2, H3b | NM_178215.2 | 87.2% | 99.2% | (many diffs) |
| 35 | mouse | 382523 | Gm12260 | predicted gene 12260 | NM_001318003.2 | 85.5% | 97.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 474
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcgtacgaag cagactgctc gcaagtccac cGGCGGCAAG GCTCCGCGCA 121 AGCAGCTGGC CACCAAGGCG GCTCGGAAGA GCGCTCCGGC CACCGGCGGT GTCAAGAAGC 181 CCCATCGCTA TCGGCCTGGT ACAGTGGCTC TCCGCGAGAT TCGCCGCTAC CAGAAGTCCA 241 CCGAGCTGCT GATCAGAAAG CTGCCTTTTC AGCGTCTGGT GCGTGAGATC GCGCAGGACT 301 TCAAGACCGA CTTGCGCTTC CAGAGCTCCG CGGTGATGGC GCTGCAAGAG GCATGCGAGG 361 CCTACCTGGT GGGGCTCTTT GAGGACACCA ACCTGTGCGC CATCCACGCC AAGCGGGTGA 421 CTATCATGCC CAAGGACATC CAGCTCGCAC GTCGTATCCG CGGCGAGAGG GCTTGCCCAA 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAC TCAATTAACC TTCGGAGAAG TTCACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att