Construct: ORF TRCN0000467506
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015751.1_s317c1
- Derived from:
- ccsbBroadEn_08291
- DNA Barcode:
- CTTTGTACGCTATGCCTAGTGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HPCAL4 (51440)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467506
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51440 | HPCAL4 | hippocalcin like 4 | NM_001282396.1 | 99.6% | 98.9% | 11C>N;535C>G |
| 2 | human | 51440 | HPCAL4 | hippocalcin like 4 | NM_016257.4 | 99.6% | 98.9% | 11C>N;535C>G |
| 3 | human | 51440 | HPCAL4 | hippocalcin like 4 | XM_017001447.1 | 99.6% | 98.9% | 11C>N;535C>G |
| 4 | human | 7447 | VSNL1 | visinin like 1 | NM_001366803.1 | 80.6% | 89% | (many diffs) |
| 5 | human | 7447 | VSNL1 | visinin like 1 | NM_001366806.1 | 80.6% | 89% | (many diffs) |
| 6 | human | 7447 | VSNL1 | visinin like 1 | NM_003385.5 | 80.6% | 89% | (many diffs) |
| 7 | human | 51440 | HPCAL4 | hippocalcin like 4 | NM_001282397.1 | 61.9% | 61.2% | 11C>N;160_161ins216;319C>G |
| 8 | mouse | 170638 | Hpcal4 | hippocalcin-like 4 | NM_174998.4 | 93.1% | 98.9% | (many diffs) |
| 9 | mouse | 26950 | Vsnl1 | visinin-like 1 | NM_012038.4 | 82.3% | 89% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 639
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaagancaac agcaagctgg cccccgaggt gctggaggac cttgttcaga 121 acactgagtt cagcgagcag gagctgaagc agtggtacaa gggcttcctg aaggactgcc 181 ccagcggcat cctcaacctg gaggagtttc agcagctcta catcaagttc ttcccctacg 241 gcgacgcctc caagttcgcg cagcacgctt tccgcaccTT CGACAAGAAC GGCGACGGCA 301 CCATCGACTT CCGGGAGTTC ATCTGCGCCC TGTCGGTCAC CTCCCGCGGC AGCTTCGAGC 361 AGAAGCTCAA CTGGGCCTTT GAGATGTACG ACCTGGACGG CGACGGGCGC ATCACGCGCC 421 TGGAGATGCT GGAGATCATC GAGGCAATCT ACAAGATGGT GGGCACCGTG ATCATGATGC 481 GCATGAACCA GGACGGGCTC ACGCCCCAGC AGCGTGTGGA CAAGATCTTC AAGAAGATGG 541 ACCAGGATAA GGACGACCAG ATTACATTGG AGGAGTTCAA GGAGGCAGCC AAGAGTGACG 601 CATCCATTGT GTTGCTGCTG CAGTGTGACA TGCAGAAGTA CCCAACTTTC TTGTACAAAG 661 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 721 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 781 ACGACTTTGT ACGCTATGCC TAGTGATCAC GCGTTAAGTC gacaatcaac ctctggatta 841 caaaatttgt gaaagatt