Construct: ORF TRCN0000467508
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005233.1_s317c1
- Derived from:
- ccsbBroadEn_09383
- DNA Barcode:
- ACTTGTTACGTTATGCCGCGTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFAP36 (112942)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467508
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112942 | CFAP36 | cilia and flagella associat... | NM_080667.6 | 99.8% | 99.4% | 728A>G;736A>T |
| 2 | human | 112942 | CFAP36 | cilia and flagella associat... | NM_001282761.1 | 93% | 92.6% | 115_189del;803A>G;811A>T |
| 3 | human | 112942 | CFAP36 | cilia and flagella associat... | XM_017003254.1 | 64.7% | 60.8% | (many diffs) |
| 4 | human | 112942 | CFAP36 | cilia and flagella associat... | XM_017003255.1 | 64.7% | 60.8% | (many diffs) |
| 5 | human | 112942 | CFAP36 | cilia and flagella associat... | XM_011532499.1 | 55% | 54.6% | 0_1ins459;269A>G;277A>T |
| 6 | mouse | 216618 | Cfap36 | cilia and flagella associat... | NM_025740.3 | 88.4% | 90.6% | (many diffs) |
| 7 | mouse | 216618 | Cfap36 | cilia and flagella associat... | XM_017314441.1 | 47.9% | 47.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1092
- ORF length:
- 1026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcggaagaa gaagacgagg tggagtgggt agtggagagc atcgcggggt 121 tcctgcgagg cccagactgg tccatcccca tcttggactt tgtggaacag aaatgtgaag 181 tttttgatga tgaagaagaa agcaaattga cctatacaga gattcatcag gaatacaaag 241 aactagttga aaagctgtta gaaggttacc tcaaagaaat tggaattaat gaagatcaat 301 ttcaagaagc atgcacttct cctcttgcaa agacccatac atcacaggcc attttgcaac 361 ctgtgttggc agcagaagat tttactatct ttaaagcaat gatggtccag aaaaacattg 421 aaatgcagct gcaagccatt cgaataattc aagagagaaa tggtgtatta cctgactgct 481 taaccgatgg ctctgatgtg gtcagtgacc ttgaacacga agagatgaaa atcctgaggg 541 aagttcttag aaaatcaaaa gaggaatatg accaggaaga agaaaggaag aggaaaaaac 601 agttatcaga ggctaaaaca gaagagccca cagtgcattc cagtgaagct gcaataatga 661 ataattccca aggggatggt gaacattttg cacacccacc cTCAGAAGTT AAAATGCATT 721 TTGCTAATCA GTCAATAGAA CCTTTGGGAA GAAAAGTGGA AAGGTCTGAA ACTTCCTCCC 781 TCCCACAAAA AGGCCTGAAG TTTCCTGGCT TAGAGCATGC GAGCATTGAA GGACCAATAG 841 CAAACTTATC AGTACTTGGA ACAGAAGAAC TTCGGCAACG AGAACACTAT CTCAAGCAGA 901 AGAGAGATAA GTTGATGTCC ATGAGAAAGG ATATGAGGAC TAAACAGATA CAAAATATGG 961 AGCAGAAAGG AAAACCCACT GGGGAGGTAG AGGAAATGAC AGAGAAACCA GAAATGACAG 1021 CAGAGGAGAA GCAAACATTA CTAAAGAGGA GATTGCTTGC AGAGAAACTC AAAGAAGAAG 1081 TTATTAATAA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1141 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1201 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACT TGTTACGTTA TGCCGCGTAA 1261 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t