Construct: ORF TRCN0000467520

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001937.1_s317c1
Derived from:
ccsbBroadEn_01382
DNA Barcode:
CATTGTCTACCGGTTTAACTCAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RBM4 (5936)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467520
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5936 RBM4 RNA binding motif protein 4 NM_002896.3 100% 100%
2 human 83759 RBM4B RNA binding motif protein 4B NM_031492.4 85.7% 89.3% (many diffs)
3 human 83759 RBM4B RNA binding motif protein 4B XM_011545297.3 85.7% 89.3% (many diffs)
4 human 83759 RBM4B RNA binding motif protein 4B XM_017018402.2 85.7% 89.3% (many diffs)
5 human 100526737 RBM14-RBM4 RBM14-RBM4 readthrough NM_001198845.1 77.2% 74.5% (many diffs)
6 human 83759 RBM4B RNA binding motif protein 4B XR_247214.3 46.2% (many diffs)
7 human 83759 RBM4B RNA binding motif protein 4B XR_247213.3 44.4% (many diffs)
8 human 5936 RBM4 RNA binding motif protein 4 NM_001198844.2 44.3% 38.6% (many diffs)
9 human 5936 RBM4 RNA binding motif protein 4 NM_001198843.1 38.8% 38.4% (many diffs)
10 human 83759 RBM4B RNA binding motif protein 4B NM_001286135.2 37.5% 37.3% (many diffs)
11 mouse 19653 Rbm4 RNA binding motif protein 4 NM_001290122.1 92.8% 95.8% (many diffs)
12 mouse 19653 Rbm4 RNA binding motif protein 4 NM_001290123.1 92.8% 95.8% (many diffs)
13 mouse 19653 Rbm4 RNA binding motif protein 4 NM_009032.3 92.8% 95.8% (many diffs)
14 mouse 66704 Rbm4b RNA binding motif protein 4B NM_025717.3 82.6% 87.6% (many diffs)
15 mouse 66704 Rbm4b RNA binding motif protein 4B XM_006531823.3 82.6% 87.6% (many diffs)
16 mouse 66704 Rbm4b RNA binding motif protein 4B XM_006531824.3 82.6% 87.6% (many diffs)
17 mouse 66704 Rbm4b RNA binding motif protein 4B XM_006531825.3 82.6% 87.6% (many diffs)
18 mouse 66704 Rbm4b RNA binding motif protein 4B XM_006531826.2 82.6% 87.6% (many diffs)
19 mouse 102902673 Gm21992 predicted gene 21992 NM_001290128.1 72.9% 71.1% (many diffs)
20 mouse 102902673 Gm21992 predicted gene 21992 NM_001290127.1 70.4% 72.7% (many diffs)
21 mouse 66704 Rbm4b RNA binding motif protein 4B XM_006531827.3 38.2% 37.9% (many diffs)
22 mouse 19653 Rbm4 RNA binding motif protein 4 NM_001290124.1 35.9% 37.9% (many diffs)
23 mouse 19653 Rbm4 RNA binding motif protein 4 NM_001290125.1 35.9% 37.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1158
ORF length:
1092
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gaagctgttc atcggaaacc tgccccggga ggctacagag caggagattc
121gctcactctt cgagcagtat gggaaggtgc tggaatgtga catcattaag aattacggct
181ttgtgcacat agaagacaag acggcagctg aggatgccat acgcaacctg caccattaca
241agcttcatgg ggtgaacatc aacgtggaag ccagcaagaa taagagcaaa acctcaacaa
301agttgcatgt gggcaacatc agtcccacct gcaccaataa ggagcttcga gccaagtttg
361aggagtatgg tccggtcatc gaatgtgaca tcgtgaaaga ttatgccttc gtacacatgg
421agcgggcaga ggatgcagtg gaggccatca ggggccttga taacacagag tttcaaggca
481aacgaatgca cgtgcagttg tccaccagcc ggcttaggac tgcgcccggg atgggagacc
541agagcggctg ctatcggtgc gggaaagagg ggcactggtc caaagagtgt ccgatagatc
601gttcaggccg cgtggcagac ttgaccgagc aatataatga gcaatacgga gcagtgcgta
661cgccttacac catgagctat ggggattcat tgtattacaa caacgcgtac ggagcgctcg
721atgccTACTA CAAGCGCTGC CGTGCTGCCC GGTCCTATGA GGCAGTGGCA GCTGCAGCTG
781CCTCCGTGTA TAATTACGCA GAGCAGACCC TGTCCCAGCT GCCACAAGTC CAGAATACAG
841CCATGGCCAG TCACCTCACC TCCACCTCTC TCGATCCCTA CGATAGACAC CTGTTGCCGA
901CCTCAGGAGC TGCTGCCACA GCTGCTGCTG CAGCAGCAGC CGCTGCTGCT GTTACTGCAG
961CTTCCACTTC ATATTACGGG CGGGATCGGA GCCCCCTGCG TCGCGCTACA GCCCCAGTCC
1021CCACTGTTGG AGAGGGCTAC GGTTACGGGC ATGAGAGTGA GTTGTCCCAA GCTTCAGCAG
1081CCGCGCGGAA TTCTCTGTAC GACATGGCCC GGTATGAGCG GGAGCAGTAT GCCGATCGGG
1141CGCGGTACTC AGCCTTTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1201TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1261GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACATTGTC TACCGGTTTA
1321ACTCAATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)