Construct: ORF TRCN0000467637
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001236.1_s317c1
- Derived from:
- ccsbBroadEn_09125
- DNA Barcode:
- TGAGCAAGTGAAACAAACTGTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRO (83876)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467637
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83876 | MRO | maestro | NM_001369508.2 | 99.5% | 99.1% | 117G>C;400A>G;429C>T |
| 2 | human | 83876 | MRO | maestro | NM_001369510.2 | 99.5% | 99.1% | 117G>C;400A>G;429C>T |
| 3 | human | 83876 | MRO | maestro | NM_031939.6 | 99.5% | 99.1% | 117G>C;400A>G;429C>T |
| 4 | human | 83876 | MRO | maestro | XM_017026035.2 | 99.5% | 99.1% | 117G>C;400A>G;429C>T |
| 5 | human | 83876 | MRO | maestro | NM_001127176.3 | 88.3% | 80.2% | (many diffs) |
| 6 | human | 83876 | MRO | maestro | NM_001127174.3 | 78.6% | 78.2% | (many diffs) |
| 7 | human | 83876 | MRO | maestro | NM_001369511.2 | 78.6% | 78.2% | (many diffs) |
| 8 | human | 83876 | MRO | maestro | NM_001369515.2 | 78.6% | 78.2% | (many diffs) |
| 9 | human | 83876 | MRO | maestro | XM_011526226.3 | 78.6% | 78.2% | (many diffs) |
| 10 | human | 83876 | MRO | maestro | NM_001127175.3 | 68.8% | 61.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 810
- ORF length:
- 744
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccaaagacag aggagaatcc tgggccagcc cctttccatc cctacttccc 121 agcccaagca gaaaaggaca tcaatgatat ctttcttttc caaggtctct tggaaactga 181 gcttccagaa gcgggagcct ctgaagaatg tgtttttcat cttggcagaa agagctcggg 241 accccagtgc taaaaagcgt cacatggcaa tgagaaactt gggaaccatg gcctatgaag 301 cccctgacaa ggtgagaaag tataagaaaa ttgtcctcga cctgctggtg tatggactgt 361 atgaccctgt gaatttggaa gtcatccatg agagtatgaa gactctgacc gtcgttctgg 421 gcaagatcca ggggaaaggt ttgggttccT TCTTCATAGA TATCGCCCTT CAGACCAGGA 481 CTTTATTAGA TGATGAGAAC GACAGTCTGA GATACTCGGC CTTTGTTTTG TTTGGGCAAT 541 TGGCTGCCTT TGCCGGGAGG AAATGGAAAA AATTTTTCAC CAGTCAGGTT AAGCAGACAC 601 GAGATTCCCT CCTGATCCAT TTACAGGACA GAAATCCCCA GGTTGCCAAG GCTTGCAAAA 661 CAACATTTCA AGCCTGTTCT CCATATCTGA AACTAAAGGA GGAATACAGC TTCCAGAGTG 721 AAGAAGATCA AAGGAACACT AAGCTCTACC AGCAGCTGAG CCACTATCAT CCAGAGATCC 781 TGCAGTTCTT CTACGCAAAT AAAATTCTGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 841 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 901 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGAGC 961 AAGTGAAACA AACTGTCTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1021 tgaaagatt