Construct: ORF TRCN0000467651
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014610.3_s317c1
- Derived from:
- ccsbBroadEn_10423
- DNA Barcode:
- GCTTTACATTAAATTTCAAATCGT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMPR1A (657)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467651
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 657 | BMPR1A | bone morphogenetic protein ... | NM_004329.2 | 99.8% | 99.6% | 9G>A;1585G>C;1590delA |
| 2 | human | 657 | BMPR1A | bone morphogenetic protein ... | XM_011540103.2 | 99.8% | 99.6% | 9G>A;1585G>C;1590delA |
| 3 | human | 657 | BMPR1A | bone morphogenetic protein ... | XM_011540104.2 | 99.8% | 99.6% | 9G>A;1585G>C;1590delA |
| 4 | mouse | 12166 | Bmpr1a | bone morphogenetic protein ... | NM_009758.4 | 90.7% | 97.5% | (many diffs) |
| 5 | mouse | 12166 | Bmpr1a | bone morphogenetic protein ... | XM_006518469.4 | 90.7% | 97.5% | (many diffs) |
| 6 | mouse | 12166 | Bmpr1a | bone morphogenetic protein ... | XM_006518470.4 | 90.7% | 97.5% | (many diffs) |
| 7 | mouse | 12166 | Bmpr1a | bone morphogenetic protein ... | XM_017315806.2 | 90.7% | 97.5% | (many diffs) |
| 8 | mouse | 12166 | Bmpr1a | bone morphogenetic protein ... | XM_030247615.1 | 90.7% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1662
- ORF length:
- 1596
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tcaactatac atttacatca gattattggg agcctatttg ttcatcattt 121 ctcgtgttca aggacagaat ctggatagta tgcttcatgg cactgggatg aaatcagact 181 ccgaccagaa aaagtcagaa aatggagtaa ccttagcacc agaggatacc ttgccttttt 241 taaagtgcta ttgctcaggg cactgtccag atgatgctat taataacaca tgcataacta 301 atggacattg ctttgccatc atagaagaag atgaccaggg agaaaccaca ttagcttcag 361 ggtgtatgaa atatgaagga tctgattttc agtgcaaaga ttctccaaaa gcccagctac 421 gccggacaat agaatgttgt cggaccaatt tatgtaacca gtatttgcaa cccacactgc 481 cccctgttgt cataggtccg ttttttgatg gcagcattcg atggctggtt ttgctcattt 541 ctatggctgt ctgcataatt gctatgatca tcttctccag ctgcttttgt tacaaacatt 601 attgcaagag catctcaagc agacgtcgtt acaatcgtga tttggaacag gatgaagcat 661 ttattccagt tggagaatca ctaaaagacc ttattgacca gtcacaaagt tctggtagtg 721 ggtctggact acctttattg gttcagcgaa ctattgccaa acagattcag atggtccggc 781 aagttggtaa aggccgatat ggagaagtat ggatgggcaa atggcgtggc gaaaaagtgg 841 cggtgaaagt attctttacc actgaagaag ccagctggtt tcgagaaaca gaaatctacc 901 aaactgtgct aatgcgccat gaaaacatac ttggtttcat agcggcagac attaaaggta 961 caggttcctg gactcagctc tatttgatta ctgattacca tgaaaatgga tctctctatg 1021 acttcctgaa atgtgctaca ctggacacca gagccctgct taaattggct tattcagctg 1081 cctgtggtct gtgccacctg cacacagaaa tttatggcac ccaaggaaag cccgcaattg 1141 ctcatcgaga cctaaagagc aaaaacatcc tcatcaagaa aaatgggagt tgctgcattg 1201 ctgacctggg ccttgctgtt aaattcaaca gtgacacaaa tgaagttgat gtgcccttGA 1261 ATACCAGGGT GGGCACCAAA CGCTACATGG CTCCCGAAGT GCTGGACGAA AGCCTGAACA 1321 AAAACCACTT CCAGCCCTAC ATCATGGCTG ACATCTACAG CTTCGGCCTA ATCATTTGGG 1381 AGATGGCTCG TCGTTGTATC ACAGGAGGGA TCGTGGAAGA ATACCAATTG CCATATTACA 1441 ACATGGTACC GAGTGATCCG TCATACGAAG ATATGCGTGA GGTTGTGTGT GTCAAACGTT 1501 TGCGGCCAAT TGTGTCTAAT CGGTGGAACA GTGATGAATG TCTACGAGCA GTTTTGAAGC 1561 TAATGTCAGA ATGCTGGGCC CACAATCCAG CCTCCAGACT CACAGCATTG AGAATTAAGA 1621 AGACGCTTGC CAAGATGGTT GAATCCCAAC ATGTAAAATC TGCCCAACTT TCTTGTACAA 1681 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 1741 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 1801 GGACGAGCTT TACATTAAAT TTCAAATCGT ACGCGTTAAG TCgacaatca acctctggat 1861 tacaaaattt gtgaaagatt