Construct: ORF TRCN0000467660
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008176.1_s317c1
- Derived from:
- ccsbBroadEn_01463
- DNA Barcode:
- GACATATACGATGGCGTATCTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS27A (6233)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467660
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6233 | RPS27A | ribosomal protein S27a | NM_001135592.2 | 100% | 100% | |
| 2 | human | 6233 | RPS27A | ribosomal protein S27a | NM_001177413.1 | 100% | 100% | |
| 3 | human | 6233 | RPS27A | ribosomal protein S27a | NM_002954.5 | 100% | 100% | |
| 4 | human | 7314 | UBB | ubiquitin B | NM_018955.4 | 48.9% | 24.6% | (many diffs) |
| 5 | human | 7316 | UBC | ubiquitin C | NM_021009.7 | 18% | 9.9% | (many diffs) |
| 6 | human | 100616345 | MIR4426 | microRNA 4426 | NR_039624.1 | 13.2% | 0_1ins145;3T>C;63_64ins260 | |
| 7 | mouse | 78294 | Rps27a | ribosomal protein S27A | NM_001033865.1 | 92.5% | 99.3% | (many diffs) |
| 8 | mouse | 78294 | Rps27a | ribosomal protein S27A | NM_024277.2 | 92.5% | 99.3% | (many diffs) |
| 9 | mouse | 664894 | Gm13215 | predicted gene 13215 | XR_104891.4 | 82.1% | (many diffs) | |
| 10 | mouse | 667035 | Gm8430 | predicted pseudogene 8430 | XR_869418.1 | 69.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 534
- ORF length:
- 468
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gattttcgtg aaaaccctta cggggaagac catcaccctc gaggttgaac 121 cctcggatac gatagaaaat gtaaaggcca agatccagga taaggaagga attcctcctg 181 atcagcagag actgatcttt gctggcaagc agctggaaga tggacgtact tTGTCTGACT 241 ACAATATTCA AAAGGAGTCT ACTCTTCATC TTGTGTTGAG ACTTCGTGGT GGTGCTAAGA 301 AAAGGAAGAA GAAGTCTTAC ACCACTCCCA AGAAGAATAA GCACAAGAGA AAGAAGGTTA 361 AGCTGGCTGT CCTGAAATAT TATAAGGTGG ATGAGAATGG CAAAATTAGT CGCCTTCGTC 421 GAGAGTGCCC TTCTGATGAA TGTGGTGCTG GGGTGTTTAT GGCAAGTCAC TTTGACAGAC 481 ATTATTGTGG CAAATGTTGT CTGACTTACT GTTTCAACAA ACCAGAAGAC AAGTACCCAA 541 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 601 ATTCTACGTA GTAATGAACT AGTCCGTAAC TGAAAGTATT TCGATTTCTT GGCTTTATAT 661 ATCTTGTGGA AAGGACGAGA CATATACGAT GGCGTATCTA TAACGCGTTA AGTCgacaat 721 caacctctgg attacaaaat ttgtgaaaga tt