Transcript: Human NM_021009.7

Homo sapiens ubiquitin C (UBC), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
UBC (7316)
Length:
2193
CDS:
68..2125

Additional Resources:

NCBI RefSeq record:
NM_021009.7
NBCI Gene record:
UBC (7316)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_021009.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423348 CTCAGAGGTGGGATGCAAATC pLKO_005 284 CDS 100% 10.800 14.040 N UBC n/a
2 TRCN0000011111 GAGGTTGATCTTTGCCGGAAA pLKO.1 418 CDS 100% 4.050 5.265 N UBC n/a
3 TRCN0000011109 CGAGAACGTCAAAGCAAAGAT pLKO.1 364 CDS 100% 5.625 3.938 N UBC n/a
4 TRCN0000011107 CGAGAATGTCAAGGCAAAGAT pLKO.1 136 CDS 100% 5.625 3.938 N UBC n/a
5 TRCN0000426019 ACGCACCCTGTCTGACTACAA pLKO_005 1366 CDS 100% 4.950 3.465 N UBC n/a
6 TRCN0000412861 CGTACCCTGTCTGACTACAAC pLKO_005 683 CDS 100% 4.950 3.465 N UBC n/a
7 TRCN0000011108 GCAAAGATCCAAGACAAGGAA pLKO.1 833 CDS 100% 3.000 2.100 N UBC n/a
8 TRCN0000272013 CCAGTGACACCATCGAGAATG pLKO_005 123 CDS 100% 10.800 6.480 N Gm11808 n/a
9 TRCN0000433566 GAAGACCCTGACTGGTAAGAC pLKO_005 538 CDS 100% 4.950 2.970 N UBC n/a
10 TRCN0000434069 GCCGAGTGACACCATTGAGAA pLKO_005 1033 CDS 100% 4.950 2.970 N UBC n/a
11 TRCN0000011110 AGGTTGATCTTTGCTGGGAAA pLKO.1 1331 CDS 100% 4.050 2.430 N UBC n/a
12 TRCN0000433586 TGGGATGCAGATCTTCGTGAA pLKO_005 520 CDS 100% 4.050 2.430 N UBC n/a
13 TRCN0000086705 GATCCAAGACAAGGAAGGCAT pLKO.1 838 CDS 100% 2.640 1.584 N LOC333475 n/a
14 TRCN0000086860 GCCCAGTGACACCATCGAGAA pLKO.1 121 CDS 100% 1.350 0.810 N Gm1821 n/a
15 TRCN0000098656 CCATTGAGAATGTCAAGGCAA pLKO.1 1044 CDS 100% 2.640 1.320 Y Ubc n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_021009.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14873 pDONR223 70.1% 29.4% 33.2% None (many diffs) n/a
2 ccsbBroad304_14873 pLX_304 0% 29.4% 33.2% V5 (many diffs) n/a
3 ccsbBroadEn_14872 pDONR223 58.4% 29.1% 33.2% None (many diffs) n/a
4 ccsbBroad304_14872 pLX_304 0% 29.1% 33.2% V5 (many diffs) n/a
5 TRCN0000468005 TCGGGCCTGTCTTATCTTATATCC pLX_317 100% 9.7% 11% V5 (many diffs) n/a
6 ccsbBroadEn_01463 pDONR223 100% 18% 9.9% None (many diffs) n/a
7 ccsbBroad304_01463 pLX_304 0% 18% 9.9% V5 (many diffs) n/a
8 TRCN0000467660 GACATATACGATGGCGTATCTATA pLX_317 64.1% 18% 9.9% V5 (many diffs) n/a
9 ccsbBroadEn_01732 pDONR223 100% 15.1% 10.3% None (many diffs) n/a
10 ccsbBroad304_01732 pLX_304 0% 15.1% 10.3% V5 (many diffs) n/a
11 TRCN0000479679 GCATCGCTGTTGTTTGACTCTTAG pLX_317 87.9% 15.1% 10.3% V5 (many diffs) n/a
Download CSV