Transcript: Mouse NM_024277.2

Mus musculus ribosomal protein S27A (Rps27a), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Rps27a (78294)
Length:
726
CDS:
218..688

Additional Resources:

NCBI RefSeq record:
NM_024277.2
NBCI Gene record:
Rps27a (78294)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_024277.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000095035 GCATAAGAGGAAGAAGGTTAA pLKO.1 493 CDS 100% 10.800 5.400 Y Rps27a n/a
2 TRCN0000095038 GAGAGTGTCCTTCTGATGAAT pLKO.1 573 CDS 100% 5.625 2.813 Y Rps27a n/a
3 TRCN0000095034 CCGGACTTTGTCTGACTACAA pLKO.1 376 CDS 100% 4.950 2.475 Y Rps27a n/a
4 TRCN0000095037 GAGGCTGATCTTTGCTGGTAA pLKO.1 340 CDS 100% 4.950 2.475 Y Rps27a n/a
5 TRCN0000007187 GCCAAGATCCAGGATAAGGAA pLKO.1 299 CDS 100% 3.000 1.500 Y RPS27A n/a
6 TRCN0000273406 GCCAAGATCCAGGATAAGGAA pLKO_005 299 CDS 100% 3.000 1.500 Y RPS27A n/a
7 TRCN0000095036 CTGAAATACTATAAGGTGGAT pLKO.1 524 CDS 100% 2.640 1.320 Y Rps27a n/a
8 TRCN0000007190 GAAGTCTTACACCACTCCCAA pLKO.1 463 CDS 100% 2.640 1.320 Y RPS27A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024277.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01463 pDONR223 100% 92.5% 99.3% None (many diffs) n/a
2 ccsbBroad304_01463 pLX_304 0% 92.5% 99.3% V5 (many diffs) n/a
3 TRCN0000467660 GACATATACGATGGCGTATCTATA pLX_317 64.1% 92.5% 99.3% V5 (many diffs) n/a
4 TRCN0000468005 TCGGGCCTGTCTTATCTTATATCC pLX_317 100% 40.9% 48.7% V5 (many diffs) n/a
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