Construct: ORF TRCN0000467690
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011480.1_s317c1
- Derived from:
- ccsbBroadEn_00427
- DNA Barcode:
- TACACGGGCCCTAACCAGGCATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCT (1638)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467690
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1638 | DCT | dopachrome tautomerase | NM_001922.5 | 100% | 100% | |
| 2 | human | 1638 | DCT | dopachrome tautomerase | NM_001129889.2 | 94% | 94% | 1178_1276del |
| 3 | human | 1638 | DCT | dopachrome tautomerase | XM_017020401.2 | 92.3% | 92.3% | 1179_1307del |
| 4 | human | 1638 | DCT | dopachrome tautomerase | NM_001322186.2 | 85.4% | 82.6% | (many diffs) |
| 5 | human | 1638 | DCT | dopachrome tautomerase | XM_011521049.2 | 85.4% | 82.6% | (many diffs) |
| 6 | human | 1638 | DCT | dopachrome tautomerase | NM_001322182.2 | 40% | 40% | 0_1ins933 |
| 7 | human | 1638 | DCT | dopachrome tautomerase | NM_001322183.2 | 40% | 40% | 0_1ins933 |
| 8 | human | 1638 | DCT | dopachrome tautomerase | NM_001322184.2 | 40% | 40% | 0_1ins933 |
| 9 | human | 1638 | DCT | dopachrome tautomerase | NM_001322185.2 | 40% | 40% | 0_1ins933 |
| 10 | human | 1638 | DCT | dopachrome tautomerase | XM_024449328.1 | 40% | 40% | 0_1ins933 |
| 11 | human | 1638 | DCT | dopachrome tautomerase | XR_002957452.1 | 34.8% | 1_237del;1416_1488del;1868_4464del | |
| 12 | mouse | 13190 | Dct | dopachrome tautomerase | XM_006518510.3 | 51.6% | 52.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1623
- ORF length:
- 1557
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ccccctttgg tgggggtttc tgctcagttg cttgggctgc aaaatcctgc 121 caggagccca gggtcagttc ccccgagtct gcatgacggt ggacagccta gtgaacaagg 181 agtgctgccc acgcctgggt gcagagtcgg ccaatgtctg tggctctcag caaggccggg 241 ggcagtgcac agaggtgcga gccgacacaa ggccctggag tggtccctac atcctacgaa 301 accaggatga ccgtgagctg tggccaagaa aattcttcca ccggacctgc aagtgcacag 361 gaaactttgc cggctataat tgtggagact gcaagtttgg ctggaccggt cccaactgcg 421 agcggaagaa accaccagtg attcggcaga acatccattc cttgagtcct caggaaagag 481 agcagttctt gggcgcctta gatctcgcga agaagagagt acaccccgac tacgtgatca 541 ccacacaaca ctggctgggc ctgcttgggc ccaatggaac ccagccgcag tttgccaact 601 gcagtgttta tgattttttt gtgtggctcc attattattc tgttagagat acattattag 661 gaccaggacg cccctacagg gccatagatt tctcacatca aggacctgca tttgttacct 721 ggcaccggta ccatttgttg tgtctggaaa gagatctcca gcgactcatt ggcaatgagt 781 cttttgcttt gccctactgg aactttgcca ctgggaggaa cgagtgtgat gtgtgtacag 841 accagctgtt tggggcagcg agaccagacg atccgactct gattagtcgg aactcaagat 901 tctccagctg ggaaactgtc tgtgatagct tggatgacta caaccacctg gtcaccttgt 961 gcaatggaac ctatgaaggt ttgctgagaa gaaatcaaat gggaagaaac agcatgaaat 1021 tgccaacctt aaaagacata cgagattgcc tgtctctcca gaagtttgac aatcctccct 1081 tcttccagaa ctctaccttc agtttcagga atgctttgga agggtttgat aaagcagatg 1141 ggactctgga ttctcaagtg atgagccttc ataatttggt tcattccttc ctgaacggga 1201 caaacgcttt gccacattca gccgccaatg atcccatttt tgtggttctt cattccttta 1261 ctgatgccat ctttGATGAG TGGATGAAAA GATTTAATCC TCCTGCAGAT GCCTGGCCTC 1321 AGGAGCTGGC CCCTATTGGT CACAATCGGA TGTACAACAT GGTTCCTTTC TTCCCTCCAG 1381 TGACTAATGA AGAACTCTTT TTAACCTCAG ACCAACTTGG CTACAGCTAT GCCATCGATC 1441 TGCCAGTTTC AGTTGAAGAA ACTCCAGGTT GGCCCACAAC TCTCTTAGTA GTCATGGGAA 1501 CACTGGTGGC TTTGGTTGGT CTTTTTGTGC TGTTGGCTTT TCTTCAATAT AGAAGACTTC 1561 GAAAAGGATA TACACCCCTA ATGGAGACAC ATTTAAGCAG CAAGAGATAC ACAGAAGAAG 1621 CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1681 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1741 GGCTTTATAT ATCTTGTGGA AAGGACGATA CACGGGCCCT AACCAGGCAT TTACGCGTTA 1801 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt