Construct: ORF TRCN0000467697
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006651.3_s317c1
- Derived from:
- ccsbBroadEn_14596
- DNA Barcode:
- CTGGGCCATGATGAACCTGATATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK14 (1432)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467697
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139012.2 | 100% | 100% | |
| 2 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_001315.2 | 94.6% | 96.1% | (many diffs) |
| 3 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010299.2 | 85.5% | 74.6% | (many diffs) |
| 4 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139014.2 | 85.2% | 70.4% | 762_763ins79;921_922ins80 |
| 5 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_011514310.3 | 83.9% | 82.2% | (many diffs) |
| 6 | human | 1432 | MAPK14 | mitogen-activated protein k... | NM_139013.3 | 81.3% | 77.5% | (many diffs) |
| 7 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010300.2 | 80.5% | 70.9% | (many diffs) |
| 8 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010301.2 | 79.8% | 77.3% | (many diffs) |
| 9 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_006714998.3 | 78.6% | 78.6% | 0_1ins231 |
| 10 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010304.2 | 70.8% | 70.6% | 764_767delATGC;772_773delCCinsAA;774_775ins310 |
| 11 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010302.2 | 68.3% | 61.7% | (many diffs) |
| 12 | human | 1432 | MAPK14 | mitogen-activated protein k... | XM_017010303.2 | 67.3% | 65.2% | (many diffs) |
| 13 | human | 1432 | MAPK14 | mitogen-activated protein k... | XR_926065.2 | 54.5% | (many diffs) | |
| 14 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_011951.3 | 90.2% | 99.4% | (many diffs) |
| 15 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168508.1 | 85.5% | 95.5% | (many diffs) |
| 16 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168513.1 | 71.5% | 78.3% | (many diffs) |
| 17 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | NM_001168514.1 | 71.5% | 78.3% | (many diffs) |
| 18 | mouse | 26416 | Mapk14 | mitogen-activated protein k... | XM_017317480.1 | 67.1% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1146
- ORF length:
- 1080
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcaggagagg cccacgttct accggcagga gctgaacaag acaatctggg 121 aggtgcccga gcgttaccag aacctgtctc cagtgggctc tggcgcctat ggctctgtgt 181 gtgctgcttt tgacacaaaa acggggttac gtgtggcagt gaagaagctc tccagaccat 241 ttcagtccat cattcatgcg aaaagaacct acagagaact gcggttactt aaacatatga 301 aacatgaaaa tgtgattggt ctgttggacg tttttacacc tgcaaggtct ctggaggaat 361 tcaatgatgt gtatctggtg acccatctca tgggggcaga tctgaacaac attgtgaaat 421 gtcagaagct tacagatgac catgttcagt tccttatcta ccaaattctc cgaggtctaa 481 agtatataca ttcagctgac ataattcaca gggacctaaa acctagtaat ctagctgtga 541 atgaagactg tgagctgaag attctggatt ttggactggc tcggcacaca gatgatgaaa 601 tgacaggcta cgtggccact aggtggtaca gggctcctga gatcatgctg aactggatgc 661 attacaacca gacagttgat atttggtcag tgggatgcat aatggccgag ctgttgactg 721 gaagaacatt gtttcctggt acagaccata ttgatcagtt gaagctcatt ttaagactcg 781 ttggaacccc aggggctgag cttttgaaga aaatctccTC AGAGTCTGCA AGAAACTATA 841 TTCAGTCTTT GACTCAGATG CCGAAGATGA ACTTTGCGAA TGTATTTATT GGTGCCAATC 901 CCCTGGCTGT CGACTTGCTG GAGAAGATGC TTGTATTGGA CTCAGATAAG AGAATTACAG 961 CGGCCCAAGC CCTTGCACAT GCCTACTTTG CTCAGTACCA CGATCCTGAT GATGAACCAG 1021 TGGCCGATCC TTATGATCAG TCCTTTGAAA GCAGGGACCT CCTTATAGAT GAGTGGAAAA 1081 GCCTGACCTA TGATGAAGTC ATCAGCTTTG TGCCACCACC CCTTGACCAA GAAGAGATGG 1141 AGTCCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1201 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1261 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTGGGCCAT GATGAACCTG ATATTACGCG 1321 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt