Transcript: Human NM_139013.3

Homo sapiens mitogen-activated protein kinase 14 (MAPK14), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-07-16
Taxon:
Homo sapiens (human)
Gene:
MAPK14 (1432)
Length:
1306
CDS:
351..1244

Additional Resources:

NCBI RefSeq record:
NM_139013.3
NBCI Gene record:
MAPK14 (1432)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148584 CAAGGCGAGTAATACCTGTC pXPR_003 TGG 608 68% 7 1.0123 MAPK14 MAPK14 77922
2 BRDN0001487162 AAGTAACCGCAGTTCTCTGT pXPR_003 AGG 208 23% 2 0.8869 MAPK14 MAPK14 77925
3 BRDN0001148417 TGATGAAATGACAGGCTACG pXPR_003 TGG 544 61% 7 -0.0667 MAPK14 MAPK14 77924
4 BRDN0001145809 CACAAAAACGGGGTTACGTG pXPR_003 TGG 145 16% 2 -0.5355 MAPK14 MAPK14 77923
Download CSV

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_139013.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000512 CGAGGTCTAAAGTATATACAT pLKO.1 756 CDS 100% 5.625 7.875 N MAPK14 n/a
2 TRCN0000010051 GTTACGTGTGGCAGTGAAGAA pLKO.1 491 CDS 100% 4.950 6.930 N MAPK14 n/a
3 TRCN0000196320 GCGGTTACTTAAACATATGAA pLKO.1 566 CDS 100% 0.000 0.000 N MAPK14 n/a
4 TRCN0000000510 CCATGTTCAGTTCCTTATCTA pLKO.1 725 CDS 100% 5.625 3.938 N MAPK14 n/a
5 TRCN0000000513 CCATTTCAGTCCATCATTCAT pLKO.1 522 CDS 100% 5.625 3.938 N MAPK14 n/a
6 TRCN0000055224 CTCAGAGTCTGCAAGAAACTA pLKO.1 1103 CDS 100% 5.625 3.938 N Mapk14 n/a
7 TRCN0000010054 CTCGGCACACAGATGATGAAA pLKO.1 865 CDS 100% 5.625 3.938 N MAPK14 n/a
8 TRCN0000023121 AGACCATATTGATCAGTTGAA pLKO.1 1028 CDS 100% 4.950 3.465 N Mapk14 n/a
9 TRCN0000194820 CCTAGTAATCTAGCTGTGAAT pLKO.1 807 CDS 100% 4.950 3.465 N MAPK14 n/a
10 TRCN0000010052 GTTCAGTTCCTTATCTACCAA pLKO.1 729 CDS 100% 3.000 2.100 N MAPK14 n/a
11 TRCN0000010053 GACATAATTCACAGGGACCTA pLKO.1 783 CDS 100% 2.640 1.848 N MAPK14 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_139013.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00371 pDONR223 100% 81.3% 77.5% None (many diffs) n/a
2 ccsbBroad304_00371 pLX_304 57.5% 81.3% 77.5% V5 (many diffs) n/a
3 ccsbBroadEn_14596 pDONR223 0% 81.3% 77.5% None (many diffs) n/a
4 ccsbBroad304_14596 pLX_304 57.5% 81.3% 77.5% V5 (many diffs) n/a
5 TRCN0000467697 CTGGGCCATGATGAACCTGATATT pLX_317 30.7% 81.3% 77.5% V5 (many diffs) n/a
6 TRCN0000488917 TATCTACGAACTTGGAGCTCCGGG pLX_317 31.3% 81.3% 77.5% V5 (many diffs) n/a
7 TRCN0000489126 CACCAATATGTCTGCCCACCGTGT pLX_317 30.7% 81.3% 77.5% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000488039 GGTTGATAAACGCGGGTAGCTCGT pLX_317 22.8% 81.3% 77.5% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000488445 GATCACTGAGCTGGTAGGCGGGCA pLX_317 30.7% 77.9% 81.8% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV