Transcript: Human XM_017010299.2

PREDICTED: Homo sapiens mitogen-activated protein kinase 14 (MAPK14), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAPK14 (1432)
Length:
1908
CDS:
407..1393

Additional Resources:

NCBI RefSeq record:
XM_017010299.2
NBCI Gene record:
MAPK14 (1432)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148584 CAAGGCGAGTAATACCTGTC pXPR_003 TGG 608 62% 7 0.9996 MAPK14 MAPK14 77922
2 BRDN0001487162 AAGTAACCGCAGTTCTCTGT pXPR_003 AGG 208 21% 2 0.9264 MAPK14 MAPK14 77925
3 BRDN0001148417 TGATGAAATGACAGGCTACG pXPR_003 TGG 544 55% 7 -0.0632 MAPK14 MAPK14 77924
4 BRDN0001145809 CACAAAAACGGGGTTACGTG pXPR_003 TGG 145 15% 2 -0.4903 MAPK14 MAPK14 77923
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017010299.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000512 CGAGGTCTAAAGTATATACAT pLKO.1 812 CDS 100% 5.625 7.875 N MAPK14 n/a
2 TRCN0000010051 GTTACGTGTGGCAGTGAAGAA pLKO.1 547 CDS 100% 4.950 6.930 N MAPK14 n/a
3 TRCN0000196320 GCGGTTACTTAAACATATGAA pLKO.1 622 CDS 100% 0.000 0.000 N MAPK14 n/a
4 TRCN0000000510 CCATGTTCAGTTCCTTATCTA pLKO.1 781 CDS 100% 5.625 3.938 N MAPK14 n/a
5 TRCN0000000513 CCATTTCAGTCCATCATTCAT pLKO.1 578 CDS 100% 5.625 3.938 N MAPK14 n/a
6 TRCN0000055224 CTCAGAGTCTGCAAGAAACTA pLKO.1 1159 CDS 100% 5.625 3.938 N Mapk14 n/a
7 TRCN0000010054 CTCGGCACACAGATGATGAAA pLKO.1 921 CDS 100% 5.625 3.938 N MAPK14 n/a
8 TRCN0000023121 AGACCATATTGATCAGTTGAA pLKO.1 1084 CDS 100% 4.950 3.465 N Mapk14 n/a
9 TRCN0000194820 CCTAGTAATCTAGCTGTGAAT pLKO.1 863 CDS 100% 4.950 3.465 N MAPK14 n/a
10 TRCN0000010052 GTTCAGTTCCTTATCTACCAA pLKO.1 785 CDS 100% 3.000 2.100 N MAPK14 n/a
11 TRCN0000010053 GACATAATTCACAGGGACCTA pLKO.1 839 CDS 100% 2.640 1.848 N MAPK14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017010299.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00371 pDONR223 100% 85.5% 74.6% None (many diffs) n/a
2 ccsbBroad304_00371 pLX_304 57.5% 85.5% 74.6% V5 (many diffs) n/a
3 ccsbBroadEn_14596 pDONR223 0% 85.5% 74.6% None (many diffs) n/a
4 ccsbBroad304_14596 pLX_304 57.5% 85.5% 74.6% V5 (many diffs) n/a
5 TRCN0000467697 CTGGGCCATGATGAACCTGATATT pLX_317 30.7% 85.5% 74.6% V5 (many diffs) n/a
6 TRCN0000488917 TATCTACGAACTTGGAGCTCCGGG pLX_317 31.3% 85.5% 74.6% V5 (many diffs) n/a
7 TRCN0000489126 CACCAATATGTCTGCCCACCGTGT pLX_317 30.7% 85.5% 74.6% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000488039 GGTTGATAAACGCGGGTAGCTCGT pLX_317 22.8% 85.5% 74.6% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000488445 GATCACTGAGCTGGTAGGCGGGCA pLX_317 30.7% 82.1% 74.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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