Construct: ORF TRCN0000467727
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011930.1_s317c1
- Derived from:
- ccsbBroadEn_08776
- DNA Barcode:
- CCCCTAATATCAATCCCCCCGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NLRC4 (58484)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467727
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58484 | NLRC4 | NLR family CARD domain cont... | NM_001199138.2 | 99.9% | 99.9% | 22A>G;3069T>C |
| 2 | human | 58484 | NLRC4 | NLR family CARD domain cont... | NM_001199139.1 | 99.9% | 99.9% | 22A>G;3069T>C |
| 3 | human | 58484 | NLRC4 | NLR family CARD domain cont... | NM_021209.4 | 99.9% | 99.9% | 22A>G;3069T>C |
| 4 | human | 58484 | NLRC4 | NLR family CARD domain cont... | XR_001738872.1 | 84% | (many diffs) | |
| 5 | human | 58484 | NLRC4 | NLR family CARD domain cont... | XM_017004619.1 | 76.5% | 76.3% | (many diffs) |
| 6 | human | 58484 | NLRC4 | NLR family CARD domain cont... | NM_001302504.1 | 34.9% | 34.9% | 22A>G;262_263ins1995;1074T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3138
- ORF length:
- 3072
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tttcataaag gacaatggcc gagcccttat tcaaagaatg ggaatgactg 121 ttataaagca aatcacagat gacctatttg tatggaatgt tctgaatcgc gaagaagtaa 181 acatcatttg ctgcgagaag gtggagcagg atgctgctag agggatcatt cacatgattt 241 tgaaaaaggg ttcagagtcc tgtaacctct ttcttaaatc ccttaaggag tggaactatc 301 ctctatttca ggacttgaat ggacaaagtc tttttcatca gacatcagaa ggagacttgg 361 acgatttggc tcaggattta aaggacttgt accatacccc atcttttctg aacttttatc 421 cccttggtga agatattgac attattttta acttgaaaag caccttcaca gaacctgtcc 481 tgtggaggaa ggaccaacac catcaccgcg tggagcagct gaccctgaat ggcctcctgc 541 aggctcttca gagcccctgc atcattgaag gggaatctgg caaaggcaag tccactctgc 601 tgcagcgaat tgccatgctc tggggctccg gaaagtgcaa ggctctgacc aagttcaaat 661 tcgtcttctt cctccgtctc agcagggccc agggtggact ttttgaaacc ctctgtgatc 721 aactcctgga tatacctggc acaatcagga agcagacatt catggccatg ctgctgaagc 781 tgcggcagag ggttcttttc cttcttgatg gctacaatga attcaagccc cagaactgcc 841 cagaaatcga agccctgata aaggaaaacc accgcttcaa gaacatggtc atcgtcacca 901 ctaccactga gtgcctgagg cacatacggc agtttggtgc cctgactgct gaggtggggg 961 atatgacaga agacagcgcc caggctctca tccgagaagt gctgatcaag gagcttgctg 1021 aaggcttgtt gctccaaatt cagaaatcca ggtgcttgag gaatctcatg aagacccctc 1081 tctttgtggt catcacttgt gcaatccaga tgggtgaaag tgagttccac tctcacacac 1141 aaacaacgct gttccatacc ttctatgatc tgttgataca gaaaaacaaa cacaaacata 1201 aaggtgtggc tgcaagtgac ttcattcgga gcctggacca ctgtggagac ctagctctgg 1261 agggtgtgtt ctcccacaag tttgatttcg aactgcagga tgtgtccagc gtgaatgagg 1321 atgtcctgct gacaactggg ctcctctgta aatatacagc tcaaaggttc aagccaaagt 1381 ataaattctt tcacaagtca ttccaggagt acacagcagg acgaagactc agcagtttat 1441 tgacgtctca tgagccagag gaggtgacca aggggaatgg ttacttgcag aaaatggttt 1501 ccatttcgga cattacatcc acttatagca gcctgctccg gtacacctgt gggtcatctg 1561 tggaagccac cagggctgtt atgaagcacc tcgcagcagt gtatcaacac ggctgccttc 1621 tcggactttc catcgccaag aggcctctct ggagacagga atctttgcaa agtgtgaaaa 1681 acaccactga gcaagaaatt ctgaaagcca taaacatcaa ttcctttgta gagtgtggca 1741 tccatttata tcaagagagt acatccaaat cagccctgag ccaagaattt gaagctttct 1801 ttcaaggtaa aagcttatat atcaactcag ggaacatccc cgattactta tttgacttct 1861 ttgaacattt gcccaattgt gcaagtgccc tggacttcat taaactggac ttttatgggg 1921 gagctatggc ttcatgggaa aaggctgcag aagacacagg tggaatccac atggaagagg 1981 ccccagaaac ctacattccc agcagggctg tatctttgtt cttcaactgg aagcaggaat 2041 tcaggactct ggaggtcaca ctccgggatt tcagcaagtt gaataagcaa gatatcagat 2101 atctggggaa aatattcagc tctgccacaa gcctcaggct gcaaataaag agatgtgctg 2161 gtgtggctgg aagcctcagt ttggtcctca gcacctgtaa gaacatttat tctctcatgg 2221 tggaagccag tcccctcacc atagaagatg agaggcacat cacatctgta acaaacctga 2281 aaaccttgag tattcatgac ctacagaatc aacggctgcc gggtggtctg actgacagct 2341 tgggtaactt gaagaacctt acaaagctca taatggataa cataaagatg aatgaagaag 2401 atgctataaa actagctgaa ggcctgaaaa acctgaagaa gatgtgttta tttcatttga 2461 cccacttgtc tgacattgga gagggaatgg attacatagt caagtctctg tcaagtgaac 2521 cctgtgacct tgaagaaatt caattagtct cctgctgctt gtctgcaaat gcagtgaaaa 2581 tcctagctca gaatcttcac aatttggtca aactgagcat tcttgattta tcagaaaatt 2641 acctggaaaa agatggaaat gaagctcttc atgaactgat cgacaggatg aacgtgctag 2701 aacagctcac cgcactgatg ctgccctggg gctgtgacgt gcaaggcagc cTGAGCAGCC 2761 TGTTGAAACA TTTGGAGGAG GTCCCACAAC TCGTCAAGCT TGGGTTGAAA AACTGGAGAC 2821 TCACAGATAC AGAGATTAGA ATTTTAGGTG CATTTTTTGG AAAGAACCCT CTGAAAAACT 2881 TCCAGCAGTT GAATTTGGCG GGAAATCGTG TGAGCAGTGA TGGATGGCTT GCCTTCATGG 2941 GTGTATTTGA GAATCTTAAG CAATTAGTGT TTTTTGACTT TAGTACTAAA GAATTTCTAC 3001 CTGATCCAGC ATTAGTCAGA AAACTTAGCC AAGTGTTATC CAAGTTAACT TTTCTGCAAG 3061 AAGCTAGGCT TGTTGGGTGG CAATTTGATG ATGATGATCT CAGTGTTATT ACAGGTGCTT 3121 TTAAACTAGT AACCGCTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3181 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCC GTAACTTGAA 3241 AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCCCTA ATATCAATCC 3301 CCCCGCTCAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt