Construct: ORF TRCN0000467741

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002548.1_s317c1
Derived from:
ccsbBroadEn_03521
DNA Barcode:
TCCACCTAGTCGGGTTCTTTAGTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SAMD4B (55095)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467741
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55095 SAMD4B sterile alpha motif domain ... NM_018028.3 100% 100%
2 human 55095 SAMD4B sterile alpha motif domain ... XM_011527063.2 100% 100%
3 human 55095 SAMD4B sterile alpha motif domain ... XM_017026920.1 100% 100%
4 human 55095 SAMD4B sterile alpha motif domain ... XM_017026921.1 100% 100%
5 human 55095 SAMD4B sterile alpha motif domain ... XM_017026922.2 99.1% 97.7% (many diffs)
6 human 55095 SAMD4B sterile alpha motif domain ... XM_017026923.2 99.1% 97.7% (many diffs)
7 human 55095 SAMD4B sterile alpha motif domain ... NM_001303614.1 99.1% 98.7% (many diffs)
8 human 55095 SAMD4B sterile alpha motif domain ... XM_011527064.2 99.1% 98.7% (many diffs)
9 human 55095 SAMD4B sterile alpha motif domain ... XM_011527057.2 97.4% 97.1% (many diffs)
10 human 55095 SAMD4B sterile alpha motif domain ... XM_011527060.3 97.4% 97.1% (many diffs)
11 human 55095 SAMD4B sterile alpha motif domain ... XM_011527061.3 97.4% 97.1% (many diffs)
12 human 55095 SAMD4B sterile alpha motif domain ... XM_011527062.2 97.4% 97.1% (many diffs)
13 human 55095 SAMD4B sterile alpha motif domain ... XM_017026918.1 97.4% 97.1% (many diffs)
14 human 55095 SAMD4B sterile alpha motif domain ... XM_017026919.1 97.4% 97.1% (many diffs)
15 mouse 233033 Samd4b sterile alpha motif domain ... NM_175021.3 92% 96.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2148
ORF length:
2082
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat gttccgagac caggtgggca tcctcgctgg ctggttcaaa ggctggaatg
121agtgtgagca gacagtggcc ctcctgtcac ttctgaaacg ggtcacccgt acccaggccc
181gcttcctgca gctctgcctg gagcactcac tggcggactg caatgacatc cacctgctgg
241agtcggaggc caacagtgct gccatcgtca gccagtggca gcaggagtcc aaagagaagg
301tggtgtccct cctgctgtcc caccttcccc tgcttcagcc aggcaacaca gaggccaagt
361cggagtacat gaggctactg cagaaagtgc tggcctactc aatcgagagc aatgctttca
421tcgaggagag tcgccagctg ctttcctatg ccctcatcca cccagccacc acactggagg
481accgcaacgc actggccctc tggctgagcc acctggaaga gcggttggct agtggcttcc
541gctcccggcc agagccctcc taccattcac gtcaaggctc agatgagtgg gggggccctg
601cagagctagg ccctggggag gcagggccag gctggcagga caagccaccc cgggaaaatg
661gacacgtgcc cttccaccca tccagctcag tgccgccagc catcaacagt attgggagca
721atgcaaacac aggtctcccc tgccaaatcc accctagccc actgaagcgc tccatgtcac
781tcatccctac aagcccccag gtccctggtg agtggccgag tccagaggag cttggggccc
841gggctgcttt taccacgccc gatcacgcac ctctctcgcc ccagagcagc gtggcctcct
901ctggcagtga gcagacagag gagcagggct ccagccggaa caccttccag gaggatggca
961gtggcatgaa agatgtgccc tcatggctca agagcctccg tttgcacaag tatgcagccc
1021tcttctcaca gatgagctac gaggagatga tgacactgac tgagcagcac ctggagtctc
1081agaacgtcac caaaggtgcc cgccacaaga tagccctgag catccagaag ctgcgtgaga
1141gacagagcgt cctcaagtcc ctagagaagg atgtgctgga aggcgggaac ctacgaaacg
1201ctctgcagga gctgcagcag atcatcatca ctcccatcaa ggcctacagt gtcctccagg
1261ccaccgtggc tgccgccacc accaccccta ctgccaagga tggggccccg ggggaaccac
1321cgctgccagg tgctgagcct cccctagccc accccggcac agacaaaggc accgaggcca
1381aggaccctcc agctgtggag aactacccac ctccaccagc tccagctccc actgatggca
1441gtgagcctgc cccggctccc gtcgccgacg gagacatccc cagccagttt acacgggtga
1501tgggcaaagt gtgcacccaa ctgctggtgt cccgaccaga cgaggagaac atcaccagtt
1561acctccagct catcgaaaag tgcctgactc atgaggcttt cacggagacg cagaagaaac
1621ggctgctatc ctggaaacag caagtgctga agctcctccg gacattcccg cgcaaagccg
1681cactagagat gcagaactac cggcagcaga aaggctgggc attcggctcc aactcgctcc
1741ccatagctgg ctctgtgggg atgggagtgg cccggcgtac ccagcggcag ttcccaatgc
1801ctccccgggc cctcccaccc ggccggatgg gcctccTGAG CCCCTCGGGC ATTGGGGGTG
1861TCTCCCCTCG ACATGCCCTC ACCAGCCCCA GCCTTGGAGG CCAGGGCCGA CAGAACCTGT
1921GGTTTGCCAA CCCTGGAGGC AGCAACAGCA TGCCCAGTCA GAGCCGCAGC TCTGTGCAGC
1981GCACCCACTC GCTCCCGGTC CACTCGTCAC CCCAGGCCAT TCTCATGTTC CCTCCAGACT
2041GCCCGGTTCC TGGGCCTGAC CTGGAGATCA ATCCCACTCT GGAGTCTCTG TGTCTGAGCA
2101TGACAGAACA CGCCTTGGGT GATGGGACAG ACAAAACCTC CACCATCTGC CCAACTTTCT
2161TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
2221CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
2281GTGGAAAGGA CGATCCACCT AGTCGGGTTC TTTAGTGACG CGTTAAGTCg acaatcaacc
2341tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)