Transcript: Human XM_011527061.3

PREDICTED: Homo sapiens sterile alpha motif domain containing 4B (SAMD4B), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SAMD4B (55095)
Length:
2529
CDS:
251..2380

Additional Resources:

NCBI RefSeq record:
XM_011527061.3
NBCI Gene record:
SAMD4B (55095)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011527061.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000005451 CGCACTAGAGATGCAGAACTA pLKO.1 1864 CDS 100% 4.950 6.930 N SAMD4B n/a
2 TRCN0000189655 CGAGGAGAACATCACCAGTTA pLKO.1 1726 CDS 100% 4.950 3.960 N Samd4b n/a
3 TRCN0000314326 CGAGGAGAACATCACCAGTTA pLKO_005 1726 CDS 100% 4.950 3.960 N Samd4b n/a
4 TRCN0000005452 GAGTCTCAGAACGTCACCAAA pLKO.1 1259 CDS 100% 4.950 3.465 N SAMD4B n/a
5 TRCN0000005449 GCCTACTCAATCGAGAGCAAT pLKO.1 578 CDS 100% 4.950 3.465 N SAMD4B n/a
6 TRCN0000005450 GCATGAAAGATGTGCCCTCAT pLKO.1 1149 CDS 100% 4.050 2.835 N SAMD4B n/a
7 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 25 5UTR 100% 5.625 2.813 Y KLHL30 n/a
8 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 25 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011527061.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03521 pDONR223 100% 97.4% 97.1% None (many diffs) n/a
2 ccsbBroad304_03521 pLX_304 0% 97.4% 97.1% V5 (many diffs) n/a
3 TRCN0000467741 TCCACCTAGTCGGGTTCTTTAGTG pLX_317 15.3% 97.4% 97.1% V5 (many diffs) n/a
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