Construct: ORF TRCN0000467751
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014878.1_s317c1
- Derived from:
- ccsbBroadEn_14439
- DNA Barcode:
- CACCAGGAAGTGCCCACGCTTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OPN5 (221391)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467751
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221391 | OPN5 | opsin 5 | XM_017010415.1 | 77.3% | 76.9% | 101T>C;562_567delGACAAAinsT;572_729delinsA |
| 2 | human | 221391 | OPN5 | opsin 5 | XM_017010412.1 | 65.9% | 65.6% | (many diffs) |
| 3 | human | 221391 | OPN5 | opsin 5 | XM_017010413.1 | 65.9% | 65.6% | (many diffs) |
| 4 | human | 221391 | OPN5 | opsin 5 | XM_017010414.1 | 63.6% | 53.3% | (many diffs) |
| 5 | human | 221391 | OPN5 | opsin 5 | XM_017010411.1 | 63.3% | 63% | 1_327del;428T>C |
| 6 | human | 221391 | OPN5 | opsin 5 | XM_017010409.1 | 62% | 61.7% | (many diffs) |
| 7 | human | 221391 | OPN5 | opsin 5 | XM_017010410.1 | 56.3% | 44.8% | (many diffs) |
| 8 | human | 221391 | OPN5 | opsin 5 | NM_181744.3 | 53.2% | 53.1% | 1_495del;596T>C |
| 9 | human | 221391 | OPN5 | opsin 5 | XM_017010416.1 | 46% | 45.8% | (many diffs) |
| 10 | human | 221391 | OPN5 | opsin 5 | NR_033806.2 | 16.1% | 1_540del;641T>C;1108_3500del | |
| 11 | mouse | 353344 | Opn5 | opsin 5 | XM_006524453.1 | 48.4% | 51.3% | (many diffs) |
| 12 | mouse | 353344 | Opn5 | opsin 5 | NM_181753.4 | 43.6% | 46.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 636
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccttggta ggtctggggg actacgtacc tgagcccttc ggaacctcgt 121 gcaccctgga ctggtggctg gcccaggcct cggtaggggg ccaggtttcc atcctgaaca 181 tcctcttctt ctgcctcttg ctcccaacgg ctgtgatcgt gttctcctac gtaaagatca 241 ttgccaaggt taagtccTCT TCCAAAGAAG TAGCTCATTT CGACAGTCGG ATCCATAGCA 301 GCCATGTGCT GGAAATGAAA CTGACAAAGG TAGCGATGTT GATTTGTGCT GGATTCCTGA 361 TTGCCTGGAT TCCTTATGCA GTGGTGTCTG TGTGGTCAGC TTTTGGAAGG CCAGACTCCA 421 TTCCCATACA GCTCTCTGTG GTGCCAACCC TACTTGCAAA ATCTGCAGCG ATGTACAATC 481 CCATCATTTA CCAAGTTATT GATTACAAAT TTGCCTGTTG CCAAACTGGT GGTTTGAAAG 541 CAACCAAGAA GAAGTCTCTG GAAGGCTTCA GGCTGCACAC CGTAACCACA GTCAGGAAGT 601 CTTCTGCTGT GCTGGAAATT CATGAAGAGT GGGAATTGCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 ACACCAGGAA GTGCCCACGC TTTCAACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt