Construct: ORF TRCN0000467806
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014993.1_s317c1
- Derived from:
- ccsbBroadEn_16073
- DNA Barcode:
- CGGAATTGGTGCCGATAATAGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX18 (112574)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467806
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112574 | SNX18 | sorting nexin 18 | NM_001102575.2 | 99.8% | 100% | 270T>C;612T>C |
| 2 | human | 112574 | SNX18 | sorting nexin 18 | NM_001145427.2 | 90.5% | 83.6% | (many diffs) |
| 3 | human | 112574 | SNX18 | sorting nexin 18 | NM_052870.3 | 88.9% | 84% | (many diffs) |
| 4 | human | 112574 | SNX18 | sorting nexin 18 | XM_017008997.1 | 86.5% | 86.6% | 270T>C;612T>C;1623_1623delTins250 |
| 5 | human | 112574 | SNX18 | sorting nexin 18 | XR_001741987.1 | 52.2% | (many diffs) | |
| 6 | mouse | 170625 | Snx18 | sorting nexin 18 | NM_130796.4 | 89.5% | 95.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1941
- ORF length:
- 1872
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgctgcgc gcccgggcgc tgtacgactt caggtcggag aacccaggag 121 agatctcgct gcgagagcac gaggtgctga gcctgtgcag cgagcaggac atcgagggct 181 ggctcgaggg ggtcaacagc cgcggcgacc gcggcctctt cccggcctcc tatgtgcagg 241 tgatccgcgc ccccgagcct ggcccggcgg gagacggcgg cccgggcgcc ccggcccgct 301 acgccaatgt gccccccggg ggcttcgagc ccctgcccgt cgcgcccccc gcctccttca 361 agccgccgcc tgacgccttc caggcgctgc tgcagccaca gcaggcgccg cctccgagca 421 ccttccagcc gcccggcgcg ggcttcccgt acggcggggg cgccctgcag ccgtcgcctc 481 agcagctcta cggcggctac caggccagcc aaggcagcga tgatgactgg gacgacgagt 541 gggacgacag ctccacggtg gcggacgagc cgggcgctct gggcagcgga gcatacccgg 601 acctcgacgg ctcgtcttcg gcgggtgtgg gcgcagccgg ccgctaccgc ctgtccacgc 661 gctccgacct gtccctgggc tcccgcggcg gctcggtccc cccgcagcac cacccgtcgg 721 ggcccaagag ctcggccacc gtgagccgca acctcaatcg cttctccacc ttcgtcaagt 781 ccggcgggga ggccttcgtg ctgggggagg cgtcaggctt cgtgaaggac ggggacaagc 841 tgtgcgtggt gctggggccc tatggccccg agtggcagga gaacccctac ccgttccagt 901 gcaccatcga cgaccccacc aagcagacca agttcaaggg catgaagagc tacatctcct 961 acaagctggt gcccacgcac acgcaggtgc cggtgcatcg gcgctacaag cacttcgact 1021 ggctgtacgc gcgcctggcg gagaagttcc cggtcatctc cgtgccccac ctgcccgaga 1081 agcaggccac cggccgcttc gaggaggact tcatctctaa gcgcaggaag ggcctgatct 1141 ggtggatgaa ccacatggcc agccacccag tgctggcgca gtgcgacgtc ttccagcact 1201 tcctgacgtg ccccagcagc accgacgaga aagcctggaa gcagggcaag aggaaggccg 1261 agaaggacga gatggtgggc gccaacttct tcctgaccct tagcacgccc cccgccgctg 1321 cccttgacct gcaggaggtg gagagcaaga tcgacggctt caagtgcttc accaagaaga 1381 tggacgacag cgcgctgcag ctcaaccaca cggccaacga gttcgcgcgc aagcaggtga 1441 ccggcttcaa aaaggagtat cagaaggtgg gccagtcctt ccgcggcctc agccaggcct 1501 ttgagctgga ccagcaggcc ttctcggtgg gccTGAACCA GGCTATCGCC TTCACCGGAG 1561 ATGCCTATGA CGCCATTGGC GAGCTCTTCG CGGAGCAGCC CAGGCAGGAC CTGGATCCCG 1621 TCATGGACCT ATTAGCGCTG TATCAGGGGC ATCTGGCTAA CTTCCCGGAC ATCATCCACG 1681 TTCAGAAAGG AGCTCTTACC AAAGTCAAGG AGAGTAGGCG ACACGTGGAG GAAGGGAAGA 1741 TGGAGGTGCA GAAGGCTGAC GGCATTCAGG ATCGCTGTAA CACTATTTCT TTTGCCACTT 1801 TGGCTGAAAT TCACCACTTC CATCAAATTC GAGTGAGAGA CTTTAAATCA CAGATGCAGC 1861 ATTTCTTACA ACAACAAATA ATATTTTTCC AAAAAGTTAC CCAGAAGTTG GAAGAAGCTC 1921 TTCACAAATA TGATAGTGTT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1981 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2041 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGGA ATTGGTGCCG 2101 ATAATAGGAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt