Construct: ORF TRCN0000467809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014066.1_s317c1
- Derived from:
- ccsbBroadEn_15622
- DNA Barcode:
- CCACCCTGTTAGCTGCGGCCAGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZKSCAN1 (7586)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_001346581.2 | 99.9% | 99.8% | 77T>C |
| 2 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_003439.4 | 99.9% | 99.8% | 77T>C |
| 3 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | XM_011516559.3 | 99.9% | 99.8% | 77T>C |
| 4 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_001287054.3 | 93.6% | 93.6% | 0_1ins108 |
| 5 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_001346580.2 | 93.6% | 93.6% | 0_1ins108 |
| 6 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_001287055.2 | 62.1% | 62.1% | 0_1ins639 |
| 7 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_001346579.1 | 51.5% | 43.7% | (many diffs) |
| 8 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NR_144477.2 | 17.4% | (many diffs) | |
| 9 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | XR_927529.2 | 16.6% | (many diffs) | |
| 10 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | XR_002956485.1 | 16.1% | (many diffs) | |
| 11 | mouse | 74570 | Zkscan1 | zinc finger with KRAB and S... | NM_133906.4 | 85.4% | 89.5% | (many diffs) |
| 12 | mouse | 74570 | Zkscan1 | zinc finger with KRAB and S... | NM_029869.1 | 74.2% | 78.3% | (many diffs) |
| 13 | mouse | 74570 | Zkscan1 | zinc finger with KRAB and S... | XM_006504628.3 | 74.2% | 78.3% | (many diffs) |
| 14 | mouse | 74570 | Zkscan1 | zinc finger with KRAB and S... | XM_011240958.2 | 53.1% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1755
- ORF length:
- 1689
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gactgctgaa tcacgggaag ccacgggtct gtccccacag gctgcacagg 121 agaaggatgg tatcgtaata gcgaaggtgg aagaggaaga tgaggaagac cacatgtggg 181 ggcaggattc caccctacag gacacgcctc ctccagaccc agagatattc cgccaacgct 241 tcaggcgctt ctgttaccag aacacttttg ggccccgaga ggctctcagt cggctgaagg 301 aactttgtca tcagtggctg cggccagaaa taaacaccaa ggaacagatc ctggagcttc 361 tggtgctaga gcagtttctt tccatcctgc ccaaggagct ccaggtctgg ctgcaggaat 421 accgccccga tagtggagag gaggccgtga cccttctaga agacttggag cttgatttat 481 caggacaaca ggtcccaggt caagttcatg gacctgagat gctcgcaagg gggatggtgc 541 ctctggatcc agttcaggag tcctcgagct ttgaccttca tcacgaggcc acccagtccc 601 acttcaaaca ttcgtctcgg aaaccccgcc tcttacagtc acgagctctt cctgctgccc 661 acattcctgc accccctcat gagggtagtc ccagagacca ggcgatggca tctgcactat 721 tcacagcgga ttcccaggca atggtgaaga tcgaggacat ggctgtgtcc ctcattctgg 781 aggaatgggg atgtcagaat ctggctcgga ggaatctcag tagggacaac aggcaggaga 841 attatgggag cgcatttccc cagggtggtg aaaacaggaa tgagaacgag gagtcaacct 901 caaaggctga aacctcggaa gattcagcat cacgcgggga gacaacagga agatcccaga 961 aagagtttgg agagaaacgt gaccaggagg gcaaaacagg agaaagacag cagaaaaacc 1021 ctgaggagaa aaccaggaaa gagaaaagag attcagggcc agctatagga aaggacaaaa 1081 aaaccatcac aggagagaga ggtccaaggg agaaggggaa aggattggga agaagcttca 1141 gtctgagctc caacttcacc acccctgaag aagttcccac gggaacaaag tctcacagat 1201 gtgatgaatg tggtaaatgc ttcacgagaa gttcaagcct tatccgccat aaaataatcc 1261 acactggaga aaagccctat gaatgtagtg agtgtgggaa agccttcagt cttaactcca 1321 accttgtcct gcatcagagg atccacacag gagagaaacc TCATGAATGT AACGAGTGTG 1381 GCAAGGCCTT CAGCCACAGT TCCAATCTCA TCCTCCATCA GCGCATCCAC TCTGGAGAGA 1441 AACCTTATGA ATGTAATGAG TGCGGGAAGG CCTTCAGCCA GAGCTCGGAC CTCACCAAGC 1501 ATCAGAGAAT TCACACGGGG GAGAAACCCT ATGAATGTAG TGAATGTGGA AAAGCTTTCA 1561 ACCGAAACTC ATACCTGATT TTGCATCGGA GAATTCACAC TCGAGAAAAG CCCTACAAGT 1621 GCACTAAGTG TGGCAAGGCC TTCACCCGCA GCTCCACCCT CACTCTGCAT CACAGAATCC 1681 ATGCCAGAGA GAGAGCCTCT GAGTACAGCC CAGCCTCCCT TGATGCATTT GGCGCGTTCC 1741 TGAAAAGTTG TGTGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1801 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1861 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCACCCTGTT AGCTGCGGCC 1921 AGAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt