Construct: ORF TRCN0000467815
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005158.1_s317c1
- Derived from:
- ccsbBroadEn_12821
- DNA Barcode:
- AGGGAGCTCCCAACTCAGTTTGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHYHIPL (84457)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467815
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84457 | PHYHIPL | phytanoyl-CoA 2-hydroxylase... | XM_011540275.3 | 99.8% | 99.6% | 886G>C |
| 2 | human | 84457 | PHYHIPL | phytanoyl-CoA 2-hydroxylase... | XM_011540276.3 | 99.8% | 99.6% | 886G>C |
| 3 | human | 84457 | PHYHIPL | phytanoyl-CoA 2-hydroxylase... | XM_017016782.1 | 99.8% | 99.6% | 886G>C |
| 4 | human | 84457 | PHYHIPL | phytanoyl-CoA 2-hydroxylase... | XM_017016783.2 | 99.8% | 99.6% | 886G>C |
| 5 | human | 84457 | PHYHIPL | phytanoyl-CoA 2-hydroxylase... | NM_001143774.1 | 94.1% | 94% | 1_60del;946G>C |
| 6 | human | 84457 | PHYHIPL | phytanoyl-CoA 2-hydroxylase... | NM_032439.4 | 87.6% | 87.5% | 1_138del;1024G>C |
| 7 | mouse | 70911 | Phyhipl | phytanoyl-CoA hydroxylase i... | NM_001162846.1 | 91.7% | 97.8% | (many diffs) |
| 8 | mouse | 70911 | Phyhipl | phytanoyl-CoA hydroxylase i... | XM_006514112.3 | 84.5% | 90.2% | (many diffs) |
| 9 | mouse | 70911 | Phyhipl | phytanoyl-CoA hydroxylase i... | NM_178621.4 | 80.7% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1056
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaacttcct gtaccacata acatcaaaat aagcaatata acgtgtgact 121 cattcaagat ttcatgggaa atggattcaa aatcaaagga tcgcattaca cactatttta 181 ttgacctcaa caagaaagag aacaagaact ccaataaatt taaacacaag gatgttccca 241 caaaattggt ggcaaaagct gttcccttgc ctatgactgt ccgtggacac tggtttttaa 301 gcccaagaac tgaatataca gtagcagtgc agactgcctc aaaacaagtt gatggtgatt 361 atgttgtgtc tgaatggagt gaaattatag aattctgcac cgcagactat tcaaaagttc 421 atctaacaca attgttggag aaggctgaag tgattgcagg acgcatgctt aagttttctg 481 ttttttatcg taatcagcac aaagaatatt ttgactatgt tcgagaacat catgggaatg 541 ctatgcagcc atctgtcaag gataacagtg gtagccatgg ctctcctatc agtggaaaat 601 tagaaggcat cttcttcagc tgcagcactg aattcaatac tggaaagcca ccccaggatt 661 caccttatgg aagatacagg tttgagattg ccgcagaaaa actttttaac ccCAATACTA 721 ACTTATACTT TGGGGACTTC TACTGTATGT ACACTGCTTA TCATTATGTG ATTCTTGTTA 781 TTGCTCCTGT GGGATCACCA GGAGATGAAT TTTGTAAGCA GCGCCTTCCT CAACTAAATT 841 CTAAGGATAA TAAATTTTTG ACCTGTACAG AAGAAGATGG GGTGCTGGTT TACCACCATG 901 CCCAGGATGT CATTTTAGAA GTCATTTACA CTGACCCTGT GGATCTTTCT CTGGGCACCG 961 TGGCAGAAAT CACTGGTCAT CAGCTCATGA GTTTGTCTAC TGCAAATGCA AAGAAAGATC 1021 CCAGCTGCAA AACCTGTAAT ATCAGTGTTG GACGTTACCC AACTTTCTTG TACAAAGTGG 1081 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1141 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1201 AAGGGAGCTC CCAACTCAGT TTGGAACGCG TTAAGTCgac aatcaacctc tggattacaa 1261 aatttgtgaa agatt