Construct: ORF TRCN0000467830
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001736.1_s317c1
- Derived from:
- ccsbBroadEn_05270
- DNA Barcode:
- CACTTATTGTTCCCGTGCCTCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATXN7L1 (222255)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467830
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | NM_152749.3 | 100% | 100% | |
| 2 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XM_011515954.3 | 100% | 100% | |
| 3 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XM_011515950.2 | 29.5% | 26.2% | (many diffs) |
| 4 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XM_006715903.4 | 16.3% | 14.4% | (many diffs) |
| 5 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | NM_020725.1 | 16.2% | 14.4% | (many diffs) |
| 6 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XM_011515940.2 | 16.2% | 14.4% | (many diffs) |
| 7 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XM_005250220.4 | 16.1% | 14.3% | (many diffs) |
| 8 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XM_011515939.2 | 16% | 14.2% | (many diffs) |
| 9 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XM_006715902.4 | 16% | 14.2% | (many diffs) |
| 10 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XM_011515937.3 | 15.9% | 14.2% | (many diffs) |
| 11 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XM_011515935.2 | 14.4% | 12.8% | (many diffs) |
| 12 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XM_006715901.3 | 14.4% | 12.8% | (many diffs) |
| 13 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XM_006715900.3 | 14.3% | 12.7% | (many diffs) |
| 14 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XR_001744596.2 | 12.4% | (many diffs) | |
| 15 | human | 222255 | ATXN7L1 | ataxin 7 like 1 | XR_927411.2 | 11.6% | (many diffs) | |
| 16 | mouse | 380753 | Atxn7l1 | ataxin 7-like 1 | NM_028139.5 | 94.2% | 91.7% | (many diffs) |
| 17 | mouse | 380753 | Atxn7l1 | ataxin 7-like 1 | XM_006515130.3 | 15.8% | 14.2% | (many diffs) |
| 18 | mouse | 380753 | Atxn7l1 | ataxin 7-like 1 | XM_006515129.3 | 15.7% | 14.1% | (many diffs) |
| 19 | mouse | 380753 | Atxn7l1 | ataxin 7-like 1 | XM_006515128.3 | 15.6% | 14% | (many diffs) |
| 20 | mouse | 380753 | Atxn7l1 | ataxin 7-like 1 | XM_006515125.3 | 14.1% | 12.6% | (many diffs) |
| 21 | mouse | 380753 | Atxn7l1 | ataxin 7-like 1 | XM_006515124.3 | 14.1% | 12.6% | (many diffs) |
| 22 | mouse | 380753 | Atxn7l1 | ataxin 7-like 1 | XM_006515123.3 | 13.9% | 12.5% | (many diffs) |
| 23 | mouse | 380753 | Atxn7l1 | ataxin 7-like 1 | XM_006515126.3 | 11.6% | 10.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 504
- ORF length:
- 438
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gtcggagcgt tctcgaatcc cgtgtctctc ggctgctgct gccgaaggaa 121 cagggaaaaa gcaacaagaa ggaagagcaa tggcgacact ggatcgcaaa gtgcccagtc 181 cggaggcgtt tctgggcaaa ccctggtccT CCTGGATCGA CGCCGCCAAA TTACACTGCT 241 CCGACAATGT AGATTTAGAA GAGGCTGGAA AAGAGGGTGG AAAAAGCAGG GAGGTTATGA 301 GGCTTAATAA AGAAGATATG CACTTATTTG GCCATTACCC AGCACATGAC GACTTCTATC 361 TCGTAGTGTG CAGTGCCTGT AACCAGGTCG TCAAGCCACA GGTTTTCCAG TCGCACTGCG 421 GGAGAAAGCA AGACAACAGG AGAAATGAAG GCATCTCCAG GAGTGGACCA GAGAGCAGCC 481 AAGCCATAGA GAAGCATCAG GTGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 541 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 601 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC ACTTATTGTT 661 CCCGTGCCTC GCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 721 att