Transcript: Human NM_020725.1

Homo sapiens ataxin 7 like 1 (ATXN7L1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-04
Taxon:
Homo sapiens (human)
Gene:
ATXN7L1 (222255)
Length:
5691
CDS:
28..2613

Additional Resources:

NCBI RefSeq record:
NM_020725.1
NBCI Gene record:
ATXN7L1 (222255)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020725.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000105007 TCCGACAATGTAGATTTAGAA pLKO.1 202 CDS 100% 5.625 7.875 N Atxn7l1 n/a
2 TRCN0000136260 GCAACAAGAAGGAAGAGCAAT pLKO.1 93 CDS 100% 4.950 6.930 N ATXN7L1 n/a
3 TRCN0000105008 CAAATTACACTGCTCCGACAA pLKO.1 189 CDS 100% 4.050 5.670 N Atxn7l1 n/a
4 TRCN0000135765 CAAATTACACTGCTCCGACAA pLKO.1 189 CDS 100% 4.050 5.670 N ATXN7L1 n/a
5 TRCN0000432531 TGACGACTTCTATCTCGTAGT pLKO_005 309 CDS 100% 4.050 5.670 N ATXN7L1 n/a
6 TRCN0000433578 TATGCACTTATTTGGCCATTA pLKO_005 279 CDS 100% 10.800 8.640 N ATXN7L1 n/a
7 TRCN0000348933 GGAGGTTATGAGGCTTAATAA pLKO_005 252 CDS 100% 15.000 10.500 N Atxn7l1 n/a
8 TRCN0000105006 AGGGAGGTTATGAGGCTTAAT pLKO.1 250 CDS 100% 13.200 9.240 N Atxn7l1 n/a
9 TRCN0000217153 CTTCACCAGAGAAGATCTTAA pLKO.1 782 CDS 100% 13.200 9.240 N Atxn7l1 n/a
10 TRCN0000348871 TTCACCAGAGAAGATCTTAAA pLKO_005 783 CDS 100% 13.200 9.240 N Atxn7l1 n/a
11 TRCN0000138057 CAGGGAGGTTATGAGGCTTAA pLKO.1 249 CDS 100% 10.800 7.560 N ATXN7L1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020725.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13436 pDONR223 100% 40.7% 39.5% None (many diffs) n/a
2 ccsbBroad304_13436 pLX_304 0% 40.7% 39.5% V5 (many diffs) n/a
3 TRCN0000474251 TGACTGAAATGCCAATCCTTACAA pLX_317 30.6% 40.7% 39.5% V5 (many diffs) n/a
4 ccsbBroadEn_05270 pDONR223 100% 16.2% 14.4% None (many diffs) n/a
5 ccsbBroad304_05270 pLX_304 0% 16.2% 14.4% V5 (many diffs) n/a
6 TRCN0000467830 CACTTATTGTTCCCGTGCCTCGCA pLX_317 66.5% 16.2% 14.4% V5 (many diffs) n/a
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