Construct: ORF TRCN0000468030
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017337.1_s317c1
- Derived from:
- ccsbBroadEn_08500
- DNA Barcode:
- CATCTCCCAAGAGGATTAGTACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECHDC2 (55268)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468030
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | NM_001198961.2 | 99.7% | 99.6% | 355A>G;714C>G |
| 2 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_024448153.1 | 94.2% | 94.1% | 355A>G;714C>G;751_752ins48 |
| 3 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | NM_018281.4 | 89.1% | 89% | 355A>G;363_364ins93;621C>G |
| 4 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_011541709.2 | 83.8% | 77.1% | (many diffs) |
| 5 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_024448160.1 | 83.6% | 83.5% | (many diffs) |
| 6 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | NM_001198962.1 | 83.4% | 83.2% | 355A>G;363_364ins93;607_608ins51 |
| 7 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_024448161.1 | 82.6% | 82.5% | 355A>G;363_364ins150;564C>G |
| 8 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_024448152.1 | 74.4% | 67.7% | (many diffs) |
| 9 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | NM_001319958.2 | 73.4% | 73.2% | (many diffs) |
| 10 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_024448163.1 | 73.4% | 73.2% | (many diffs) |
| 11 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_024448164.1 | 73.4% | 73.2% | (many diffs) |
| 12 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_011541713.3 | 69.8% | 63.2% | (many diffs) |
| 13 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_011541715.3 | 69.8% | 63.2% | (many diffs) |
| 14 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_011541719.2 | 69.8% | 63.2% | (many diffs) |
| 15 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_011541720.3 | 69.8% | 63.2% | (many diffs) |
| 16 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_011541722.2 | 69.8% | 63.2% | (many diffs) |
| 17 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_011541723.2 | 69.8% | 63.2% | (many diffs) |
| 18 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_017001638.1 | 69.8% | 63.2% | (many diffs) |
| 19 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_017001639.1 | 69.8% | 63.2% | (many diffs) |
| 20 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_017001640.1 | 69.8% | 63.2% | (many diffs) |
| 21 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_024448157.1 | 69.8% | 63.2% | (many diffs) |
| 22 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_024448158.1 | 69.8% | 63.2% | (many diffs) |
| 23 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_024448159.1 | 68.6% | 62% | (many diffs) |
| 24 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_011541726.3 | 60.4% | 53.9% | (many diffs) |
| 25 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XR_002957011.1 | 59.7% | (many diffs) | |
| 26 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XM_011541727.3 | 54.6% | 48.1% | (many diffs) |
| 27 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XR_002957012.1 | 49.3% | (many diffs) | |
| 28 | human | 55268 | ECHDC2 | enoyl-CoA hydratase domain ... | XR_002957013.1 | 46.4% | (many diffs) | |
| 29 | mouse | 52430 | Echdc2 | enoyl Coenzyme A hydratase ... | NM_026728.4 | 83.2% | 85.1% | (many diffs) |
| 30 | mouse | 52430 | Echdc2 | enoyl Coenzyme A hydratase ... | NM_001254754.1 | 55.6% | 58.9% | (many diffs) |
| 31 | mouse | 52430 | Echdc2 | enoyl Coenzyme A hydratase ... | XM_011240573.1 | 55.6% | 58.9% | (many diffs) |
| 32 | mouse | 52430 | Echdc2 | enoyl Coenzyme A hydratase ... | XM_017320314.1 | 55.6% | 58.9% | (many diffs) |
| 33 | mouse | 52430 | Echdc2 | enoyl Coenzyme A hydratase ... | XM_017320315.1 | 47.1% | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcgcgttctg tgcctcctgc gcccctggag gccccttcgg gcccgcggct 121 gcgcttccga cggggcggcc gggggctcag agatccaagt gcgcgccctg gcgggtccgg 181 accaagggat cactgagatt ctgatgaaca gaccttctgc ccgcaatgcc ttggggaatg 241 tcttcgtcag tgagctgctg gaaactctgg cccagctgcg ggaggaccgg caagtgcgtg 301 tcctgctctt cagaagtgga gtgaagggcg tgttctgtgc aggtgcagac ctgaaggagc 361 gggaacagat gagtgaagca gaggtggggg tgtttgtcca gcgactccgg ggcctgatgg 421 atgacatcgc agccttccct gcacccacca ttgcggctat ggatgggttt gccttgggcg 481 gaggcctaga gcttgccctg gcctgtgacc TCCGAGTGGC AGCTTCCTCG GCAGTCATGG 541 GACTGATTGA GACCACGCGA GGGCTCCTCC CGGGGGCAGG AGGGACTCAG AGGCTGCCCC 601 GTTGTCTGGG GGTGGCCCTG GCGAAGGAGC TCATCTTCAC GGGCCGACGA CTGAGTGGAA 661 CTGAGGCCCA CGTACTGGGG CTGGTGAATC ACGCTGTGGC CCAGAACGAG GAGGGGGACG 721 CCGCCTACCA GCGGGCACGA GCACTGGCCC AGGAGATCCT GCCCCAGGCC CCCATTGCGG 781 TGCGGCTGGG CAAAGTAGCC ATTGACCGAG GAACGGAGGT GGACATTGCA TCTGGGATGG 841 CCATTGAAGG GATGTGCTAT GCCCAGAATA TTCCAACCCG GGACCGGCTA GAGGGCATGG 901 CAGCCTTCAG GGAGAAGCGG ACTCCCAAAT TTGTTGGCAA ATGCCCAACT TTCTTGTACA 961 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1021 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1081 AGGACGACAT CTCCCAAGAG GATTAGTACT AACGCGTTAA GTCgacaatc aacctctgga 1141 ttacaaaatt tgtgaaagat t