Construct: ORF TRCN0000468120
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017780.1_s317c1
- Derived from:
- ccsbBroadEn_07333
- DNA Barcode:
- GCAGCACCCCACCATAATCTTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H3C7 (8968)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468120
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8968 | H3C7 | H3 clustered histone 7 | NM_021018.2 | 99.5% | 100% | 6A>T;9C>T |
| 2 | human | 8351 | H3C4 | H3 clustered histone 4 | NM_003530.4 | 88.2% | 100% | (many diffs) |
| 3 | human | 8357 | H3C10 | H3 clustered histone 10 | NM_003536.2 | 87.5% | 100% | (many diffs) |
| 4 | human | 8354 | H3C11 | H3 clustered histone 11 | NM_003533.2 | 86.7% | 100% | (many diffs) |
| 5 | human | 8355 | H3C8 | H3 clustered histone 8 | NM_003534.2 | 86% | 100% | (many diffs) |
| 6 | human | 333932 | H3C15 | H3 clustered histone 15 | NM_001005464.2 | 86% | 99.2% | (many diffs) |
| 7 | human | 126961 | H3C14 | H3 clustered histone 14 | NM_021059.2 | 86% | 99.2% | (many diffs) |
| 8 | human | 8350 | H3C1 | H3 clustered histone 1 | NM_003529.2 | 85.7% | 100% | (many diffs) |
| 9 | human | 8356 | H3C12 | H3 clustered histone 12 | NM_003535.2 | 84.8% | 100% | (many diffs) |
| 10 | human | 653604 | H3C13 | H3 clustered histone 13 | NM_001123375.2 | 84.8% | 99.2% | (many diffs) |
| 11 | human | 8353 | H3C6 | H3 clustered histone 6 | NM_003532.2 | 84.5% | 100% | (many diffs) |
| 12 | human | 8358 | H3C2 | H3 clustered histone 2 | NM_003537.3 | 84.5% | 100% | (many diffs) |
| 13 | human | 8290 | H3-4 | H3.4 histone | NM_003493.2 | 84.5% | 97% | (many diffs) |
| 14 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001355409.3 | 84.3% | 97% | (many diffs) |
| 15 | human | 440686 | H3-2 | H3.2 histone (putative) | NM_001372105.1 | 84.3% | 97% | (many diffs) |
| 16 | human | 8352 | H3C3 | H3 clustered histone 3 | NM_003531.2 | 83.5% | 100% | (many diffs) |
| 17 | human | 3020 | H3-3A | H3.3 histone A | NM_002107.6 | 78.8% | 96.3% | (many diffs) |
| 18 | human | 440093 | H3-5 | H3.5 histone | NM_001013699.3 | 78.2% | 93.3% | (many diffs) |
| 19 | human | 3021 | H3-3B | H3.3 histone B | NM_005324.5 | 78% | 96.3% | (many diffs) |
| 20 | mouse | 319148 | Hist1h3c | histone cluster 1, H3c | NM_175653.2 | 87.5% | 99.2% | (many diffs) |
| 21 | mouse | 319153 | Hist1h3i | histone cluster 1, H3i | NM_178207.2 | 87.2% | 100% | (many diffs) |
| 22 | mouse | 319152 | Hist1h3h | histone cluster 1, H3h | NM_178206.2 | 87% | 100% | (many diffs) |
| 23 | mouse | 319150 | Hist1h3b | histone cluster 1, H3b | NM_178203.2 | 87% | 99.2% | (many diffs) |
| 24 | mouse | 97908 | Hist1h3g | histone cluster 1, H3g | NM_145073.2 | 86.7% | 100% | (many diffs) |
| 25 | mouse | 319149 | Hist1h3d | histone cluster 1, H3d | NM_178204.2 | 86.7% | 99.2% | (many diffs) |
| 26 | mouse | 360198 | Hist1h3a | histone cluster 1, H3a | NM_013550.5 | 86.2% | 100% | (many diffs) |
| 27 | mouse | 260423 | Hist1h3f | histone cluster 1, H3f | NM_013548.4 | 86.2% | 99.2% | (many diffs) |
| 28 | mouse | 319151 | Hist1h3e | histone cluster 1, H3e | NM_178205.2 | 86.2% | 99.2% | (many diffs) |
| 29 | mouse | 97114 | Hist2h3c2 | histone cluster 2, H3c2 | NM_054045.4 | 85.5% | 99.2% | (many diffs) |
| 30 | mouse | 319154 | Hist2h3b | histone cluster 2, H3b | NM_178215.2 | 85.5% | 99.2% | (many diffs) |
| 31 | mouse | 15077 | Hist2h3c1 | histone cluster 2, H3c1 | NM_178216.3 | 85.5% | 99.2% | (many diffs) |
| 32 | mouse | 382523 | Gm12260 | predicted gene 12260 | NM_001318003.2 | 84.5% | 97.7% | (many diffs) |
| 33 | mouse | 15081 | H3f3b | H3 histone, family 3B | NM_008211.3 | 78% | 96.3% | (many diffs) |
| 34 | mouse | 15081 | H3f3b | H3 histone, family 3B | XM_006532248.2 | 78% | 96.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 477
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcTCGTACG AAGCAAACAG CTCGTAAGTC CACTGGCGGC AAAGCCCCGC 121 GCAAGCAGCT GGCCACTAAG GCGGCTCGCA AAAGCGCGCC AGCCACCGGT GGCGTGAAGA 181 AGCCCCACCG CTACAGGCCT GGTACTGTCG CCCTCCGTGA AATCCGCCGC TATCAGAAAT 241 CGACTGAGCT ACTGATTCGC AAGCTACCAT TCCAGCGTCT GGTACGTGAG ATCGCGCAGG 301 ACTTCAAGAC CGACCTGCGC TTCCAGAGCT CGGCTGTGAT GGCGCTGCAG GAGGCCTGCG 361 AGGCTTACCT GGTGGGGCTC TTTGAGGACA CCAACCTGTG TGCTATCCAC GCCAAGCGAG 421 TGACTATCAT GCCCAAGGAC ATCCAGCTCG CTCGCCGCAT TCGCGGAGAG AGGGCATTGC 481 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 541 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 601 ATATATCTTG TGGAAAGGAC GAGCAGCACC CCACCATAAT CTTTGAACGC GTTAAGTCga 661 caatcaacct ctggattaca aaatttgtga aagatt