Construct: ORF TRCN0000468126

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001398.1_s317c1
Derived from:
ccsbBroadEn_07813
DNA Barcode:
TTTACTGCCCTAACGTCACTTTAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CARD8 (22900)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468126
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22900 CARD8 caspase recruitment domain ... NM_001184902.2 98.5% 98.4% 246T>C;758T>C;1162_1176del
2 human 22900 CARD8 caspase recruitment domain ... NM_001184903.1 98.5% 98.4% 246T>C;758T>C;1162_1176del
3 human 22900 CARD8 caspase recruitment domain ... XM_006723106.4 98.5% 98.4% 246T>C;758T>C;1162_1176del
4 human 22900 CARD8 caspase recruitment domain ... XM_011526650.2 98.5% 98.4% 246T>C;758T>C;1162_1176del
5 human 22900 CARD8 caspase recruitment domain ... XM_006723107.3 90.1% 89.1% (many diffs)
6 human 22900 CARD8 caspase recruitment domain ... NM_001351788.2 85.7% 85.7% (many diffs)
7 human 22900 CARD8 caspase recruitment domain ... NM_001351789.2 85.7% 85.7% (many diffs)
8 human 22900 CARD8 caspase recruitment domain ... XM_017026492.2 85.7% 85.7% (many diffs)
9 human 22900 CARD8 caspase recruitment domain ... XM_017026485.1 83.2% 83.1% 246T>C;758T>C;1162_1392del
10 human 22900 CARD8 caspase recruitment domain ... NM_001351790.2 77.8% 68% (many diffs)
11 human 22900 CARD8 caspase recruitment domain ... NM_001184900.3 71.9% 71.8% 246T>C;758T>C;1162_1611del
12 human 22900 CARD8 caspase recruitment domain ... NM_001351782.2 71.9% 71.8% 246T>C;758T>C;1162_1611del
13 human 22900 CARD8 caspase recruitment domain ... XM_006723091.4 71.9% 71.8% 246T>C;758T>C;1162_1611del
14 human 22900 CARD8 caspase recruitment domain ... XM_006723092.4 71.9% 71.8% 246T>C;758T>C;1162_1611del
15 human 22900 CARD8 caspase recruitment domain ... XM_006723093.4 71.9% 71.8% 246T>C;758T>C;1162_1611del
16 human 22900 CARD8 caspase recruitment domain ... XM_011526641.3 71.9% 71.8% 246T>C;758T>C;1162_1611del
17 human 22900 CARD8 caspase recruitment domain ... XM_011526643.3 71.9% 71.8% 246T>C;758T>C;1162_1611del
18 human 22900 CARD8 caspase recruitment domain ... XM_011526644.3 71.9% 71.8% 246T>C;758T>C;1162_1611del
19 human 22900 CARD8 caspase recruitment domain ... NM_001351791.2 71.8% 66.8% (many diffs)
20 human 22900 CARD8 caspase recruitment domain ... NM_001351792.2 71.8% 66.8% (many diffs)
21 human 22900 CARD8 caspase recruitment domain ... XM_006723094.4 71.7% 71.6% (many diffs)
22 human 22900 CARD8 caspase recruitment domain ... XM_017026482.2 71.7% 71.6% (many diffs)
23 human 22900 CARD8 caspase recruitment domain ... XM_006723109.3 71.5% 66.5% (many diffs)
24 human 22900 CARD8 caspase recruitment domain ... XM_017026498.1 71.5% 66.5% (many diffs)
25 human 22900 CARD8 caspase recruitment domain ... XM_017026499.1 69.3% 68.8% (many diffs)
26 human 22900 CARD8 caspase recruitment domain ... NM_001184901.1 62.6% 62.5% (many diffs)
27 human 22900 CARD8 caspase recruitment domain ... NM_001351783.2 62.6% 62.5% (many diffs)
28 human 22900 CARD8 caspase recruitment domain ... NM_014959.5 62.6% 62.5% (many diffs)
29 human 22900 CARD8 caspase recruitment domain ... XM_006723096.4 62.6% 62.5% (many diffs)
30 human 22900 CARD8 caspase recruitment domain ... XM_006723097.4 62.6% 62.5% (many diffs)
31 human 22900 CARD8 caspase recruitment domain ... XM_017026483.2 62.6% 62.5% (many diffs)
32 human 22900 CARD8 caspase recruitment domain ... XM_017026484.2 62.4% 62.3% (many diffs)
33 human 22900 CARD8 caspase recruitment domain ... NM_001351787.1 60.7% 56.4% (many diffs)
34 human 22900 CARD8 caspase recruitment domain ... XM_011526646.3 58% 50% (many diffs)
35 human 22900 CARD8 caspase recruitment domain ... XM_011526647.3 58% 50% (many diffs)
36 human 22900 CARD8 caspase recruitment domain ... XM_011526648.3 57.8% 49.8% (many diffs)
37 human 22900 CARD8 caspase recruitment domain ... XM_006723098.3 56.8% 49.8% (many diffs)
38 human 22900 CARD8 caspase recruitment domain ... XM_024451421.1 56.8% 49.8% (many diffs)
39 human 22900 CARD8 caspase recruitment domain ... XM_024451422.1 56.6% 49.6% (many diffs)
40 human 22900 CARD8 caspase recruitment domain ... NM_001351784.2 52.4% 48.7% (many diffs)
41 human 22900 CARD8 caspase recruitment domain ... XM_006723102.4 52.4% 48.7% (many diffs)
42 human 22900 CARD8 caspase recruitment domain ... XM_024451423.1 52.4% 48.7% (many diffs)
43 human 22900 CARD8 caspase recruitment domain ... NM_001365950.1 52.2% 48.6% (many diffs)
44 human 22900 CARD8 caspase recruitment domain ... XM_006723104.3 52.2% 48.6% (many diffs)
45 human 22900 CARD8 caspase recruitment domain ... NM_001351786.2 50.8% 50.2% (many diffs)
46 human 22900 CARD8 caspase recruitment domain ... XM_017026487.2 50.8% 50.2% (many diffs)
47 human 22900 CARD8 caspase recruitment domain ... XM_017026488.2 50.8% 50.2% (many diffs)
48 human 22900 CARD8 caspase recruitment domain ... XM_024451424.1 50.8% 50.4% (many diffs)
49 human 22900 CARD8 caspase recruitment domain ... XM_024451425.1 50.8% 50.4% (many diffs)
50 human 22900 CARD8 caspase recruitment domain ... XR_001753636.1 41.1% (many diffs)
51 human 22900 CARD8 caspase recruitment domain ... XR_001753637.2 37.9% (many diffs)
52 human 22900 CARD8 caspase recruitment domain ... NR_147707.1 34% (many diffs)
53 human 22900 CARD8 caspase recruitment domain ... XR_430193.3 21.9% (many diffs)
54 human 22900 CARD8 caspase recruitment domain ... NR_033679.1 21.1% (many diffs)
55 human 22900 CARD8 caspase recruitment domain ... NR_033680.1 18.3% (many diffs)
56 human 22900 CARD8 caspase recruitment domain ... NM_001184904.1 18% 18% 210_211ins951
57 human 22900 CARD8 caspase recruitment domain ... XM_006723110.3 18% 18% 210_211ins951
58 human 22900 CARD8 caspase recruitment domain ... NR_033678.1 17.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1227
ORF length:
1161
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga aaaaaaggag tgtccagaaa agagtagcag cagtgaggaa gagctgccga
121gacgggacag tggatccagt aggaacatag atgcatccaa actcattaga ctacaaggat
181cacggaaact gttggttgac aatagcatac gggaactgca atacacaaaa actggaattt
241tttttcaggc tgaggcctgt gtgacaaatg atacggtata cagggagcta ccctgtgttt
301ctgagaccct ctgtgacatc tcacattttt tccaagaaga tgatgagaca gaggcagagc
361cattattgtt ccgtgctgtt cctgagtgtc aactatctgg gggggacatt cccagtgtat
421cagaagagca ggaatcttca gagggacaag attcaggaga catttgctca gaagagaatc
481aaatagtttc ctcttatgct tctaaagtct gttttgagat cgaagaagat tataaaaatc
541gtcagtttct ggggcctgaa ggaaatgtgg atgttgagtt gattgataag agcacaaaca
601gatacagcgt ttggttcccc actgctggct ggtatctgtg gtcagccaca ggcctcggct
661tcctggtaag ggatgaggtc acagtgacga ttgcgtttgg ttcctggagt cagcacctgg
721ccctggacct gcagcaccat gaacagtggc tggtgggcgg ccccttgttt gatgtcactg
781cagagccaga ggaggctgtc gccgaaatcc acctccccca ctccatctcc ctccaagcag
841gtgaggtgga cgtctcctgg tttctcgttg cccattttaa gaatgaaggG ATGGTCCTGG
901AGCATCCAGC CCGGGTGGAG CCTTTCTATG CTGTCCTGGA AAGCCCCAGC TTCTCTCTGA
961TGGGCATCCT GCTGCGGATC GCCAGTGGGA CTCGCCTCTC CATCCCCATC ACTTCCAACA
1021CATTGATCTA TTATCACCCC CACCCCGAAG ATATTAAGTT CCACTTGTAC CTTGTCCCCA
1081GCGACGCCTT GCTAACAAAG GCGATAGATG ATGAGGAAGA TCGCTTCCAT GGTGTGCGCC
1141TGCAGACTTC GCCCCCAATG GAACCCCTGA ACTTTGGTTC CAGTTATATT GTGTCTAATT
1201CTGCTAACCT GAAAGTAATG CCCAAGTGGA TCTCCAGCTT GTACCCAACT TTCTTGTACA
1261AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1321AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1381AGGACGATTT ACTGCCCTAA CGTCACTTTA CACGCGTTAA GTCgacaatc aacctctgga
1441ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)