Construct: ORF TRCN0000468136
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002042.1_s317c1
- Derived from:
- ccsbBroadEn_14372
- DNA Barcode:
- CCCAGTTGCAGAGTGTCGAATTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PODN (127435)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468136
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127435 | PODN | podocan | XM_011540677.2 | 99.6% | 99.2% | (many diffs) |
| 2 | human | 127435 | PODN | podocan | NM_001199080.4 | 65.3% | 65% | (many diffs) |
| 3 | human | 127435 | PODN | podocan | NM_001199081.3 | 65.3% | 65% | (many diffs) |
| 4 | human | 127435 | PODN | podocan | NM_153703.5 | 65.3% | 65% | (many diffs) |
| 5 | human | 127435 | PODN | podocan | XM_005270457.3 | 65.3% | 65% | (many diffs) |
| 6 | human | 127435 | PODN | podocan | XM_017000294.2 | 61.4% | 61.1% | (many diffs) |
| 7 | human | 127435 | PODN | podocan | XR_001736973.2 | 39.2% | (many diffs) | |
| 8 | mouse | 242608 | Podn | podocan | XM_011240532.1 | 86.7% | 92.2% | (many diffs) |
| 9 | mouse | 242608 | Podn | podocan | XM_011240534.1 | 86.7% | 92.2% | (many diffs) |
| 10 | mouse | 242608 | Podn | podocan | XM_030253568.1 | 86.7% | 92.2% | (many diffs) |
| 11 | mouse | 242608 | Podn | podocan | NM_001285956.1 | 56.8% | 60.4% | (many diffs) |
| 12 | mouse | 242608 | Podn | podocan | NM_001285958.1 | 56.8% | 60.4% | (many diffs) |
| 13 | mouse | 242608 | Podn | podocan | NM_172874.3 | 56.8% | 60.4% | (many diffs) |
| 14 | mouse | 242608 | Podn | podocan | XM_006503092.4 | 56.8% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1272
- ORF length:
- 1206
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cagcggctac agcaacgtcg aggtcctcat cctgtccagc aacttcctgc 121 gccacgtgcc caagcacctg ccgcctgccc tgtacaagct gcacctcaag aacaacaagc 181 tggagaagat ccccccgggg gccttcagcg agctgagcag cctgcgcgag ctatacctgc 241 agaacaacta cctgactgac gagggcctgg acaacgagac cttctggaag ctctccagcc 301 tggagtacct ggatctgtcc agcaacaacc tgtctcgggt cccagctggg ctgccgcgca 361 gcctggtgct gctgcacttg gagaagaacg ccatccggag cgtggacgcg aatgtgctga 421 cccccatccg cagcctggag tacctgctgc tgcacagcaa ccagctgcgg gagcagggca 481 tccacccact ggccttccag ggcctcaagc ggttgcacac ggtgcacctg tacaacaacg 541 cgctggagcg cgtgcccagt ggcctgcctc gccgcgtgcg caccctcatg atcctgcaca 601 accagatcac aggcattggc cgcgaagact ttgccaccac ctacttcctg gaggagctca 661 acctcagcta caaccgcatc accagcccac aggtgcaccg cgacgccttc cgcaagctgc 721 gcctgctgcg ctcgctggac ctgtcgggca accggctgca cacgctgcca cctgggctgc 781 ctcgaaatgt ccatgtgctg aaggtcaagc gcaatgagct ggctgccttg gcacgagggg 841 cgctggcggg catggcTCAG CTGCGTGAGC TGTACCTCAC CAGCAACCGA CTGCGCAGCC 901 GAGCCCTGGG CCCCCGTGCC TGGGTGGACC TCGCCCATCT GCAGCTGCTG GACATCGCCG 961 GGAATCAGCT CACAGAGATC CCCGAGGGGC TCCCCGAGTC ACTTGAGTAC CTGTACCTGC 1021 AGAACAACAA GATTAGTGCG GTGCCCGCCA ATGCCTTCGA CTCCACGCCC AACCTCAAGG 1081 GGATCTTTCT CAGGTTTAAC AAGCTGGCTG TGGGCTCCGT GGTGGACAGT GCCTTCCGGA 1141 GGCTGAAGCA CCTGCAGGTC TTGGACATTG AAGGCAACTT AGAGTTTGGT GACATTTCCA 1201 AGGACCGTGG CCGCTTGGGG AAGGAAAAGG AGGAGGAGGA AGAGGAGGAG GAGGAGGAAG 1261 AGGAAACAAG ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1321 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1381 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCC AGTTGCAGAG TGTCGAATTC 1441 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t