Transcript: Human XM_017000294.2

PREDICTED: Homo sapiens podocan (PODN), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PODN (127435)
Length:
3648
CDS:
673..2631

Additional Resources:

NCBI RefSeq record:
XM_017000294.2
NBCI Gene record:
PODN (127435)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017000294.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000152038 CAACTTAGAGTTTGGTGACAT pLKO.1 2532 CDS 100% 4.950 6.930 N PODN n/a
2 TRCN0000157135 GCAGAACAACTACCTGACTGA pLKO.1 1596 CDS 100% 2.640 3.696 N PODN n/a
3 TRCN0000153180 GCATCTGACCAACCTCAATTA pLKO.1 1218 CDS 100% 13.200 10.560 N PODN n/a
4 TRCN0000157711 CGAGTCACTTGAGTACCTGTA pLKO.1 2352 CDS 100% 4.050 3.240 N PODN n/a
5 TRCN0000154039 CCTGTACTTGGCCAATAACAA pLKO.1 1239 CDS 100% 5.625 3.938 N PODN n/a
6 TRCN0000157674 GAGCATCTGACCAACCTCAAT pLKO.1 1216 CDS 100% 4.950 3.465 N PODN n/a
7 TRCN0000158001 CAACCACCTATCTCTGCAGAA pLKO.1 1083 CDS 100% 4.050 2.835 N PODN n/a
8 TRCN0000156400 CTGGAGAAGACACAAGGGTAT pLKO.1 3158 3UTR 100% 4.050 2.835 N PODN n/a
9 TRCN0000157932 CCTCAAGAACAACAAGCTGGA pLKO.1 1521 CDS 100% 2.160 1.512 N PODN n/a
10 TRCN0000158324 CATGCACAAGTCATGTGCGAA pLKO.1 3041 3UTR 100% 0.264 0.185 N PODN n/a
11 TRCN0000153731 CCAACCTCAATTACCTGTACT pLKO.1 1226 CDS 100% 4.950 2.970 N PODN n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017000294.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09507 pDONR223 100% 95.2% 94.4% None (many diffs) n/a
2 ccsbBroad304_09507 pLX_304 0% 95.2% 94.4% V5 (many diffs) n/a
3 TRCN0000479035 CTTGATCTACGCTTGTCTGCTGAA pLX_317 19.7% 95.2% 94.4% V5 (many diffs) n/a
4 ccsbBroadEn_14372 pDONR223 100% 61.4% 61.1% None (many diffs) n/a
5 ccsbBroad304_14372 pLX_304 0% 61.4% 61.1% V5 (many diffs) n/a
6 TRCN0000468136 CCCAGTTGCAGAGTGTCGAATTCC pLX_317 34.7% 61.4% 61.1% V5 (many diffs) n/a
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