Construct: ORF TRCN0000468174
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007619.1_s317c1
- Derived from:
- ccsbBroadEn_01799
- DNA Barcode:
- GGCTGATACACAGACCGACTATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZKSCAN1 (7586)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468174
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_001346581.2 | 100% | 100% | |
| 2 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_003439.4 | 100% | 100% | |
| 3 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | XM_011516559.3 | 100% | 100% | |
| 4 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_001287054.3 | 93.6% | 93.6% | 0_1ins108 |
| 5 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_001346580.2 | 93.6% | 93.6% | 0_1ins108 |
| 6 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_001287055.2 | 62.1% | 62.1% | 0_1ins639 |
| 7 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NM_001346579.1 | 51.5% | 43.8% | (many diffs) |
| 8 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | NR_144477.2 | 17.4% | 1_219del;1019_1268del;2159_9653del | |
| 9 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | XR_927529.2 | 16.6% | 1_234del;1034_1758del;2649_10143del | |
| 10 | human | 7586 | ZKSCAN1 | zinc finger with KRAB and S... | XR_002956485.1 | 16.1% | (many diffs) | |
| 11 | mouse | 74570 | Zkscan1 | zinc finger with KRAB and S... | NM_133906.4 | 85.5% | 89.7% | (many diffs) |
| 12 | mouse | 74570 | Zkscan1 | zinc finger with KRAB and S... | NM_029869.1 | 74.3% | 78.5% | (many diffs) |
| 13 | mouse | 74570 | Zkscan1 | zinc finger with KRAB and S... | XM_006504628.3 | 74.3% | 78.5% | (many diffs) |
| 14 | mouse | 74570 | Zkscan1 | zinc finger with KRAB and S... | XM_011240958.2 | 53.1% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1758
- ORF length:
- 1689
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatgactgct gaatcacggg aagccacggg tctgtcccca caggctgcac 121 aggagaagga tggtatcgta atagtgaagg tggaagagga agatgaggaa gaccacatgt 181 gggggcagga ttccacccta caggacacgc ctcctccaga cccagagata ttccgccaac 241 gcttcaggcg cttctgttac cagaacactt ttgggccccg agaggctctc agtcggctga 301 aggaactttg tcatcagtgg ctgcggccag aaataaacac caaggaacag atcctggagc 361 ttctggtgct agagcagttt ctttccatcc tgcccaagga gctccaggtc tggctgcagg 421 aataccgccc cgatagtgga gaggaggccg tgacccttct agaagacttg gagcttgatt 481 tatcaggaca acaggtccca ggtcaagttc atggacctga gatgctcgca agggggatgg 541 tgcctctgga tccagttcag gagtcctcga gctttgacct tcatcacgag gccacccagt 601 cccacttcaa acattcgtct cggaaacccc gcctcttaca gtcacgagct cttcctgctg 661 cccacattcc tgcaccccct catgagggta gtcccagaga ccaggcgatg gcatctgcac 721 tattcacagc ggattcccag gcaatggtga agatcgagga catggctgtg tccctcattc 781 tggaggaatg gggatgtcag aatctggctc ggaggaatct cagtagggac aacaggcagg 841 agaattatgg gagcgcattt ccccagggtg gtgaaaacag gaatgagaac gaggagtcaa 901 cctcaaaggc tgaaacctcg gaagattcag catcacgcgg ggagacaaca ggaagatccc 961 agaaagagtt tggagagaaa cgtgaccagg agggcaaaac aggagaaaga cagcagaaaa 1021 accctgagga gaaaaccagg aaagagaaaa gagattcagg gccagctata ggaaaggaca 1081 aaaaaaccat cacaggagag agaggtccaa gggagaaggg gaaaggattg ggaagaagct 1141 tcagtctgag ctccaacttc accacccctg aagaagttcc cacgggaaca aagtctcaca 1201 gatgtgatga atgtggtaaa tgcttcacga gaagttcaag ccttatccgc cataaaataa 1261 tccacactgg agaaaagccc tatgaatgta gtgagtgtgg gaaagccttc agtcttaact 1321 ccaaccttgt cctgcatcag aggatccaca caggagagaa acctcatgaa tgtaacgagt 1381 gtggcaaggc cttcagccac agttccaatc tcaTCCTCCA TCAGCGCATC CACTCTGGAG 1441 AGAAACCTTA TGAATGTAAT GAGTGCGGGA AGGCCTTCAG CCAGAGCTCG GACCTCACCA 1501 AGCATCAGAG AATTCACACG GGGGAGAAAC CCTATGAATG TAGTGAATGT GGAAAAGCTT 1561 TCAACCGAAA CTCATACCTG ATTTTGCATC GGAGAATTCA CACTCGAGAA AAGCCCTACA 1621 AGTGCACTAA GTGTGGCAAG GCCTTCACCC GCAGCTCCAC CCTCACTCTG CATCACAGAA 1681 TCCATGCCAG AGAGAGAGCC TCTGAGTACA GCCCAGCCTC CCTTGATGCA TTTGGCGCGT 1741 TCCTGAAAAG TTGTGTGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1801 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1861 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGCTGAT ACACAGACCG 1921 ACTATGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt